LOCUS       Exported               11851 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUt-MONARCH 1.0_crEGFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Yiming Wan
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, July 25, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     intron          107..679
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             697..1320
                     /codon_start=1
                     /note="hTetR"
                     /translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGG"
     CDS             1321..1338
                     /note="NLS"
     CDS             1348..1404
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1405..2112
                     /locus_tag="mCherry"
                     /label=mCherry
                     /note="mCherry"
     misc_feature    2122..2178
                     /locus_tag="P2A"
                     /label=P2A
                     /note="P2A"
     misc_feature    2176..2181
                     /locus_tag="XmaI"
                     /label=XmaI
                     /note="XmaI"
     misc_feature    2182..5082
                     /locus_tag="Rfx Cas13d"
                     /label=Rfx Cas13d
                     /note="Rfx Cas13d"
     misc_feature    2896..2913
                     /locus_tag="HEPN1"
                     /label=HEPN1
                     /note="HEPN1"
     misc_feature    4753..4770
                     /locus_tag="HEPN2"
                     /label=HEPN2
                     /note="HEPN2"
     misc_feature    5083
                     /locus_tag="SpeI(1)"
                     /label=SpeI(1)
                     /note="SpeI(1)"
     misc_feature    5084..5088
                     /locus_tag="SpeI"
                     /label=SpeI
                     /note="SpeI"
     misc_feature    5089..5091
                     /locus_tag="linker from baohui(2)"
                     /label=linker from baohui(2)
                     /note="linker from baohui(2)"
     misc_feature    5092
                     /locus_tag="NLS"
                     /label=NLS
                     /note="NLS"
     misc_feature    5092
                     /locus_tag="linker from baohui(1)"
                     /label=linker from baohui(1)
                     /note="linker from baohui(1)"
     misc_feature    5093..5099
                     /locus_tag="NLS(1)"
                     /label=NLS(1)
                     /note="NLS(1)"
     misc_feature    5093..5099
                     /locus_tag="linker from baohui(3)"
                     /label=linker from baohui(3)
                     /note="linker from baohui(3)"
     misc_feature    5100..5106
                     /locus_tag="sv40 NLS"
                     /label=sv40 NLS
                     /note="sv40 NLS"
     misc_feature    5100..5106
                     /locus_tag="linker from baohui"
                     /label=linker from baohui
                     /note="linker from baohui"
     misc_feature    5107..5115
                     /locus_tag="linker from baohui(4)"
                     /label=linker from baohui(4)
                     /note="linker from baohui(4)"
     misc_feature    5107..5112
                     /locus_tag="NLS(2)"
                     /label=NLS(2)
                     /note="NLS(2)"
     misc_feature    5116..5121
                     /locus_tag="linker from baohui(5)"
                     /label=linker from baohui(5)
                     /note="linker from baohui(5)"
     misc_feature    5122..5148
                     /locus_tag="c-Myc NLS"
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
     misc_feature    5149..5154
                     /locus_tag="BamHI"
                     /label=BamHI
                     /note="BamHI"
     misc_feature    5155..5167
                     /locus_tag="3x Flag Tag(3)"
                     /label=3x Flag Tag(3)
                     /note="3x Flag Tag(3)"
     misc_feature    5168..5186
                     /locus_tag="3x Flag Tag(2)"
                     /label=3x Flag Tag(2)
                     /note="3x Flag Tag(2)"
     misc_feature    5187..5211
                     /locus_tag="3x Flag Tag(1)"
                     /label=3x Flag Tag(1)
                     /note="3x Flag Tag(1)"
     misc_feature    5212..5220
                     /locus_tag="3x Flag Tag"
                     /label=3x Flag Tag
                     /note="3x Flag Tag"
     misc_feature    5224..5229
                     /locus_tag="BsiWI"
                     /label=BsiWI
                     /note="BsiWI"
     misc_feature    5249..5284
                     /locus_tag="RfxCas13d DR36"
                     /label=RfxCas13d DR36
                     /note="RfxCas13d DR36"
     misc_feature    5285..5307
                     /note="CrEGFP-1"
     misc_feature    5308..5343
                     /locus_tag="RfxCas13d DR36(1)"
                     /label=RfxCas13d DR36(1)
                     /note="RfxCas13d DR36(1)"
                     /note="RfxCas13d DR36"
     misc_feature    5396..5754
                     /note="WPRE"
     polyA_signal    5812..6036
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_site      6171..6565
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     gene            complement(6573..6996)
                     /product="Blasticidin Resistance gene"
                     /note="BsrS2"
     promoter        complement(7005..7302)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    7329..7347
                     /label=SA
                     /note="SA"
     misc_feature    complement(7348..7381)
                     /note="F3"
     intron          7382..7425
                     /label=HA1-AAVS1
                     /note="HA1-AAVS1"
     misc_feature    7388..7425
                     /note="HA-L"
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        7448..7552
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             7553..8413
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        8446..8942
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     5'UTR           8984..9023
                     /note="HSV TK 5'-UTR"
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             9043..10173
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /note="HSV TK"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    10215..10263
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10287..10875
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(10880..10915)
                     /note="FRT (minimal)"
     promoter        10933
                     /note="CMV-D2i Promoter"
     promoter        11152..11821
                     /note="CMV-D2i Promoter"
     enhancer        11152..11531
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     TATA_signal     11721..11727
     protein_bind    11731..11749
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     TATA_signal     11750..11756
                     /note="Initiator Element"
     protein_bind    11757..11775
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccggggatcc tctagggcct ctgagctatt ccagaagtag tgaagaggct tttttggagg
       61 cctaggcttt tgcaaaaagc tccggatcga tcctgagaac ttcagggtga gtttggggac
      121 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
      181 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
      241 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
      301 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
      361 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
      421 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
      481 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
      541 caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
      601 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
      661 cttcttcttt ttcctacagg tcctgcaggc gccaccatgg gttctagact ggacaagagc
      721 aaagtcataa actctgctct ggaattactc aatgaagtcg gtatcgaagg cctgacgaca
      781 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
      841 aagcgggccc tgctcgatgc cctggcaatc gagatgctgg acaggcatca tacccacttc
      901 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc
      961 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     1021 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     1081 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     1141 gatcaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     1201 ccacttctga gacaagcaat tgagctgttc gaccatcagg gagccgaacc tgccttcctt
     1261 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     1321 ccaaaaaaga agagaaaggg aagcggagct actaacttca gcctgctgaa gcaggctgga
     1381 gacgtggagg agaaccctgg acctatggtg agcaagggcg aggaggataa catggccatc
     1441 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1501 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1561 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1621 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1681 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1741 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1801 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1861 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1921 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1981 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     2041 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     2101 gagctgtaca agggcagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     2161 gaggagaacc ctggacccgg gatgatcgag aaaaagaagt ccttcgctaa gggcatgggc
     2221 gtgaagtcca cactcgtgtc cggctccaaa gtgtacatga caaccttcgc cgaaggcagc
     2281 gacgccaggc tggaaaagat cgtggagggc gacagcatca ggagcgtgaa tgagggcgag
     2341 gccttcagcg ctgaaatggc cgataaaaac gccggctata agatcggcaa cgccaaattc
     2401 agccatccta agggctacgc cgtggtggct aacaaccctc tgtatacagg acccgtccag
     2461 caggatatgc tcggcctgaa ggaaactctg gaaaagaggt acttcggcga gagcgctgat
     2521 ggcaatgaca atatttgtat ccaggtgatc cataacatcc tggacattga aaaaatcctc
     2581 gccgaataca ttaccaacgc cgcctacgcc gtcaacaata tctccggcct ggataaggac
     2641 attattggat tcggcaagtt ctccacagtg tatacctacg acgaattcaa agaccccgag
     2701 caccataggg ccgctttcaa caataacgat aagctcatca acgccatcaa ggcccagtat
     2761 gacgagttcg acaacttcct cgataacccc agactcggct atttcggcca ggcctttttc
     2821 agcaaggagg gcagaaatta catcatcaat tacggcaacg aatgctatga cattctggcc
     2881 ctcctgagcg gactgagaca ctgggtggtc cacaacaacg aagaagagtc caggatctcc
     2941 aggacctggc tctacaacct cgataagaac ctcgacaacg aatacatctc caccctcaac
     3001 tacctctacg acaggatcac caatgagctg accaactcct tctccaagaa ctccgccgcc
     3061 aacgtgaact atattgccga aactctggga atcaaccctg ccgaattcgc cgaacaatat
     3121 ttcagattca gcattatgaa agagcagaaa aacctcggat tcaatatcac caagctcagg
     3181 gaagtgatgc tggacaggaa ggatatgtcc gagatcagga aaaatcataa ggtgttcgac
     3241 tccatcagga ccaaggtcta caccatgatg gactttgtga tttataggta ttacatcgaa
     3301 gaggatgcca aggtggctgc cgccaataag tccctccccg ataatgagaa gtccctgagc
     3361 gagaaggata tctttgtgat taacctgagg ggctccttca acgacgacca gaaggatgcc
     3421 ctctactacg atgaagctaa tagaatttgg agaaagctcg aaaatatcat gcacaacatc
     3481 aaggaattta ggggaaacaa gacaagagag tataagaaga aggacgcccc tagactgccc
     3541 agaatcctgc ccgctggccg tgatgtttcc gccttcagca aactcatgta tgccctgaca
     3601 atgttcctgg atggcaagga gatcaacgac ctcctgacca ccctgattaa taaattcgat
     3661 aacatccaga gcttcctgaa ggtgatgcct ctcatcggag tcaacgctaa gttcgtggag
     3721 gaatacgcct ttttcaaaga ctccgccaag atcgccgatg agctgaggct gatcaagtcc
     3781 ttcgctagaa tgggagaacc tattgccgat gccaggaggg ccatgtatat cgacgccatc
     3841 cgtattttag gaaccaacct gtcctatgat gagctcaagg ccctcgccga caccttttcc
     3901 ctggacgaga acggaaacaa gctcaagaaa ggcaagcacg gcatgagaaa tttcattatt
     3961 aataacgtga tcagcaataa aaggttccac tacctgatca gatacggtga tcctgcccac
     4021 ctccatgaga tcgccaaaaa cgaggccgtg gtgaagttcg tgctcggcag gatcgctgac
     4081 atccagaaaa aacagggcca gaacggcaag aaccagatcg acaggtacta cgaaacttgt
     4141 atcggaaagg ataagggcaa gagcgtgagc gaaaaggtgg acgctctcac aaagatcatc
     4201 accggaatga actacgacca attcgacaag aaaaggagcg tcattgagga caccggcagg
     4261 gaaaacgccg agagggagaa gtttaaaaag atcatcagcc tgtacctcac cgtgatctac
     4321 cacatcctca agaatattgt caatatcaac gccaggtacg tcatcggatt ccattgcgtc
     4381 gagcgtgatg ctcaactgta caaggagaaa ggctacgaca tcaatctcaa gaaactggaa
     4441 gagaagggat tcagctccgt caccaagctc tgcgctggca ttgatgaaac tgcccccgat
     4501 aagagaaagg acgtggaaaa ggagatggct gaaagagcca aggagagcat tgacagcctc
     4561 gagagcgcca accccaagct gtatgccaat tacatcaaat acagcgacga gaagaaagcc
     4621 gaggagttca ccaggcagat taacagggag aaggccaaaa ccgccctgaa cgcctacctg
     4681 aggaacacca agtggaatgt gatcatcagg gaggacctcc tgagaattga caacaagaca
     4741 tgtaccctgt tcaggaacaa ggccgtccat ctggaagtgg ccaggtatgt ccacgcctat
     4801 atcaacgaca ttgccgaggt caattcctac ttccaactgt accattacat catgcagaga
     4861 attatcatga atgagaggta cgagaaaagc agcggaaagg tgtccgagta cttcgacgct
     4921 gtgaatgacg agaagaagta caacgatagg ctcctgaaac tgctgtgtgt gcctttcggc
     4981 tactgtatcc ccaggtttaa gaacctgagc atcgaggccc tgttcgatag gaacgaggcc
     5041 gccaagttcg acaaggagaa aaagaaagtg tccggcaatt ccactagtgc tcccaagaaa
     5101 aagcgcaagg taggtggaag cccagcagct aaaagagtta aattggatgg atccgattat
     5161 aaggatcacg atggagatta caaggaccac gacatagact acaaagatga tgacgacaag
     5221 taacgtacgg ccgcgactct agagtcgaca agtaaacccc taccaactgg tcggggtttg
     5281 aaaccatgat atagacgttg tggctgtcaa gtaaacccct accaactggt cggggtttga
     5341 aactcgacct gcaggcatgc aagcttgata tcaggccgca atcaacctct ggattacaaa
     5401 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5461 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5521 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5581 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5641 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5701 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5761 gtgttgtcct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5821 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5881 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5941 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6001 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6061 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6121 tggttacgcg ctaaagcaag taaaacctct acaaatgtgg tatggaattc taaaatacag
     6181 catagcaaaa ctttaacctc caaatcaagc ctctacttga atccttttct gagggatgaa
     6241 taaggcatag gcatcagggg ctgttgccaa tgtgcattag ctgtttgcag cctcaccttc
     6301 tttcatggag tttaagatat agtgtatttt cccaaggttt gaactagctc ttcatttctt
     6361 tatgttttaa atgcactgac ctcccacatt ccctttttag taaaatattc agaaataatt
     6421 taaatacatc attgcaatga aaataaatgt tttttattag gcagaatcca gatgctcaag
     6481 gcccttcata atatccccca gtttagtagt tggacttagg gaacaaagga acctttaata
     6541 gaaattggac agcaagaaag cgagcttcta gctttagttc ctggtgtact tgagggggat
     6601 gagttcctca atggtggttt tgaccagctt gccattcatc tcaatgagca caaagcagtc
     6661 aggagcatag tcagagatga gctctctgca catgccacag gggctgacca ccctgatgga
     6721 tctgtccacc tcatcagagt aggggtgcct gacagccaca atggtgtcaa agtccttctg
     6781 cccgttgctc acagcagacc caatggcaat ggcttcagca cagacagtga ccctgccaat
     6841 gtaggcctca atgtggacag cagagatgat ctccccagtc ttggtcctga tggccgcccc
     6901 gacatggtgc ttgttgtcct catagagcat ggtgatcttc tcagtggcga cctccaccag
     6961 ctccagatcc tgctgagaga tgttgaaggt cttcatggta gcctccaaaa aagcctcctc
     7021 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     7081 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     7141 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     7201 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     7261 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     7321 acagctgcga agagaagagg tcaggccgaa gttcctatac tatttgaaga ataggaactt
     7381 caagcttgcc ccactgtggg gtggagggga cagataaaag taccctggca cttttcgggg
     7441 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7501 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7561 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7621 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7681 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7741 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7801 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7861 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7921 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7981 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8041 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8101 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8161 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8221 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8281 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8341 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8401 taagcattgg taactgtcag accaagttta ttctacggtt ctaccgggta ggggaggcgc
     8461 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     8521 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     8581 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     8641 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     8701 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     8761 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     8821 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     8881 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     8941 tttcgacctg cagcgacccg ctaacagcgt caacagcgtg ccgcagatct tggtggcgtg
     9001 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc gtacccctgc
     9061 catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag caaccgacgt
     9121 acggcgttgc gccctcgccg gcagcaagaa gccacggaag tccgcctgga gcagaaaatg
     9181 cccacgctac tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg
     9241 caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact
     9301 tactggcagg tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc
     9361 ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata
     9421 acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggggag
     9481 gctgggagct cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc
     9541 gccgccctcc tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc
     9601 gtgctggcgt tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg
     9661 gcccttccgg aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg
     9721 cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg
     9781 cggtatctgc agggcggcgg gtcgtggcgg gaggattggg gacagctttc ggggacggcc
     9841 gtgccgcccc agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac
     9901 acgttattta ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtacaac
     9961 gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc
    10021 ctggattacg accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg
    10081 atggtccaga cccacgtcac cacccccggc tccataccga cgatctgcga cctggcgcgc
    10141 acgtttgccc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    10201 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
    10261 ttcataaacg cgctcatagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10861 cggagcctat ggaaaaacgc cgaagttcct atactttcta gagaatagga acttcacggg
    10921 ccagatatac gcgacgggcc agatatacgc gacgggccag atatacgcga cgggccagat
    10981 atacgcgacg ggccagatat acgcgacggg ccagatatac gcgacgggcc agatatacgc
    11041 gacgggccag atatacgcga cgggccagat atacgcgacg ggccagatat acgcgacggg
    11101 ccagatatac gcgacgggcc agatatacgc gacgggccag atatacgcgt tgacattgat
    11161 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    11221 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    11281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    11341 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    11401 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    11461 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    11521 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
    11581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    11641 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    11701 ggcgtgtacg gtgggaggtc tatataagca tccctatcag tgatagagat cagatctccc
    11761 tatcagtgat agagagctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
    11821 ggactctagc gtttaaactt aagcttggta c
//
