LOCUS       VPS13B^HaloCodon       18991 bp DNA     circular SYN 23-JUL-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 18991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2024 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18991
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    735..4661
                     /label=VPS13B
                     /note="This forward directional feature has 2 segments:
                        1:  735 .. 4653 / #a6acb3
                        2: 4654 .. 4661 / #a6acb3"
     misc_feature    2553..2585
                     /label=second FFAT change
                     /note="color: #a6acb3"
     misc_feature    3237..3272
                     /label=possible FFAT change
                     /note="color: #a6acb3"
     CDS             4662..5552
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /note="color: #cc99b2"
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    5553..5559
                     /label=Linker_BAMH
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5560..13736
                     /label=VPS13B
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    13767..14355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(14238..14249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 14237"
                     /translation="IEGR"
     polyA_signal    14421..14469
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      14671..15099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        15113..15442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      15293..15428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             15509..16303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    16479..16600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(16649..16665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    16673..16689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(16697..16727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 16697 .. 16703 / #ffffff / -10
                        2: 16704 .. 16721 / #ffffff
                        3: 16722 .. 16727 / #ffffff / -35"
     protein_bind    16742..16763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(17051..17639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(17810..18670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 17810 .. 18601 / #ccffcc
                        2: 18602 .. 18670 / #ccffcc / signal sequence
                     Cleavage site after base 18601"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(18671..18775)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc caccatgctc gagtcttacg ttacacccat cctcatgagt tacgtaaata
      781 gatacatcaa aaatctgaag ccttctgact tacagctgag tttgtggggc ggcgacgtgg
      841 tcctgtcgaa gctagaattg aagcttgacg tgctagaaca ggaactgaaa ctccccttca
      901 ccttcttgtc gggccacatc cacgagctca ggatacatgt gccatggacc aagctgggta
      961 gcgagcctgt ggtcattaca atcaacacta tggaatgtat cctcaagctg aaggatggca
     1021 tccaagacga ccacgagagc tgcggcagta actctaccaa cagaagcaca gccgagtcga
     1081 ccaagagctc tatcaagcca cggcggatgc agcaggccgc ccccactgac cccgacctcc
     1141 cgcctggtta tgtacagtcc ctgatcagac gagtggtgaa caacgtgaac atcgtgatca
     1201 acaacctaat cctgaagtac gtggaagacg acattgttct gtctgtgaac atcacatctg
     1261 ccgaatgcta caccgtgggg gaactgtggg accgggcttt catggacata agtgccacag
     1321 acctggtgct ccgcaaggtg ataaatttca gcgactgcac cgtgtgtctt gataagagaa
     1381 atgcctctgg caaaatcgag ttctaccaag atcccctcct gtacaagtgc tctttcagaa
     1441 ctagactaca cttcacctac gagaatttga acagcaaaat gccctcagtc attaagatcc
     1501 acacactggt ggaatcgcta aagctgagta tcaccgacca acaattacct atgtttatca
     1561 ggatcatgca gctgggaatc gccctgtact acggtgagat cggcaacttc aaggagggcg
     1621 agatcgagga cctgacatgt cacaacaagg atatgctggg caacatcacc ggatctgagg
     1681 acgagactcg gatcgacatg cagtaccccg cccagcacaa gggccaggaa ttgtactctc
     1741 aacaggatga agagcaaccg cagggatggg tttcctgggc ttggtcattc gtgcccgcca
     1801 tcgtctctta tgatgacgga gaggaagact tcgtcggaaa tgaccctgcc tcaaccatgc
     1861 accagcagaa agcccagacc ctgaaggacc ctattgtgtc cataggcttt tactgtacca
     1921 aagccaccgt caccttcaag ttaacggaga tgcaagtgga atctagttac tatagccctc
     1981 agaaggtgaa aagcaaggaa gtattatgtt gggaacagga aggcaccacc gtagaggccc
     2041 tcatgatggg cgagcccttt ttcgattgtc aaatcggctt cgtgggatgc agggccatgt
     2101 gcctaaaggg catcatgggc gtgaaagact ttgaagagaa catgaataga tcagaaacag
     2161 aagcctgctt cttcatttgc ggcgataatt tgtccacaaa aggctttaca tacctgacca
     2221 actccctctt tgactataga agccctgaga acaacggcac cagagctgag ttcatactcg
     2281 atagtaccca tcacaaagag acatacaccg aaatcgccgg catgcagcgg ttcggcgcct
     2341 tttacatgga ctacctgtac accatggaaa acaccagcgg aaaaggtagc acaaaccagc
     2401 aggactttag ctccggaaag tccgaagacc tcggtacagt ccaggagaag agcacaaaga
     2461 gcctagtaat cggccctctg gacttccggc tcgactcctc tgccgtgcac agaatcctga
     2521 aaatgatcgt ctgtgccctg gagcacgagt acgaacctta ctctcggctt aaaagcgaca
     2581 ttaaggacga gaacgagacc atcctgaacc ctgaggaggt ggcgctgtta gaagagtaca
     2641 tccctaccag acacacttct gtgacactac tgaagtgcac ctgtacaatc agtatggctg
     2701 aattcaatct gctggaccac ctgctgcccg tcatcatggg cgagaagaac tcctccaatt
     2761 tcatgaatac taccaatttc cagagcctga gaccccttcc tagcatcaga attctagttg
     2821 ataagatcaa cctagagcac tctgtgccaa tgtacgccga gcagcttgtc cacgtggtat
     2881 cttcactcac ccagccttca gataacctgc tgcattactg ttacgttcac tgctacctga
     2941 agatcttcgg cttccaagca ggcctgacat cgctcgactg ctctggatca tactgcctcc
     3001 cagtccccgt gatccccagt ttttcgaccg ctctgtacgg aaagttactg aagctgccaa
     3061 cctgttggac caagcgtagt cagatcgcca tcaccgaagg aatcttcgag ctgcctaacc
     3121 tgacaatcca ggccaccaga gctcagacgc tgttgctcca agccatctac cagtcttggt
     3181 cccacctggg caacgtgagc tcctctgccg tgatcgaggc cctgatcaac gagatctttt
     3241 tgagtatcgg cgtgaaaagc aagaaccctc tgcctacact ggaagggagc atccagaacg
     3301 tggagctgaa atactgttca acaagccttg tgaaatgcgc cagcgggacc atgggaagca
     3361 tcaagatttg cgctaaagct ccggtggaca gcggcaagga aaaactgata ccactgctgc
     3421 agggcccgag cgacaccaaa gacctccaca gtactaagtg gctgaatgag agccgaaaac
     3481 ctgagagtct ccttgccccc gatctgatgg ccttcaccat ccaggtgccc caatacatcg
     3541 actactgcca caattcaggg gctgtgttac tgtgcagcat tcagggactg gctgtgaaca
     3601 tcgaccccat tctgtacacc tggctcatct atcagccaca aaagagaacc agtcgtcaca
     3661 tgcagcagca gcccgtggta gccgtgcctc tcgtgatgcc agtgtgtcgc cggaaagagg
     3721 acgaggtgtc tatcggcagc gctcctctcg caaagcagca gtcttaccag gcctcggagt
     3781 acgcctctag ccctgtgaag accaagaccg tcactgaatc tcgacccctt tcggtccccg
     3841 tgaaagccat gcttaacatc tctgagtcct gcagaagtcc ggaggagcgg atgaaggaat
     3901 tcatcggtat tgtgtggaat gccgtgaaac atctcacact gcaactcgag gtgcaaagct
     3961 gctgtgtgtt catccccaac gactctttgc ctagcccttc caccatcgtg tctggcgata
     4021 tcccggggac tgtccggagc tggtaccacg gccagaccag tatgcccggc actctagtac
     4081 tctgtcttcc acagattaaa attatctctg ccggacacaa gtacatggaa cctctccagg
     4141 aaatcccatt cgtgatccct agacctatcc tggaagaagg cgatgcgttc ccttggacaa
     4201 tctccctcca taatttttcg atctatacac tgctgggaaa gcaggttacg ctgtgccttg
     4261 tggagccaat gggttgcaca tctaccctgg ccgtgacctc gcagaaactg cttgccaccg
     4321 gaccagacac ccggcactca ttcgtagtct gcttgcatgt ggacttagag tccctcgaaa
     4381 tcaaatgctc taacccgcag gtgcagctgt tttacgaact gaccgatatt atgaacaagg
     4441 tgtggaacaa aatacagaaa cggggcaacc ttaacctgag cccaactagc ccagagacaa
     4501 tggctgggcc tgtgcctacg agtcccgtga gatccagcat cggtaccgcc cctcctgaca
     4561 cttctacctg ttcgccttcc gctgacatcg gcacaactac cgagggggac agtatacagg
     4621 ctggcgagga gtctcctggc tctggctctt caggatccgg catggcagaa atcggtactg
     4681 gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg
     4741 ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg
     4801 tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga
     4861 tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct
     4921 tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact
     4981 ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg
     5041 catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg
     5101 agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg
     5161 tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg
     5221 accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa
     5281 acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg
     5341 actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga
     5401 tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca
     5461 tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg
     5521 cgcgctggct gtcgacgctc gagatttccg gcggcggcgg atccggcgga ttcagtgata
     5581 gcgtgaccct tgaacagacc acttccaata tcggtggtac cagtggccgg gtgtctctgt
     5641 ggatgcagtg ggtcttgcct aaaatcacaa ttaagctgtt cgcccctgac cccgagaata
     5701 agggtaccga agtgtgcatg gtgtctgaac tggaggacct gtccgccagc attgacgtgc
     5761 aggacgtgta caccaaggtc aagtgcaaga tcgagtcctt taacatagac cactaccgct
     5821 cttctctggg ggaggaatgc tggtccctgg gccagtgtgg cggcgtgttt cttagctgca
     5881 ccgataagct caacagacgc accctccttg tgagacctat ctccaagcaa gaccccttca
     5941 gtaactgctc cggatttttt cctagcacca ccacaaagct gctggacgga acccatcagc
     6001 aacacggctt cttatctctg acctacacca aggctgtcac caagaacgtg agacataagc
     6061 tgaccagccg gaatgagaga agatctttcc acaagctatc cgaagggctc atggacgggt
     6121 ccccccactt cctgcacgaa atactgctct ccgcccaagc cttcgacata gttctgtact
     6181 ttcccctact gaacgccatc gccagcatct tccaggccaa gctgcctaag acacaaaagg
     6241 aaaagcggaa gagccccgga cagccaatgc ggactcacac actcaccagc agaaacctgc
     6301 ccctcatcta cgtgaatact tcggtgatca gaatctttat ccccaagaca gaagagatgc
     6361 agccgaccgt ggaagccaac caggccgcca aagaagatac agtggttcta aaaatcggct
     6421 ctgttgcaat ggccccgcag gccgacaacc ctctgggaag aagcgtgctg agaaaggata
     6481 tctatcagcg ggccctgaac ctgggcatac tgagagaccc tggaagcgaa atcgaagacc
     6541 gacagtacca gatcgatctg cagagcatca acatcggcac ggcccaatgg caccagctga
     6601 agcctgagaa ggagtctgtg tccgggggcg tcgttacaga gaccgaaaga aactcccaaa
     6661 accccgccct ggagtggaac atggcgagca gcataaggcg gcaccaggag agaagagcca
     6721 tcctgacccc tgtgctcaca gatttctcag taagaatcac cggcgctccc gcagtgattt
     6781 ttactaaagt ggtatctcct gaaaacctcc atacagaaga aatcctcgtc tgcggccaca
     6841 gtctcgaggt gaatatcact accaacctcg acttcttcct gtctgtcgca caggtccagc
     6901 tgctgcacca gctgattgtg gccaatatga caggcctgga acccagcaac aaggctgccg
     6961 aaatttccaa gcaggaacag aagaaggtcg atatatttga cggaggcatg gccgagacaa
     7021 gcagccggta cagtggcgca caggacagcg gcattggatc tgatagtgtc aagatcagaa
     7081 tagtgcagat cgagcagcac agcggcgcct ctcagcatag gattgcaaga ccgtccagac
     7141 agtcaagcat cgtgaaaaac ctgaacttca tcccctttga cattttcatc accgcctcaa
     7201 gaatcagcct gatgacctat agctgcatgg ctttgagcaa gtcaaagtcc caagaacaga
     7261 agaacaacga aaagacagac aaatctagcc tgaacctacc cgaagtagat agcgatgtgg
     7321 ccaagcctaa tcaggcctgc atcagcacag tcacagctga ggatctactg agatcctcca
     7381 tctcttttcc tagtggtaaa aaaatcgggg tcttatcatt ggaatcgctg cacgcctcca
     7441 ctagaagctc tgcacggcag gctctaggca tcacaatcgt gagacagcct ggcagaagag
     7501 gtacaggcga cctgcagctc gagcccttcc tttatttcat cgtgtcccag cctagtctcc
     7561 tgctgtcgtg ccatcacaga aagcagagag tggaggtttc catcttcgac gccgttctaa
     7621 agggcgtcgc ctctgactac aagtgtattg acccgggaaa aaccctgccc gaggcacttg
     7681 attactgtac cgtgtggctg caaaccgtgc ccggcgagat tgactccaag tccggaatcc
     7741 ctcccagttt cattacactg caaatcaaag atttcctcaa cggccctgcc gacgtgaacc
     7801 tggacatcag taagcctctc aaagcaaacc tgagtttcac aaagctggat cagatcaacc
     7861 tgtttctgaa gaaaataaag aatgcccact cactcgctca ctctgaggaa acctctgcca
     7921 tgtctaatac catggtgaac aaagacgatc tgcccgtgtc aaagtactat agaggcaaac
     7981 ttagtaaacc taaaattcac ggcgatggag tacaaaagat ctcggcccag gaaaatatgt
     8041 ggcgagctgt ctcctgcttc cagaaaattt ccgttcagac gacccagatc gtcatcagta
     8101 tggaaaccgt tcctcacacc tcgaagcctt gtctgctcgc ttctctgtcc aatctgaacg
     8161 gctccctgtc agtgaaggcc acacagaaag tgcccggaat cattctgggc tcgtctttcc
     8221 tgctgtcaat caacgacttc ctgctgaaga cctcccttaa ggaacggagc agaatcctga
     8281 ttgggccatg ctgcgccaca gcgaacctgg aagctaaatg gtgcaagcac tctggaaatc
     8341 ctggtcctga gcaatctatc ccaaagatca gtatcgacct taggggcggc ctgctgcaag
     8401 tgttctgggg acaggagcac ctcaattgcc tggtgctgct gcacgagtta ctgaatgggt
     8461 acctaaatga ggagggcaac ttcgaggtac aggtgagtga gcctgtgcca cagatgagca
     8521 gcccggttga gaagaatcag acattcaaga gcgagcagag ctcagatgat ctcagaactg
     8581 gcctgttcca gtacgttcag gatgccgaga gcctgaagct gcctggagtg tacgaagttc
     8641 tattctacaa cgagactgaa gactgccccg gcatgatgct ctggcggtac ccagaaccta
     8701 gagtgctgac cctggtgcgc atcacaccag tgccattcaa caccaccgaa gatcctgata
     8761 tcagtaccgc cgacctgggg gatgtgctgc aggtcccttg cagcctcgaa tactgggatg
     8821 agctgcagaa ggtattcgtg gcctttagag agttcaatct gtctgaatca aaggtgtgcg
     8881 agctgcaatt acctgacatc aacctcgtta acgaccagaa gaaactggtt tctagcgacc
     8941 tgtggcggat cgttcttaac agctcacaaa acggcgccga tgatcagtcc agtgcctcgg
     9001 aatctggctc ccagtctacc tgcgaccctc tcgtcactcc gaccgcgctc gccgcctgca
     9061 cacgggtgga tagctgcttt accccatggt tcgtgccctc tctgtgcgtg agcttccagt
     9121 tcgcccactt ggaatttcac ttatgccacc atctggatca actgggcacc gccgcccccc
     9181 aataccttca gcccttcgtg agcgaccgga acatgcctag cgagttggag tacatgattg
     9241 tgtctttcag agagccccat atgtacctga gacagtggaa caacgggtct gtatgccagg
     9301 agatccaatt cctggcccag gcggactgca agctcctgga gtgcagaaac gtgacaatgc
     9361 agagtgtggt gaaacctttt tctatcttcg gtcagatggc cgtgtcctcc gacgtggtgg
     9421 agaaactgtt agattgtacc gttatcgtag acagcgtctt tgtaaacctg ggccagcatg
     9481 tggttcattc gttgaacact gcgatccagg cttggcagca aaataagtgc cctgaagtgg
     9541 aggaactggt gttctcgcat ttcgtgatct gtaacgacac acaggagacc ctgagattcg
     9601 gccaggtaga caccgacgaa aatattttgc ttgcctctct acacagtcac caatactcct
     9661 ggagatccca caagtcccct caactcctgc acatctgcat cgagggatgg ggcaactggc
     9721 ggtggagcga gccattctcc gtggaccacg ccggcacttt tattcggacc atacaatatc
     9781 gggggagaac cgctagcctc atcatcaagg ttcagcagct gaacggggtc cagaagcaaa
     9841 tcattatctg tggccgtcaa atcatctgct catacctgtc tcagtccatc gagctgaagg
     9901 tggtgcagca ctacattggc caagatggcc aggccgtagt tcgggaacac ttcgattgcc
     9961 tgaccgccaa gcagaagctg ccttcctaca tcctggagaa caatgagctt acagagctgt
    10021 gcgtgaaggc gaagggggac gaggactggt ccagagatgt ttgtttagaa agcaaagcac
    10081 cggaatactc tatcgtaatc caggtgccat ctagcaactc gtctatcatc tacgtgtggt
    10141 gcaccgtgct gacactggag cccaacagcc aagtgcagca aagaatgatc gtgttttccc
    10201 cactgttcat catgagatcc cacctgccag acccaatcat catccaccta gaaaagagat
    10261 ctctcggcct gtccgagact cagatcatac ctggtaaggg ccaagaaaaa ccactgcaga
    10321 atatcgagcc tgatctggtg caccacctga ccttccaggc tagagaagag tacgatccct
    10381 ccgactgcgc cgtcccaatc tctacctctc ttatcaagca gatcgcaaca aaagtgcacc
    10441 ccggcggcac cgtcaaccag atcctggacg agttctacgg ccctgaaaag agcctgcagc
    10501 ccatctggcc ttacaacaaa aaggattccg acagaaacga gcagctgtca cagtgggaca
    10561 gtccaatgcg ggtgaagtta tcaatctgga aaccctacgt gcggacgtta ctgatagagc
    10621 tgttaccttg ggcgttgctg ataaacgagt caaagtggga cctgtggctg tttgagggcg
    10681 agaaaatcgt gcttcaagta ccagccggca agatcattat ccctccaaac ttccaggagg
    10741 cctttcagat cggcatctac tgggccaaca ccaacaccgt ccataagtct gtggccatta
    10801 agttagtgca taacttgacc tctcctaaat ggaaggacgg cggcaacgga gaggttgtga
    10861 ccctggacga agaggccttc gtggacaccg agatcagact tggtgcattc cctggccacc
    10921 agaagctgtg tcagttctgc atcagctcca tggtccagca gggcatccag atcattcaga
    10981 ttgaagacaa gacaaccatt atcaacaaca caccctacca gatcttctac aaacctcagt
    11041 tatccgtgtg taaccctcac agcggcaagg aatatttccg agtgcctgat tctgccacgt
    11101 tctcaatctg ccctggcgga gagcagcctg ctatgaagtc ctcgtccctg ccttgctggg
    11161 atctgatgcc agacatctcc cagagcgtac tcgacgccag tctcctacag aaacagatca
    11221 tgctgggttt cagtccagct ccaggcgccg acagcagcca atgttggagc cttcctgcta
    11281 tagttcggcc cgaattccct cggcagagcg tggccgtgcc tttaggaaat ttcagagaaa
    11341 acggcttttg cacaagagcc attgtgctaa cataccaaga gcacctggga gtgacttacc
    11401 tgacgctgag cgaagatcca tctcccagag tcatcatcca caacagatgc cccgttaaga
    11461 tgctgattaa ggagaacatt aaggacatcc cgaagttcga agtgtactgc aagaaaatcc
    11521 cgtccgagtg cagcatacac catgaactct accaccagat ttccagctac cccgattgca
    11581 agacgaagga cctgctgccc agcttgctgc tgagagtgga acctctggac gaggtgacta
    11641 cagagtggtc tgacgccatc gacatcaaca gccagggcac acaagtagtt ttcttgacag
    11701 gatttggcta cgtctacgtc gatgtcgttc accagtgtgg cactgtgttc attactgtag
    11761 cccctgaagg gaaggctggc cccattctca ccaacaccaa cagagccccg gaaaaaattg
    11821 tgacctttaa gatgttcatc acacaactgt cattggctgt gtttgatgat ctgacccacc
    11881 acaaagccag cgcggagctc ctgcgactca ccttagataa catctttctg tgcgtcgccc
    11941 caggcgctgg cccactgcct ggcgaggaac ccgtcgccgc cctgttcgag ctgtattgcg
    12001 ttgagatttg ctgcggcgac ctacaactgg acaaccagct gtacaacaaa agcaatttcc
    12061 actttgccgt cctggtttgc cagggcgaaa aggctgaacc aatccagtgt agcaagatgc
    12121 agagcctgct tatcagcaac aaggaattgg aggagtacaa ggaaaagtgt ttcatcaagc
    12181 tgtgcattac cctgaacgaa ggcaaatcca tcctctgcga tatcaatgaa tttagcttcg
    12241 aactcaaacc agccagactg tacgttgagg atacattcgt gtactacatc aagactctct
    12301 tcgacacata cctgcctaac agccgtctag ccggacacag tacccacctg agcggtggca
    12361 agcaggtgct gcctatgcag gtgacccagc atgcccgggc cctggtcaac cccgtaaagc
    12421 tgagaaaact ggttatccag ccagtaaacc tgctggtgag catccatgcg tcgctgaagt
    12481 tatatatcgc cagcgaccat accccgctga gcttctctgt gtttgagcga ggacccatct
    12541 ttaccacagc cagacagctg gtgcacgccc tggcaatgca ctacgccgcc ggcgccctct
    12601 ttcgggctgg ctgggtggta ggcagcttgg atatcctcgg tagccctgcc agcctggtgc
    12661 gatccatcgg aaacggcgtg gctgacttct tccgactgcc ctacgaaggc ctgaccagag
    12721 gccctggcgc cttcgttagt ggcgttagca gaggcactac ctcattcgtg aagcacatct
    12781 ctaagggaac ccttacttca atcaccaact tagccacatc tctcgcaaga aacatggaca
    12841 gacttagcct cgatgaggaa cactacaacc ggcaggagga gtggcggaga cagctgcctg
    12901 agtcgctggg ggagggactg aggcagggcc tgtctcggtt aggcatcagc ctgttaggag
    12961 ccatcgcggg aatcgtggac cagcctatgc agaattttca gaagacttct gaagcccagg
    13021 ccagtgccgg ccacaaggct aagggagtca tcagcggcgt tggcaagggg atcatgggcg
    13081 ttttcactaa accaatcggg ggcgcagccg agctggtgag tcagacaggc tacggcattc
    13141 tgcacggcgc aggcctgtct cagctgccta aacaacggca tcagccatct gacctgcacg
    13201 ccgaccaggc tcccaacagc cacgtgaagt acgtgtggaa gatgctgcag agcctgggca
    13261 gacctgaggt gcatatggcc ctggacgtgg tgctcgtgcg gggcagcggt caggaacacg
    13321 agggctgcct gctgttaacc tccgaggtgc tgttcgtcgt gagcgtatcg gaggataccc
    13381 agcagcaagc ttttcctgtg accgagatcg actgcgccca ggattctaag cagaacaact
    13441 tactgacagt gcaactgaag cagcctagag tggcctgtga cgtagaagtc gacggtgtga
    13501 gagagagatt gtccgaacag cagtacaacc ggctggtgga ctacattacc aagacgagct
    13561 gccacctggc ccctagctgt agcagtatgc agatcccctg tcctgttgtg gccgccgaac
    13621 cccccccctc cacggtgaag acttaccact atctggtcga cccacatttc gcccaggtgt
    13681 tcctttccaa gttcaccatg gtgaaaaaca aggccctcag aaagggcttt ccctgaaccg
    13741 gttagtaatg agtttgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
    13801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
    13861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
    13921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
    13981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
    14041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
    14101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
    14161 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
    14221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
    14281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
    14341 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc
    14401 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
    14461 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
    14521 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
    14581 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc
    14641 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    14701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    14761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    14821 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    14881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    14941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    15001 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
    15061 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    15121 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    15181 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    15241 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    15301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    15361 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    15421 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    15481 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
    15541 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
    15601 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    15661 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
    15721 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    15781 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    15841 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    15901 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    15961 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    16021 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
    16081 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    16141 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    16201 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    16261 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
    16321 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    16381 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    16441 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    16501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    16561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    16621 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    16681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    16741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    16801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    16861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    16921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    16981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    17041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    17101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    17161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    17221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    17281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    17341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    17401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    17461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    17521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    17581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    17641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    17701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    17761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    17821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    17881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    17941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    18001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    18061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    18121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    18181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    18241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    18301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    18361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    18421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    18481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    18541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    18601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    18661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    18721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    18781 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc
    18841 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    18901 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    18961 gaccgacaat tgcatgaaga atctgcttag g
//
