LOCUS       Exported                3084 bp ds-DNA     circular SYN 07-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      218..245
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      337..423
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     487..503
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    543..638
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        640..658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..682
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     682..706
                     /label=XhoI_ICAM2_Full_FWD
     primer_bind     682..706
                     /label=oJDW 1776
     promoter        688..1084
                     /label=human ICAM2 full length promoter
     misc_feature    720..1053
                     /label=340 bp minimal pro
     misc_feature    729..738
                     /label=NFkB
     misc_feature    812..820
                     /label=SP1
     misc_feature    862..867
                     /label=GATA
     misc_feature    871..876
                     /label=ETS
     misc_feature    879..884
                     /label=ETS
     promoter        915..1053
                     /label=ICAM2 mini promoter
     misc_feature    936..941
                     /label=ETS
     misc_feature    954..959
                     /label=GATA
     misc_feature    964..973
                     /label=ETS
     iDNA            1009..1011
                     /label=TSS
     primer_bind     complement(1064..1083)
                     /label=REV
     primer_bind     complement(1068..1091)
                     /label=SacII_ICAM2_Full_FWD
     primer_bind     complement(1068..1091)
                     /label=oJDW 1777
     misc_feature    1089..1098
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(1111..1129)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    1129..1253
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1326..1344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1349..1365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1478..2287
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
       61 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
      181 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      241 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      301 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
      361 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
      421 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
      481 gacgttgtaa aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct
      541 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
      601 atgctttttt ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc
      661 gaattgggta ccgggccccc cctcgaggtc tcccaggcat gactccaaca atgcatccca
      721 tgggatttgg ggttccccag atctggggct tgtaggcctg actctcccct gtgcacacgt
      781 ctcatacacg catgcgtgca cccattgcct gccccgcccc ttgcacaggg agtcagcagg
      841 gaggactggg ttatgccctg cttatcagca gcttcccagc ttcctctgcc tggattctta
      901 gaggcctggg gtcctagaac gagctggtgc acgtggcttc ccaaagatct ctcagataat
      961 gagaggaaat gcagtcatca gtttgcagaa ggctagggat tctgggccat agctcagacc
     1021 tgcgcccacc atctccctcc aggcagccct tggctggtcc ctgcgagccc gtggagactg
     1081 ccagccgcgg tggagctcca gcttttgttc cctttagtga gggttaattc aagtttgtac
     1141 aaaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     1201 cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact atgaaccaac
     1261 tacttagatg gtattagtga cctgtagaat tcgagctcta gagctgcagg gcggccgcga
     1321 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1381 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1441 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1501 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1561 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     1621 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1681 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1741 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     1801 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1861 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     1921 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     1981 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     2041 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     2101 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     2161 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     2221 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     2281 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     2341 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgtt
//
