LOCUS       pBD15:_PB-TRE-ETV2      9104 bp ds-DNA     circular     09-JUL-2021
DEFINITION  .
KEYWORDS    "marker:AmpR, HygR" "ref:pmid\:25730490"
COMMENT     SP-dCas9-VPR with doxycycline-inducible expression. <small>[1]
            Highly efficient Cas9-mediated transcriptional programming. Nat
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. Chavez A, Scheiman J,
            Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD,
            Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE,
            Perrimon N, Weiss R, Aach J, Collins JJ, Church GM
            (pmid:25730490)</small>
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(131..586)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          561..582
                     /label="BD84: pCDNA3.1 Seq 3"
                     /note="sequence: GCTTAATGCGCCGCTACAGGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     728..744
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     repeat_region   874..936
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        1386..1764
                     /label="TRE3G promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    1394..1412
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1430..1448
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1466..1484
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1502..1520
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1538..1556
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1574..1592
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    1610..1628
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer          1648..1667
                     /label="BD69: pBD14 Sequencing One"
                     /note="sequence: GTACGGTGGGCGCCTATAAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1747..1766
                     /label="BD157: ETV2 Amplification FW"
                     /note="sequence: ACTTCCTACCCTCGTAAAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1747..2896
                     /label="Ordered Synthesized Sequence"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1791..1800
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1791..2829
                     /label="Translation 1791-2829"
                     /translation="ATMDLWNWDEASPQEVPPGNKLAGLEGAKLGFCFPDLALQGDTPTATAETCWKGTSSSLASFPQLDWGSALLHPEVPWGAEPDSQALPWSGDWTDMACTAWDSWSGASQTLGPAPLGPGPIPAAGSEGAAGQNCVPVAGEATSWSRAQAAGSNTSWDCSVGPDGDTYWGSGLGGEPRTDCTISWGGPAGPDCTTSWNPGLHAGGTTSLKRYQSSALTVCSEPSPQSDRASLARCPKTNHRGPIQLWQFLLELLHDGARSSCIRWTGNSREFQLCDPKEVARLWGERKRKPGMNYEKLSRGLRYYYRRDIVRKSGGRKYTYRFGGRVPSLAYPDCAGGGRGAETQ*K"
     CDS             1797..2825
                     /label="ETV2 Splice Isoform 2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2058..2077
                     /label="BD72: ETV2 Splice 2 Codon Op qPC"
                     /note="sequence: TCCGGAGACTGGACAGACAT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2187..2206)
                     /label="BD73: ETV2 Splice 2 Codon Op qPC"
                     /note="sequence: CCCGCTACAGGAACACAGTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2657..2676
                     /label="BD74: ETV2 Splice 2 Codon Op qPC"
                     /note="sequence: GCCCGGCATGAACTATGAGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2772..2791)
                     /label="BD75: ETV2 Splice 2 Codon Op qPC"
                     /note="sequence: CAGTCAGGATACGCAAGGCT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2875..2894
                     /label="BD76: KRAB Amplification FW"
                     /note="sequence: ACTGAAACACGGAAGGAGAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2877..2896)
                     /label="BD158: ETV2 Amplification RV"
                     /note="sequence: TTGTCTCCTTCCGTGTTTCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    2921..2969
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2995..3014)
                     /label="BD70: pBD14 Sequencing Two"
                     /note="sequence: gggtatcgacagagtgccag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        3200..3411
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             3424..3692
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3726..4469
                     /label="Tet-On® 3G"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4470..4523
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4545..5564
                     /label="HygR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    5675..5796
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   6558..6592
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7083..7099)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    7107..7123
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(7131..7137)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7138..7155)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7156..7161)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    7176..7197
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(7485..8073)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(8244..9035)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(9036..9104)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtctttcgt cttcaagaat
     1381 tcctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt
     1441 gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta
     1501 ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt
     1561 atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt
     1621 gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1681 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1741 cgtaccactt cctaccctcg taaaggtcta gagctagcga attcgaattt gccaccATGG
     1801 ACCTTTGGAA CTGGGATGAA GCAAGTCCGC AAGAAGTACC ACCTGGGAAC AAGCTGGCGG
     1861 GGCTGGAAGG AGCCAAACTC GGGTTTTGCT TCCCGGATCT TGCACTCCAG GGTGATACTC
     1921 CTACTGCAAC AGCCGAAACA TGTTGGAAAG GAACATCTTC TTCACTCGCC TCATTCCCTC
     1981 AGCTGGATTG GGGATCAGCA CTGCTGCATC CAGAAGTCCC ATGGGGCGCA GAGCCTGACT
     2041 CTCAGGCCCT GCCGTGGTCC GGAGACTGGA CAGACATGGC TTGCACCGCC TGGGACTCTT
     2101 GGTCCGGTGC ATCCCAAACG CTTGGTCCAG CGCCACTCGG TCCGGGTCCC ATTCCAGCTG
     2161 CTGGAAGTGA AGGGGCCGCA GGCCAGAACT GTGTTCCTGT AGCGGGCGAA GCTACGTCCT
     2221 GGTCCCGGGC ACAAGCAGCC GGTTCTAATA CCTCATGGGA TTGTAGCGTT GGCCCCGACG
     2281 GCGATACGTA CTGGGGCTCC GGGCTGGGGG GCGAACCTCG GACCGACTGT ACAATCAGTT
     2341 GGGGTGGGCC AGCTGGCCCA GATTGTACTA CTTCCTGGAA TCCCGGATTG CATGCGGGGG
     2401 GGACTACTTC ACTCAAGAGG TATCAAAGTT CCGCCCTGAC AGTGTGCAGC GAGCCCTCTC
     2461 CTCAATCAGA CCGAGCGTCT TTGGCTCGGT GCCCGAAGAC TAACCACAGG GGTCCCATAC
     2521 AACTGTGGCA ATTTTTGCTG GAATTGCTGC ACGACGGTGC TAGAAGTAGT TGCATACGGT
     2581 GGACTGGAAA CTCTAGGGAA TTTCAGTTGT GTGACCCCAA GGAAGTCGCA CGCCTTTGGG
     2641 GGGAACGAAA GAGAAAGCCC GGCATGAACT ATGAGAAGTT GAGTAGAGGT CTTCGGTACT
     2701 ATTATCGAAG AGACATTGTG CGGAAATCAG GAGGCAGGAA GTATACATAT AGATTTGGCG
     2761 GACGAGTGCC AAGCCTTGCG TATCCTGACT GTGCGGGAGG GGGGCGAGGT GCGGAAACTC
     2821 AGTAAaaggg ttcgatccct accggttagt aatgagttta aacgggggag gctaactgaa
     2881 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     2941 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     3001 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     3061 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     3121 aggccctgcc atagcagatc tgcgaaatcg gatccagcta aatctaagtg gatctgcgat
     3181 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3241 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     3421 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     3481 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     3541 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     3601 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     3661 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tactctagag
     3721 ccgccatgtc tagactggac aagagcaaag tcataaactc tgctctggaa ttactcaatg
     3781 gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     3841 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     3901 tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg caagactttc
     3961 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     4021 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     4081 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     4141 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     4201 agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag ctgttcgacc
     4261 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     4321 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     4381 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     4441 acgattttga ccttgacatg ctccccgggg agggcagagg aagtcttcta acatgcggtg
     4501 acgtggagga gaatcccggc cctgaattca tggatagatc cggaaagcct gaactcaccg
     4561 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     4621 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     4681 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     4741 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     4801 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     4861 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     4921 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     4981 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     5041 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     5101 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     5161 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     5221 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     5281 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     5341 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     5401 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     5461 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     5521 gtggaaaccg acgccccagc actcgtccga gggcaaagga ataggccaag gaacctctgg
     5581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5641 gtggatacgc tgctttaatg cctttgtatc agttaacttg tttattgcag cttataatgg
     5701 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5761 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg
     5821 atgtcaatta gtctatcaga agctatctgg tctcccttcc gggggacaag acatccctgt
     5881 ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc
     5941 aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag
     6001 gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc
     6061 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     6121 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     6181 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     6241 gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa
     6301 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     6361 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     6421 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     6481 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     6541 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
     6601 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     6661 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
     6721 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     6781 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     6841 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     6901 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     6961 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     7021 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     7081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7201 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7261 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7921 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcat
//