LOCUS       Exported                3471 bp ds-DNA     circular SYN 30-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     668..728
                     /label=oJDW XXXX (p5E FWD)
     primer_bind     668..728
                     /label=oJDW 2185
     misc_feature    698..889
                     /locus_tag="SV40 polyA"
                     /label=SV40 polyA
     polyA_signal    707..828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    772..791
                     /label=SV40pA REVSeq
     misc_feature    902..993
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     942..961
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     misc_feature    1007..1253
                     /label=NOS terminator
                     /note="nopaline synthase terminator from Agrobacterium
                     tumefaciens (NOS terminator)"
     misc_feature    1273..1392
                     /label=C120 regulatory element
     misc_feature    1273..1292
                     /label=20bp version of Clone-1
     misc_feature    1298..1317
                     /label=20bp version of Clone-1
                     /label=20bp version of Clone-1(1)
     misc_feature    1323..1342
                     /label=20bp version of Clone-1
                     /label=20bp version of Clone-1(2)
     misc_feature    1348..1367
                     /label=20bp version of Clone-1
                     /label=20bp version of Clone-1(3)
     misc_feature    1373..1392
                     /label=20bp version of Clone-1
                     /label=20bp version of Clone-1(4)
     promoter        1400..1492
                     /label=mmu-cFos min pro
                     /label=cFos
                     /note="murine c-Fos minimal promoter"
     primer_bind     1434..1456
                     /label=oJDW 1408 (c-fos min pro FWD)
     primer_bind     complement(1474..1492)
                     /label=oJDW 1983 (c-fos min pro REV)
     primer_bind     complement(1487..1534)
                     /label=oJDW XXX (p5E REV)
     primer_bind     complement(1487..1534)
                     /label=oJDW 2186
     protein_bind    1517..1640
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1736..1752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1865..2674
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2821..3409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgga attctagcag acatgataag atacattgat
      721 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      781 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      841 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta tcgatagtac
      901 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
      961 ccagtgcaag tgcaggtgcc agaacatttc tctcctcgag agatctttaa gattgaatcc
     1021 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     1081 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     1141 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     1201 gcgcgcggtg tcatctatgt tactagatcg ggaattgatc ccccctcgac agcggtacca
     1261 cgcgttgagc tctaggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt
     1321 tataggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt tataggtagc
     1381 ctttagtcca tgcgtcgacc cagtgacgta ggaagtccat ccattcacag cgcttctata
     1441 aaggcgccag ctgaggcgcc tactactcca accgcgactg cagcgagcaa ctaacgcggc
     1501 cgcactagtt ctagatcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata
     1561 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     1621 caacatatgc agtcactatg aatcaactac ttagatggta ttagtgacct gtagaattcg
     1681 agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat
     1741 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1801 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1861 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     1921 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1981 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2041 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2101 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2161 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2221 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2281 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2341 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2401 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2461 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2521 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2581 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2641 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     2701 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     2761 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2941 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3181 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     3241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
