LOCUS       pea038___Nipbl_B        5378 bp ds-DNA     circular     08-NOV-2021
DEFINITION  .
ACCESSION   L09137 X02514                                                      
VERSION     L09137.2  GI:20141090                                              
SOURCE      Cloning vector pUC19c                                              
  ORGANISM  Cloning vector pUC19c other sequences; artificial sequences;
            vectors.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(5220..5248)
                     /locus_tag="Amp Promoter (from Serial Cloner)"
                     /label="Amp Promoter (from Serial Cloner)"
                     /ApEinfo_label="Amp Promoter (from Serial Cloner)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1898..2218
                     /locus_tag="FKBP-V"
                     /label="FKBP-V"
                     /ApEinfo_label="FKBP-V"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1351..1363)
                     /locus_tag="sgRNA"
                     /label="sgRNA"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1760..1765
                     /locus_tag="NheI site"
                     /label="NheI site"
                     /ApEinfo_label="NheI site"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3139..3144
                     /locus_tag="NsiI"
                     /label="NsiI"
                     /ApEinfo_label="NsiI"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1766..1828
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3541..4223)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1892..1897
                     /locus_tag="AG linker"
                     /label="AG linker"
                     /ApEinfo_label="AG linker"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2267..2270)
                     /locus_tag="sgRNA(1)"
                     /label="sgRNA(1)"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1865..1891
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1367..1367
                     /locus_tag="eGFP"
                     /label="eGFP"
                     /ApEinfo_label="eGFP"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3153..3173)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2219..2263
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4321..4980)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(3179..3201)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    402..1363
                     /locus_tag="Nipbl telomeric homology arm"
                     /label="Nipbl telomeric homology arm"
                     /ApEinfo_label="Nipbl telomeric homology arm"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2258..2263
                     /locus_tag="BspEI"
                     /label="BspEI"
                     /ApEinfo_label="BspEI"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(239..307)
                     /locus_tag="LAcZalpha (serialcloner)"
                     /label="LAcZalpha (serialcloner)"
                     /ApEinfo_label="LAcZalpha (serialcloner)"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2264..3138
                     /locus_tag="Nipbl homology arm"
                     /label="Nipbl homology arm"
                     /ApEinfo_label="Nipbl homology arm"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    994..1018
                     /locus_tag="EA077"
                     /label="EA077"
                     /ApEinfo_label="EA077"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1835..1861
                     /locus_tag="HA tag"
                     /label="HA tag"
                     /ApEinfo_label="HA tag"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1368..1759
                     /locus_tag="Blast"
                     /label="Blast"
                     /ApEinfo_label="Blast"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtTCGCG AGAAACAGGG GAAGATGGAC
      421 AGGCGAGATA GCTCAGCAAG GAAAAGCACC AACCACAAGC CTGAGCTGCT GAGTACAGTC
      481 CTCCAGACTC TTGTGCTGAG GGAAAGATCC TAGTTCCGCA GATTATCTTC TGATCTGCAC
      541 ACACATCAGT CATAGCAGGC ACACAACAAT ACACGTGGAC ACTAAGTGAA CAAATAGTAA
      601 CAAATTACAA ATGGGGCAGG GGAGGAGGAG GGACATATCA AGTGTGTCAC AAAGGAATAT
      661 ACAGCAATTT ACTGAGAAAA TATTGGGCAA TAAATGTCAT AAATTCAGTT ATCCTAAGGT
      721 AAAATGGCTG TGGAATGAAA GTGTTCTTGA ATTGTAGGAT TTAGAAGAGA GGAGAATAAA
      781 AGTCTCACAC TGCAAACCAC AAGGATCCAA AAGAATCTGA AGAGATACTC TTGGTAGGAG
      841 GGGCAAGTCC ATTTAGAGAG TAAAAAGGGA ACTAGACAAG AAATTGAAGC CAGATTATTA
      901 AGTAAAAATT TTATTGTATT TAGAGTTTAG AAAGAGTACC CTGCCCTAGC AGTATATACA
      961 CCAGAAGTTA AGAAACAAGA TTCTGGAGCT AGATTGTGTC ATTAGAATTA CAGCTCCAGT
     1021 AGTTACTTTA AACTCACTGT AATTTTCTCA TTAGTAAAAT GCAGAAAAGA GTAATACTCT
     1081 GCTGTACCTG GGTTGTTGTG AGACCTAAAT CTTAATGTAT ACAAAGCATT TTGCAGTGTT
     1141 TAATGTATGG CACATGCTTG TTAAACACAT TGAACAAATA GAAGCTAAGA GTTTTGTTTC
     1201 TAAATGAATA AGTATCTCTC TGCTACTCAT ATATTTGACA AAGTTCTACA AATAATTATA
     1261 TGGTTTTCTT TGTGTGTGTT GCAGAGTTTG GGAAATGGAA AGTAATGACA GCTGGCACCT
     1321 GAACTAAATA CTTTCATAGA CAACACCATT CCAGAACTTC AGGATGgcca agcctttgtc
     1381 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     1441 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     1501 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     1561 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     1621 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     1681 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     1741 tggttatgtg tgggagggcg ctagcggcag tggagagggc agaggaagtc tgctaacatg
     1801 cggtgacgtc gaggagaatc ctggcccagg taccTACCCA TACGACGTAC CCGATTATGC
     1861 Tggctacccc tacgacgtgc ccgactacgc cGCCGGCgga gtgcaggtgg aaaccatctc
     1921 cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg
     1981 gatgcttgaa gatggaaaga aagttgattc ctcccgggac agaaacaagc cctttaagtt
     2041 tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt
     2101 gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc
     2161 aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaag
     2221 tggtggcggt ggctcgggcg gtggtgggtc gggtggctcc ggaATGAATG GGGATATGCC
     2281 CCATGTCCCC ATAACTACGC TTGCGGGGAT TGCTAGTCTT ACGGACCGTA AGTTTGCATA
     2341 ATTTTCCTAA GTTCTTTACT TTGCTAATAC TCATTTTTAG AGACAAAAAA TTACCTTTAA
     2401 TGTCACATAT AACAGAAAAT ATGTATTTGA TTAGTGTGGG GGGAGTAGCA AAATTAATGT
     2461 TTCCACCTAA TACATAAATT TAATTTCTTC ATCCTTCTGT GAACTCCTAA AGAAATGATA
     2521 GTGTGATAAT TTTTATAAAG ACCTTAAAAA TCAATAAGGA CTTTAGAAAA ATGACACAAA
     2581 GCTTTAAGAG ATGTTAAAAC TTAAATAAAC ATGGGTTAAT AAACAAAACT ATGGTCTTTT
     2641 TTTTAAGAAA GCGTTTACAA AAACAGTACC TGAAAGTGTC CTCCAAGAAT GAGAAATTGA
     2701 TCGCCCACAT TACCTAAACT GTAGTGGTCC TGATTTTTTT AATATCAGGT TGAATTGTTA
     2761 GTTAGAATTT GTTAACAGGT ATGAGATATG TCATCACTAT GAGAGCCCAC TTTACTCTGA
     2821 ACACTAAGGT ATTAAAAATT ATTGTCAATA CTTAGAAATT TAGTTAATAG AAGTTTTAAG
     2881 GTTCATATTT TGGTAGACAC ATAACTATTC TTTTCCTTGG GTAATGGCAC AGAGTCTGCC
     2941 CAAGTGTTTG TCCCTATGTC ATCCTCCTTT TAGTATAGGA ATAGAGATAA AGGAAAGGCC
     3001 AGAAAAGAAA AAACAAAATG AAAACATAAA TACCTTTTTC TTATAGCAGT ATGTAAGATA
     3061 TGGAACTTTG CAATAATCTA TGAAAAAATA ACTTCCAGAA CTGTTTTCAA CTGTGCTCAG
     3121 AATCCCTCCC TGGGTTTTAT GCATggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5281 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5341 aacctataaa aataggcgta tcacgaggcc ctttcgtc  
//
