LOCUS       pUBC(k)-AT5G0164       11522 bp DNA     circular SYN 29-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..639
                     /label=pUBQ10
                     /note="/vntifkey=29"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     669..676
                     /label=attR1
                     /note="/vntifkey=86"
     misc_feature    677..693
                     /label=attL1
     protein_bind    complement(1369..1393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1369..1378
                     /label=attL2
     misc_recomb     complement(1379..1393)
                     /label=attR2
                     /note="/vntifkey=86"
     misc_feature    1440..2111
                     /label=mKOk
     terminator      2130..2338
                     /label=T35S
                     /note="/vntifkey=43"
     promoter        2660..2843
                     /function="NOS promoter"
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
     CDS             2844..3665
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of 
                     nopalinesynthase and Tn5 aminoglycoside phosphotransferase"
                     /label=nos-nptII
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4290..4514
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             4515..4561
                     /label=BarR
                     /note="/vntifkey=4"
     misc_feature    complement(4565..4897)
                     /label=LB
                     /note="/vntifkey=21"
     CDS             4961..5971
                     /label=SmR
                     /note="/vntifkey=4"
     rep_origin      6422..7102
                     /label=pBR322
                     /note="/vntifkey=33"
     rep_origin      7512..10105
                     /label=pVS1
                     /note="/vntifkey=33"
     misc_feature    11082..11230
                     /label=RB
                     /note="/vntifkey=21"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggtgtcaaca aatccccctg
      721 ttcttccaat ttccaccacc gcaacagaca ccaccaatca gcctccgatt gtaaccgctg
      781 tggtggaatc gcaacctcca gtcgtccgtg catttgtcaa cggtgtgacc gagacggtct
      841 gtggtggtct atctcgtagc cgtccgtggt cagagctcct cgatcgatcc gctttcacaa
      901 agcctgactc tctctccgag gccgggacgc gattccggaa aaattcctct tatttcaggg
      961 tgaattacgt ttgcattgtc gctctgatcc ttggtttctc tcttctcgcc cacccattct
     1021 ctctcatcct cctcctatgt ctcgccgctt cctggctctt cctctacctc ttccgtccat
     1081 cagatcgtcc tcttatcctc ttcggccgat ctttctccga atacgaaact ctcggtggcc
     1141 taatcctctc cacgatcgca gtgatcttct tcacaagcgt cggatctgta ttgatatccg
     1201 ctctcatgat cggaatagca acaatctgtg tccatggcgc ctttagagct cccgatgatc
     1261 tcttcctcga cgaacaagat catgccgcct ccggattcct ctcattcatc ggtgttcccg
     1321 cgatcccctc tgtagcaccc tctgcctcct cagcggcttc ccccgtgcac ccagctttct
     1381 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     1441 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     1501 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     1561 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     1621 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     1681 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     1741 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     1801 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     1861 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     1921 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     1981 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     2041 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     2101 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2161 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2221 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2281 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     2341 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     2401 gcactagtga tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc
     2461 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2521 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2581 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2641 ggaaattgta aacgttaatg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     2701 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     2761 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     2821 actctcaatc caaataatct gcaatggcaa ttaccttatc cgcaacttct ttacctattt
     2881 ccgcccggat ccgggcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     3421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     3481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     3541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3661 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     3841 tccaacactt acgtttgcaa cgtccaagag caaatagacc acgaacgccg gaaggttgcc
     3901 gcagcgtgtg gattgcgtct caattctctc ttgcaggaat gcaatgatga atatgatact
     3961 gactatgaaa ctttgaggga atactgccta gcaccgtcac ctcataacgt gcatcatgca
     4021 tgccctgaca acatggaaca tcgctatttt tctgaagaat tatgctcgtt ggaggatgtc
     4081 gcggcaattg cagctattgc caacatcgaa ctacccctca cgcatgcatt catcaatatt
     4141 attcatgcgg ggaaaggcaa gattaatcca actggcaaat catccagcgt gattggtaac
     4201 ttcagttcca gcgacttgat tcgttttggt gctacccacg ttttcaataa ggacgagatg
     4261 gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa taaagtttct
     4321 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4381 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4441 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4501 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     4561 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     4621 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     4681 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     4741 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     4801 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     4861 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     4921 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     4981 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5041 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5101 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5161 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5221 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5281 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     5341 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     5401 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     5461 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     5521 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     5581 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     5641 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     5701 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5761 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5821 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     5881 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     5941 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6001 gccgcttcgc ggcgcggctt aactcaagtc gttagatgca ctaagcacat aattgctcac
     6061 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     6121 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     6181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6361 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6961 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     7021 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     7081 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     7141 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7201 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7261 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     7321 gtccgcgccc tggtagattg cctggcccta ggccagccat ttttgagcgg ccagcggccg
     7381 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     7441 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     7501 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     7561 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     7621 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     7681 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     7741 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     7801 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     7861 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     7921 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     7981 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     8041 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     8101 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     8161 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     8221 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     8281 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     8341 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     8401 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     8461 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     8521 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     8581 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
     8641 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     8701 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     8761 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc cgattcctcg
     8821 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     8881 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8941 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9001 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9061 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9121 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9181 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9241 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9301 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9361 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9421 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9481 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9541 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9601 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9661 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9721 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9781 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9841 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9901 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9961 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10021 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10081 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10141 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10201 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10261 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10321 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10381 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10441 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10501 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10561 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10621 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10681 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10741 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10801 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10861 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    10921 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    10981 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11041 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    11101 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11161 tatccgttat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    11221 aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc
    11281 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    11341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11401 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11461 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgagctc
    11521 gg
//
