LOCUS       VB231211-1031fds        6641 bp    DNA     circular SYN 29-OCT-2024
DEFINITION  
ACCESSION   VB231211-1031fds
VERSION     VB231211-1031fds
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..6641
     ITR             1..130
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional
                     equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /name="5' ITR"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="6d343a478711450d8e5c28ceb27e5b84"
     Promoter        158..1336
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Strong promoter."
                     /full_name="Human eukaryotic translation elongation factor
                     1 alpha1 promoter"
                     /label="{EF1A}"
                     /name="{EF1A}"
                     /note="color: #da3bb5; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="58350a0845b9413da66b454a715c2ef1"
     protein_bind    1390..1423
                     /__SeqFeature__="True"
                     /__level__="0"
                     /label="FRT (minimal)"
                     /name="FRT (minimal)"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="FRT (minimal)"
                     /uuid="6a7783e08a0e460dab0020bf416197b2"
     misc_feature    1474..1507
                     /__SeqFeature__="True"
                     /__level__="0"
                     /label="frt*"
                     /name="frt*"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="frt*"
                     /uuid="0608a47d1d204a7b82f5f07ef6b12867"
     misc_feature    complement(1519..2757)
                     /__SeqFeature__="True"
                     /__level__="0"
                     /label="OCAMP"
                     /name="OCAMP"
                     /note="color: #05696d; direction: LEFT"
                     /official_designation="OCAMP"
                     /uuid="66e3fc5628334f14b0d620138ea2745c"
     misc_feature    2773..2796
                     /__SeqFeature__="True"
                     /__level__="0"
                     /direction=NONE
                     /label="Xpress™ tag"
                     /name="Xpress™ tag"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="Xpress™ tag"
                     /uuid="c3d621ede391409380f7d73be893164c"
     misc_feature    2800..2832
                     /__SeqFeature__="True"
                     /__level__="0"
                     /direction=NONE
                     /label="T7 tag (gene 10 leader)"
                     /name="T7 tag (gene 10 leader)"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="T7 tag (gene 10 leader)"
                     /uuid="bcab9ebd98c34283a0b213367e9adc57"
     misc_feature    2836..2853
                     /__SeqFeature__="True"
                     /__level__="0"
                     /direction=NONE
                     /label="6xHis"
                     /name="6xHis"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="6xHis"
                     /uuid="7ec35cb763e2479fb6ce4fa54e98abd4"
     misc_feature    2866..2871
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /direction=NONE
                     /full_name="Kozak"
                     /label="Kozak"
                     /name="Kozak"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="ba4f837cfff042e38e7561244b24cfa6"
     protein_bind    complement(2878..2911)
                     /__SeqFeature__="True"
                     /__level__="0"
                     /label="FRT (minimal)"
                     /name="FRT (minimal)"
                     /note="color: #0375bb; direction: LEFT"
                     /official_designation="FRT (minimal)"
                     /uuid="bc34a8c998a24ea89003a774e3890813"
     misc_feature    complement(2962..2995)
                     /__SeqFeature__="True"
                     /__level__="0"
                     /label="frt*"
                     /name="frt*"
                     /note="color: #a949ca; direction: LEFT"
                     /official_designation="frt*"
                     /uuid="0109dfc9d9004982897318c377c7ac1d"
     misc_feature    3050..3647
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /name="WPRE"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     polyA_signal    3678..3885
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /name="BGH pA"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3893..4022)
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /name="3' ITR"
                     /note="color: #d84e4e; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             4950..5810
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Allows E. coli to be resistant to
                     ampicillin."
                     /full_name="Ampicillin resistance gene"
                     /label="Ampicillin"
                     /name="Ampicillin"
                     /note="color: #1f36a9; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     rep_origin      5981..6569
                     /__SeqFeature__="True"
                     /__level__="0"
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /name="pUC ori"
                     /note="color: #db6901; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgggc tccggtgccc gtcagtgggc
      181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      361 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      421 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      481 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      541 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      601 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      661 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      721 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      781 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      841 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      901 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      961 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1021 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1081 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1141 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1201 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1261 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1321 atttcaggtg tcgtgacaag tttgtacaaa aaagcaggct gtaccggatc ctctagagtc
     1381 gactccggag aagttcctat tctctagaaa gtataggaac ttcgcagaat ggtagctgga
     1441 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattcttca aaaggtatag
     1501 gaacttcggc gcgccttatt acttcgctgt catcatttgt acaaactctt cgtagtttac
     1561 ctgaccatcc ccatcgatgt ctgctaccct gatcatttca tcaacctcct catctgttaa
     1621 cttctctcca aggtctgtca tcacgtggcg aagctctgct gcgccgatgt agccattgcc
     1681 gtccttatca aacacgcgga acgcttctct aatttcttct tcactgtctg tgtcattcat
     1741 ctttcttgcc atcatcgtca ggaactcagg gaagtcgaat gtgccgtcac cgtcggcatc
     1801 tacttcattg atcatgtcct gcagctctgc ttctgtgggg ttctgcccca gagaccggaa
     1861 caccgtcccc agctccttgg ttgttatcgt cccatccccg tccttgtcaa atagggagaa
     1921 agcctcttta aattctgcga tctgctcttc agtcagttgg tcacgcgtag cctcccagcc
     1981 catggtcttc ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcagcttcac
     2041 cttgtagatg aatacgccgt cctgcaggga ggagtcctgg ttaacgtgaa taatgccgcc
     2101 gtcctcgaag ttcatcacgc gctcccacct gaagccctcg gggaaggaca gcttaaagta
     2161 gtcggggatg tcggctgggt gcttaatgta ggccttggag ccgtatgtga actgagggga
     2221 caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct tagcggtctg
     2281 aaaggcctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
     2341 ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc
     2401 ctcgcccttg ctcaccagac tccctcctgt acctcccttg tacagctcgt ccctgccgcc
     2461 ggtggggtgg cggccctcgg cgcgttcgca ctgttccacg atggtgtagt cctcgttgtg
     2521 ggacacgatg tccaacttta tgtcgacgat gtaggcgccg ggcagctgca cgggcttctt
     2581 ggccttgtag gtggtcttga cctcggcggc gtagtggccg ccgtccttca gcctcagccc
     2641 cttcttgatc tcgctcttca ggacgccgtc ctcggggtac atccgctcgg aaaccacggg
     2701 tgagctcagc cgacctatag ctctgactgc gtgacctgcc ttgttccact tacgacggga
     2761 tccccatcga tccttatcgt catcgtcgta cagatcccga cccatttgct gtccaccagt
     2821 catgctagcc ataccatgat gatgatgatg atgagaaccc cgcatggtgg cgctagcgaa
     2881 gttcctatac tttctagaga ataggaactt cttgccttaa cccagaaatt atcactgtta
     2941 ttctttagaa tggtgcaaag agaagttcct ataccttttg aagaatagga acttcgaatt
     3001 cgatatcaag cttatcgata cccagctttc ttgtacaaag tgggaattcc gataatcaac
     3061 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3421 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcctagagc
     3661 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3721 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3781 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3841 cagcaagggg gaggattggg aagagaatag caggcatgct ggggagggcc gcaggaaccc
     3901 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3961 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4021 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4081 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4321 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     4441 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     4501 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4561 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4621 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4681 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4741 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5641 ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg
     5701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt cctgcaggca g
//
