LOCUS       PFBOH_BMI1              5708 bp    DNA     circular     07-Aug-2024
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     mobile_element     894..1118
                     /label="Tn7R"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     mobile_element     3744..3909
                     /label="Tn7L"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     polyA_signal     3581..3715
                     /label="SV40 poly(A) signal"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#cc99ff"
     promoter        4573..4677
                     /label="AmpR promoter"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        2268..2359
                     /label="polyhedrin promoter"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             4678..5538
                     /label="AmpR"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffc800"
     rep_origin      1..589
                     /label="ori"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     CDS             1185..1718
                     /label="GmR"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             2469..3443
                     /label="BMI1"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffc800"
     rep_origin      4093..4547
                     /label="f1 ori"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     promoter        1888..1916
                     /label="Pc promoter"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     775..826
                     /label="bom (fragment)"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             1894..2241
                     /label="YPR2_ECOLX (fragment)"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             3475..3558
                     /label="large T antigen (fragment)"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             4072..4088
                     /label="lacZ? (fragment)"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             2415..2435
                     /label="Feature 28"
                     /note="6x his tag"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             1801..2280
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             4678..5538
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             1028..1330
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             1910..2152
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             1323..1655
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             2415..3446
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     complement(5142..5408)
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     complement(3081..3314)
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     complement(1185..1871)
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     complement(2765..3046)
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     complement(1891..2199)
                     /label="ORF"
                     /note="ORF"
                     /ApEinfo_fwdcolor="#c8c8ff"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa 
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc 
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac 
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac 
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa 
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg 
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt 
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact 
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc 
     1321 tcatgcttga gcagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag 
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa 
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta 
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg 
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg 
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga 
     1681 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa 
     1741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc 
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg 
     1861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg 
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa 
     1981 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt 
     2041 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg 
     2101 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat 
     2161 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct 
     2221 agctatagtt ctagtggttg gctacgtata ctccggaata ttaatagatc atggagataa 
     2281 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt 
     2341 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc 
     2401 tcggtccgaa aaccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt 
     2461 tccagggcca tcgaacaacg agaatcaaga tcactgagct aaatccccac ctgatgtgtg 
     2521 tgctttgtgg agggtacttc attgatgcca caaccataat agaatgtcta cattccttct 
     2581 gtaaaacgtg tattgttcgt tacctggaga caagcaagta ttgtcctatt tgtgatgtcc 
     2641 aagttcacaa gaccagacca ctactgaata taaggtcaga taaaactctc caagatattg 
     2701 tatacaaatt agttccaggg cttttcaaaa atgaaatgaa gagaagaagg gatttttatg 
     2761 cagctcatcc ttctgctgat gctgccaatg gctctaatga agatagagga gaggttgcag 
     2821 atgaagataa gagaattata actgatgatg agataataag cttatccatt gaattctttg 
     2881 accagaacag attggatcgg aaagtaaaca aagacaaaga gaaatctaag gaggaggtga 
     2941 atgataaaag atacttacga tgcccagcag caatgactgt gatgcactta agaaagtttc 
     3001 tcagaagtaa aatggacata cctaatactt tccagattga tgtcatgtat gaggaggaac 
     3061 ctttaaagga ttattataca ctaatggata ttgcctacat ttatacctgg agaaggaatg 
     3121 gtccacttcc attgaaatac agagttcgac ctacttgtaa aagaatgaag atcagtcacc 
     3181 agagagatgg actgacaaat gctggagaac tggaaagtga ctctgggagt gacaaggcca 
     3241 acagcccagc aggaggtatt ccctccacct cttcttgttt gcctagcccc agtactccag 
     3301 tgcagtctcc tcatccacag tttcctcaca tttccagtac tatgaatgga accagcaaca 
     3361 gccccagcgg taaccaccaa tcttcttttg ccaatagacc tcgaaaatca tcagtaaatg 
     3421 ggtcatcagc aacttcttct ggttgatgac gaagcttgtc gagaagtact agaggatcat 
     3481 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 
     3541 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 
     3601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     3661 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc 
     3721 actgatatcg cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt 
     3781 catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact 
     3841 cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca actattttgt 
     3901 ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc 
     3961 catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa aatttttctg 
     4021 tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt tgcagcctga 
     4081 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     4141 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     4201 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     4261 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     4321 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     4381 tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     4441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     4501 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     4561 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     4621 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     4681 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     4741 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     4801 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     4861 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     4921 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     4981 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     5041 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     5101 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     5161 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     5221 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     5281 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     5341 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     5401 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     5461 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     5521 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     5581 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     5641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     5701 ggatcttc
//