LOCUS       sample_580              7146 bp    DNA     circular SYN 02-AUG-2024
DEFINITION  sample_580.
ACCESSION   sample_580
VERSION     sample_580
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(1959..2547)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             complement(5028..5285)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(1119..1376)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(4126..4359)
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    complement(5829..5963)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    complement(4856..4981)
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(3519..3634)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        6011..6115
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6116..6976
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2902..3173)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="97.8"
                     /match_length="97.8"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(3180..3458)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="92.1"
                     /fragment="True"
                     /other="enhancer"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1424..1942)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="98.3"
                     /match_length="84.0"
                     /fragment="True"
                     /other="CDS"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     intron          complement(2734..2830)
                     /note="pLannotate"
                     /label="SV40 intron"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(5333..5662)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="53.4"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4534..4872)
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="22.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2573..2641)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="85.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3649..3749)
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2642..2661
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.5"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(2872..2888)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="15.7"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt ggatcccgcg cgcgggcccg
     2581 cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga tctgagtccg gtagcgctag
     2641 cgcggccgct ctagaactag tctcgaggaa ttcggatcgg ccgcgggtac aattccgcag
     2701 cttttagagc agaagtaaca cttccgtaca ggcctagaag taaaggcaac atccactgag
     2761 gagcagttct ttgatttgca ccaccaccgg atcggggacc tgaaataaaa gacaaaaaga
     2821 ctaaacttac cagttaactt tctggttttt cagttcctcg atcgactcta gggccgccac
     2881 cgcggtggct gatcccgctc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg
     2941 ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc
     3001 acctctccgc ctcgccccgc cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc
     3061 cccccccccc cccccccccg cccccatcgc tgcacaaaat aattaaaaaa taaataaata
     3121 caaaattggg ggtggggagg ggggggagat ggggagagtg aagcagaacg tggcctcgag
     3181 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     3241 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     3301 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     3361 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg tcgttgggcg
     3421 gtcagccagg cgggccattt accgtaagtt atgtaacgac cagaggtacc ttaattaatc
     3481 gataaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
     3541 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     3601 cactgtaccc cccaatcccc ccttttcttt taaaggcgat accgtcgaga tccgttcact
     3661 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     3721 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     3781 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     3841 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     3901 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     3961 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     4021 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     4081 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     4141 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     4201 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     4261 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     4321 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     4381 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     4441 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     4501 aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     4561 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     4621 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     4681 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     4741 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     4801 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     4861 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
     4921 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
     4981 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     5041 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     5101 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     5161 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
     5221 aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac
     5281 gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct
     5341 tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc
     5401 tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca
     5461 agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct
     5521 tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct
     5581 aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcttt
     5641 gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca aatacaattt
     5701 ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa ccacaggtaa
     5761 ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt agcttgagtt
     5821 atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5881 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5941 ttataagctg caataaacaa gttgatctcc cgatccgtcg acgtcaggtg gcacttttcg
     6001 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6061 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6181 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6241 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6301 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6361 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6421 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6481 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6541 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6601 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6661 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6721 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6781 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6841 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6901 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6961 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7141 atcttc
//
