LOCUS       PFBOH_RNF2              5738 bp    DNA     circular     07-Aug-2024
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     mobile_element     894..1118
                     /label="Tn7R"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     mobile_element     3774..3939
                     /label="Tn7L"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     polyA_signal     3611..3745
                     /label="SV40 poly(A) signal"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#cc99ff"
     promoter        4603..4707
                     /label="AmpR promoter"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        2268..2359
                     /label="polyhedrin promoter"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             4708..5568
                     /label="AmpR"
                     /ApEinfo_fwdcolor="#ffc800"
     rep_origin      1..589
                     /label="ori"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     CDS             complement(1185..1718)
                     /label="GmR"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             2469..3473
                     /label="RNF2"
                     /ApEinfo_fwdcolor="#ffc800"
     rep_origin      4123..4577
                     /label="f1 ori"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     promoter        1888..1916
                     /label="Pc promoter"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     775..826
                     /label="bom (fragment)"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     CDS             complement(1894..2241)
                     /label="YPR2_ECOLX (fragment)"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             3505..3588
                     /label="large T antigen (fragment)"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             4102..4118
                     /label="lacZ? (fragment)"
                     /ApEinfo_fwdcolor="#ffc800"
     CDS             2415..2435
                     /label="Feature 17"
                     /note="6x his tag"
                     /ApEinfo_fwdcolor="#c8c8ff"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa 
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc 
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac 
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac 
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa 
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg 
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt 
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact 
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc 
     1321 tcatgcttga gcagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag 
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa 
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta 
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg 
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg 
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga 
     1681 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa 
     1741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc 
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg 
     1861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg 
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa 
     1981 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt 
     2041 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg 
     2101 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat 
     2161 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct 
     2221 agctatagtt ctagtggttg gctacgtata ctccggaata ttaatagatc atggagataa 
     2281 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt 
     2341 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc 
     2401 tcggtccgaa aaccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt 
     2461 tccagggctc tcaggctgtg cagacaaacg gaactcaacc attaagcaaa acatgggaac 
     2521 tcagtttata tgagttacaa cgaacacctc aggaggcaat aacagatggc ttagaaattg 
     2581 tggtttcacc tcgaagtcta cacagtgaat taatgtgccc aatttgtttg gatatgttga 
     2641 agaacaccat gactacaaag gagtgtttac atcgtttttg tgcagactgc atcatcacag 
     2701 cccttagaag tggcaacaaa gaatgtccta cctgtcggaa aaaactagtt tccaaaagat 
     2761 cactaaggcc agacccaaac tttgatgcac tcatcagcaa aatttatcca agtcgtgatg 
     2821 agtatgaagc tcatcaagag agagtattag ccaggatcaa caagcacaat aatcagcaag 
     2881 cactcagtca cagcattgag gaaggactga agatacaggc catgaacaga ctgcagcgag 
     2941 gcaagaaaca acagattgaa aatggtagtg gagcagaaga taatggtgac agttcacact 
     3001 gcagtaatgc atccacacat agcaatcagg aagcaggccc tagtaacaaa cggaccaaaa 
     3061 catctgatga ttctgggcta gagcttgata ataacaatgc agcaatggca attgatccag 
     3121 taatggatgg tgctagtgaa attgaattag tattcaggcc tcatcccaca cttatggaaa 
     3181 aagatgacag tgcacagacg agatacataa agacttctgg taacgccact gttgatcact 
     3241 tatccaagta tctggctgtg aggttagctt tagaagaact tcgaagcaaa ggtgaatcaa 
     3301 accagatgaa ccttgataca gccagtgaga agcagtatac catttatata gcaacagcca 
     3361 gtggccagtt cactgtatta aatggctctt tttctttgga attggtcagt gagaaatact 
     3421 ggaaagtgaa caaacccatg gaactttatt acgcacctac aaaggagcac aaatgatgac 
     3481 gaagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt 
     3541 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
     3601 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     3661 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     3721 caatgtatct tatcatgtct ggatctgatc actgatatcg cctaggagat ccgaaccaga 
     3781 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg 
     3841 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt 
     3901 tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt 
     3961 tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt 
     4021 tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg 
     4081 actgaatatc aacgcttatt tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 
     4141 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     4201 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     4261 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     4321 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     4381 tttttcgccc tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg 
     4441 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     4501 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     4561 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     4621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     4681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     4741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     4801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     4861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     4921 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     4981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     5041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     5101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     5161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     5221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     5281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     5341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     5401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     5461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     5521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     5581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     5641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     5701 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//