LOCUS       LS1004_Lamp_TEV        8517 bp DNA     circular SYN 15-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Stagi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1730..1739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1732..1885
                     /label=SP_mouseLAMP1
     misc_feature    1890..1958
                     /label=missing in Massi's other seq
     CDS             1894..2601
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2614..3321
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             3346..3366
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3367..4080
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4081..4101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4105..5256
                     /label=mouseLamp1
     CDS             5257..5280
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5296..5334
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             5350..5388
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     CDS             5404..5442
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
     primer_bind     complement(5535..5554)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5600..5655
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5654..5673)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6016..6032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6016..6032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6029..6051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6040..6056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6064..6070,6071..6088,6089..6094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6109..6130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6189..6384
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6235..6370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6297..6316
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6320..6331)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    6390..6524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6427..6446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6481..6500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6592..6609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6763..7351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6843..6862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7522..8313,8314..8382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8145..8164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8383..8487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcac catggcggcc
     1741 cccggcgccc ggcggccgct gctcctgctg ctgctggcag gccttgcaca tggcgcctca
     1801 gcagctagcg gtatggcggc ccccggcgcc cggcggccgc tgctcctgct gctgctggca
     1861 ggccttgcac atggcgcctc agcagctagc ggtatggtga gcaagggcga ggaggataac
     1921 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     1981 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2041 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2101 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2161 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2221 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2281 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2341 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2401 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2461 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2521 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2581 ggcatggacg agctgtacaa gggagctagc ggtatggtga gcaagggcga ggaggataac
     2641 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2701 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2761 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2821 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2881 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2941 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3001 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3061 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3121 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3181 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3241 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3301 ggcatggacg agctgtacaa gggagctagc ggggggcccg ggatggagaa tctgtatttc
     3361 caggggatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     3421 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     3481 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccttggcca
     3541 acacttgtca ctactttaac ttatggcgtt caatgctttt caagataccc agatcatatg
     3601 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     3661 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     3721 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     3781 cacaaattgg aatacaacta taacgatcac caggtgtaca tcatggcaga caaacaaaag
     3841 aatggaatca aagctaactt caaaattaga cacaacattg aagatggagg cgttcaacta
     3901 gcagaccatt atcaacaaaa tactccaatt ggcgatgggc ccgtcctttt accagacaac
     3961 cattacctgt ttacaacttc tactctttcg aaagatccca acgaaaagag agaccacatg
     4021 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     4081 gagaatctgt atttccaggg gcccgggggg ctctttgagg tgaaaaacaa tggcacaacg
     4141 tgtataatgg ccagcttctc tgcctccttt ctgaccacct acgagactgc gaatggttct
     4201 cagatcgtga acatttccct gccagcctct gcagaagtac tgaaaaatgg cagttcttgt
     4261 ggtaaagaaa atgtttctga ccccagcctc acaattactt ttggaagagg atatttactg
     4321 acactcaact tcacaaaaaa tacaacacgt tacagtgtcc agcatatgta ttttacatat
     4381 aacttgtcag atacagaaca ttttcccaat gccatcagca aagagatcta caccatggat
     4441 tccacaactg acatcaaggc agacatcaac aaagcatacc ggtgtgtcag tgatatccgg
     4501 gtctacatga agaatgtgac cgttgtgctc cgggatgcca ctatccaggc ctacctgtcg
     4561 agtggcaact tcagcaagga agagacacac tgcacacagg atggaccttc cccaaccact
     4621 gggccaccca gcccctcacc accacttgtg cccacaaacc ccactgtatc caagtacaat
     4681 gttactggta acaacggaac ctgcctgctg gcctctatgg cactgcaact gaatatcacc
     4741 tacctgaaaa aggacaacaa gacggtgacc agagcgttca acatcagccc aaatgacaca
     4801 tctagtggga gttgcggtat caacttggtg accctgaaag tggagaacaa gaacagagcc
     4861 ctggaattgc agtttgggat gaatgccagc tctagcctgt ttttcctgca aggagtgcgc
     4921 ttgaatatga ctcttcctga tgccctagtg cccacattca gcatctccaa ccattcactg
     4981 aaagctcttc aggccactgt gggaaactca tacaagtgca acactgagga acacatcttt
     5041 gtcagcaaga tgctctccct caatgtcttc agtgtgcagg tccaggcttt caaggtggac
     5101 agtgacaggt ttgggtctgt ggaagagtgt gttcaggatg gtaacaacat gttgatcccc
     5161 attgctgtgg gcggtgccct ggcagggctg gtcctcatcg tcctcattgc ctacctcatt
     5221 ggcaggaaga ggagtcacgc cggctatcag accatcttgg aggttctgtt tcaagggcct
     5281 gatatcgggg gaggttcaag actcgaagaa gagttgaggc gacggcttac ggaggatatc
     5341 ggtggtggat cccgactgga agaggagctc aggagaaggt tgactgagga tatcggaggg
     5401 ggttctcggc ttgaagaaga acttcgccga cgcctcacgg aagatatcta ggaattcact
     5461 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     5521 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     5581 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5641 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     5701 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     5761 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     5821 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     5881 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     5941 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     6001 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6061 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6181 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6301 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6361 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     6421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     6541 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7201 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7801 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtc
//
