LOCUS       Exported                8424 bp ds-DNA     circular SYN 11-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 29, 2022 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        1..138
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=Streptomycin/Spectinomycin adenyltransferase
                     /note="SpnR
                     vntifkey=4
                     ApEinfo_revcolor=pink
                     invitrogen=2750000
                     ApEinfo_fwdcolor=pink
                     dnas_title=SpnR
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="pUC origin
                     vntifkey=33
                     ApEinfo_revcolor=pink
                     invitrogen=2530000
                     ApEinfo_fwdcolor=pink
                     dnas_title=pUC origin
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     terminator      2319..2362
                     /note="rrnB T1 terminatior
                     vntifkey=43
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=rrnB T1 terminatior 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_recomb     2461..2560
                     /label=attL1
                     /note="attL1
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2670000
                     ApEinfo_fwdcolor=#00ff40
                     dnas_title=attL1
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_feature    2544..2547
                     /label=DEA-F
     CDS             2576..3229
                     /codon_start=1
                     /label=60_LoA3GX1
                     /translation="MWGAEGKSVRGGEERWAVPEGAPCLWASPAFDRSCLLDENTFTEN
                     FMNQMSPRKTYLCYKVEILDGDARIPLDEKKGFVRNKGANEPGGPRHAESYFLDRIRSW
                     NLDRDLHYRLTCFISWTPCDTCAQELADFLGKNSHVSLHIFASRIYSRFGYEDGLRTLQ
                     AAGAQIAIMASNEFEHCWENFVDHQGKPFQPWDGLEVVSQRLCNNLQAILQTQEN"
     CDS             3230..3277
                     /codon_start=1
                     /label=XTEN linker
                     /note="XTEN linker"
                     /translation="SGSETPGTSESATPES"
     CDS             3281..7549
                     /codon_start=1
                     /label=zCas9 fusion protein
                     /label=zCas9
                     /note="/dnas_title=zCas9
                     /vntifkey=4"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD
                     PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     sig_peptide     3284..3349
                     /label=3xFLAG
     misc_feature    3356..3376
                     /label=SV40 NLS
     CDS             3401..7501
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    3425..3427
                     /label=D10A
     misc_feature    7502..7549
                     /label=nucleoplasmin NLS
     misc_feature    7559..7852
                     /label=UGI-NLS
                     /note="UGI-NLS"
     misc_feature    7872..8149
                     /label=NOS term
                     /note="NOS term
                     vntifkey=21
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#f5010a
                     dnas_title=NOS term
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_feature    complement(7916..7939)
                     /label=PCR_primer
                     /note="PCR_primer
                     Nos-term-R2"
     misc_recomb     8171..8294
                     /label=attR5
                     /note="attR5
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2703000
                     ApEinfo_fwdcolor=#00ff00
                     dnas_title=attR5
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     promoter        8423..8424
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgtg
     2581 gggagctgag ggcaaatctg tcagaggagg agaagagagg tgggcagttc ctgaaggagc
     2641 accatgtctt tgggcatctc cagcctttga cagatcctgc ctgctcgatg aaaacacatt
     2701 cactgagaat tttatgaacc aaatgtcccc acgcaaaaca tacctctgtt acaaggtgga
     2761 gatacttgac ggagatgcta gaataccact cgacgaaaag aagggcttcg tccggaataa
     2821 aggtgctaat gagcccggcg gaccgcgcca tgctgaaagt tactttctcg accgcatcag
     2881 atcttggaat ttggaccggg acctccatta ccgcctgacc tgttttattt catggacgcc
     2941 ttgtgatact tgtgcgcaag aactggctga ttttctcgga aaaaactccc atgtgtccct
     3001 gcatattttc gcatctagga tttactctcg ctttggatat gaggacggat tgagaacact
     3061 tcaggcagct ggcgctcaaa ttgccattat ggcttctaat gagtttgagc attgctggga
     3121 gaactttgtt gaccaccagg gaaagccctt tcagccctgg gacggcttgg aagtggtgtc
     3181 tcaacgcctt tgcaataacc ttcaggcaat attgcaaacg caggagaact caggttctga
     3241 gacccctggc acaagtgagt cagcaacacc cgagtccacc atggattaca aggaccacga
     3301 cggggattac aaggaccacg acattgatta caaggatgat gatgacaaga tggctccgaa
     3361 gaagaagagg aaggttggca tccacggggt gccagctgct gacaagaagt actcgatcgg
     3421 cctcgctatt gggactaact ctgttggctg ggccgtgatc accgacgagt acaaggtgcc
     3481 ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga agaatctcat
     3541 tggcgctctc ctgttcgaca gcggcgagac ggctgaggct acgcggctca agcgcaccgc
     3601 ccgcaggcgg tacacgcgca ggaagaatcg catctgctac ctgcaggaga ttttctccaa
     3661 cgagatggcg aaggttgacg attctttctt ccacaggctg gaggagtcat tcctcgtgga
     3721 ggaggataag aagcacgagc ggcatccaat cttcggcaac attgtcgacg aggttgccta
     3781 ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact ccacagataa
     3841 ggcggacctc cgcctgatct acctcgctct ggcccacatg attaagttca ggggccattt
     3901 cctgatcgag ggggatctca acccggacaa tagcgatgtt gacaagctgt tcatccagct
     3961 cgtgcagacg tacaaccagc tcttcgagga gaaccccatt aatgcgtcag gcgtcgacgc
     4021 gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc tgatcgccca
     4081 gctgccgggc gagaagaaga acggcctgtt cgggaatctc attgcgctca gcctggggct
     4141 cacgcccaac ttcaagtcga atttcgatct cgctgaggac gccaagctgc agctctccaa
     4201 ggacacatac gacgatgacc tggataacct cctggcccag atcggcgatc agtacgcgga
     4261 cctgttcctc gctgccaaga atctgtcgga cgccatcctc ctgtctgata ttctcagggt
     4321 gaacaccgag attacgaagg ctccgctctc agcctccatg atcaagcgct acgacgagca
     4381 ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg agaagtacaa
     4441 ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg tacattgacg gcggggcctc
     4501 tcaggaggag ttctacaagt tcatcaagcc gattctggag aagatggacg gcacggagga
     4561 gctgctggtg aagctcaatc gcgaggacct cctgaggaag cagcggacat tcgataacgg
     4621 cagcatccca caccagattc atctcgggga gctgcacgct atcctgagga ggcaggagga
     4681 cttctaccct ttcctcaagg ataaccgcga gaagatcgag aagattctga ctttcaggat
     4741 cccgtactac gtcggcccac tcgctagggg caactcccgc ttcgcttgga tgacccgcaa
     4801 gtcagaggag acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg gcgctagcgc
     4861 tcagtcgttc atcgagagga tgacgaattt cgacaagaac ctgccaaatg agaaggtgct
     4921 ccctaagcac tcgctcctgt acgagtactt cacagtctac aacgagctga ctaaggtgaa
     4981 gtatgtgacc gagggcatga ggaagccggc tttcctgtct ggggagcaga agaaggccat
     5041 cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca aggaggacta
     5101 cttcaagaag attgagtgct tcgattcggt cgagatctct ggcgttgagg accgcttcaa
     5161 cgcctccctg gggacctacc acgatctcct gaagatcatt aaggataagg acttcctgga
     5221 caacgaggag aatgaggata tcctcgagga cattgtgctg acactcactc tgttcgagga
     5281 ccgggagatg atcgaggagc gcctgaagac ttacgcccat ctcttcgatg acaaggtcat
     5341 gaagcagctc aagaggagga ggtacaccgg ctgggggagg ctgagcagga agctcatcaa
     5401 cggcattcgg gacaagcagt ccgggaagac gatcctcgac ttcctgaaga gcgatggctt
     5461 cgcgaaccgc aatttcatgc agctgattca cgatgacagc ctcacattca aggaggatat
     5521 ccagaaggct caggtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc
     5581 tggctcgcca gctatcaaga aggggattct gcagaccgtg aaggttgtgg acgagctggt
     5641 gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt gagatggccc gggagaatca
     5701 gaccacgcag aagggccaga agaactcacg cgagaggatg aagaggatcg aggagggcat
     5761 taaggagctg gggtcccaga tcctcaagga gcacccggtg gagaacacgc agctgcagaa
     5821 tgagaagctc tacctgtact acctccagaa tggccgcgat atgtatgtgg accaggagct
     5881 ggatattaac aggctcagcg attacgacgt cgatcatatc gttccacagt cattcctgaa
     5941 ggatgactcc attgacaaca aggtcctcac caggtcggac aagaaccggg gcaagtctga
     6001 taatgttcct tcagaggagg tcgttaagaa gatgaagaac tactggcgcc agctcctgaa
     6061 tgccaagctg atcacgcagc ggaagttcga taacctcaca aaggctgaga ggggcgggct
     6121 ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac ggcagatcac
     6181 taagcacgtt gcgcagattc tcgactcacg gatgaacact aagtacgatg agaatgacaa
     6241 gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg acttcaggaa
     6301 ggatttccag ttctacaagg ttcgggagat caacaattac caccatgccc atgacgcgta
     6361 cctgaacgcg gtggtcggca cagctctgat caagaagtac ccaaagctcg agagcgagtt
     6421 cgtgtacggg gactacaagg tttacgatgt gaggaagatg atcgccaagt cggagcagga
     6481 gattggcaag gctaccgcca agtacttctt ctactctaac attatgaatt tcttcaagac
     6541 agagatcact ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga cgaacggcga
     6601 gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtcagga aggttctctc
     6661 catgccacaa gtgaatatcg tcaagaagac agaggtccag actggcgggt tctctaagga
     6721 gtcaattctg cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg actgggatcc
     6781 gaagaagtac ggcgggttcg acagccccac tgtggcctac tcggtcctgg ttgtggcgaa
     6841 ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg ggatcacgat
     6901 tatggagcgc tccagcttcg agaagaaccc gatcgatttc ctggaggcga agggctacaa
     6961 ggaggtgaag aaggacctga tcattaagct ccccaagtac tcactcttcg agctggagaa
     7021 cggcaggaag cggatgctgg cttccgctgg cgagctgcag aaggggaacg agctggctct
     7081 gccgtccaag tatgtgaact tcctctacct ggcctcccac tacgagaagc tcaagggcag
     7141 ccccgaggac aacgagcaga agcagctgtt cgtcgagcag cacaagcatt acctcgacga
     7201 gatcattgag cagatttccg agttctccaa gcgcgtgatc ctggccgacg cgaatctgga
     7261 taaggtcctc tccgcgtaca acaagcaccg cgacaagcca atcagggagc aggctgagaa
     7321 tatcattcat ctcttcaccc tgacgaacct cggcgcccct gctgctttca agtacttcga
     7381 cacaactatc gatcgcaaga ggtacacaag cactaaggag gtcctggacg cgaccctcat
     7441 ccaccagtcg attaccggcc tctacgagac gcgcatcgac ctgtctcagc tcgggggcga
     7501 caagcggcca gcggcgacga agaaggcggg gcaggcgaag aagaagaagg gagacggatc
     7561 aggcgggtca acaaacctca gtgacatcat agagaaggaa actggtaagc aactggttat
     7621 tcaagagagc attctgatgc tccctgagga agtcgaggaa gttataggaa acaagcctga
     7681 gagcgatata ctcgtgcata cggcgtatga cgagagcacg gatgaaaatg tgatgctctt
     7741 gaccagcgat gcgccagaat ataaaccctg ggcactggtt attcaggact ctaacgggga
     7801 aaataaaatc aagatgctct caggtggctc cccaaagaag aaacgcaagg tttagtggcg
     7861 gccgccgacg tccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     7921 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     7981 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     8041 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     8101 cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc ttccggaaag
     8161 ggcgaattcg caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc
     8221 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     8281 atccagtcac tatgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     8341 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     8401 aaaaatatat catcatgcct cctc
//
