LOCUS       pETcon-Aga2p-A𝜷42       6262 bp ds-DNA     circular     29-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_binding    1..20
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     26..46
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        63..81
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     64..83
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        311..763
                     /label="GAL1 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    803..1063
                     /label="Aga2p"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1094..1120
                     /label="HA Tag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1133..1177
                     /label="GS linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1133..1177
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GGGGSGGGGSGGGGS"
     misc_feature    1184..1312
                     /label="AB42"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1313..1324
                     /label="GS linker"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1313..1324
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GGGS"
     misc_feature    1325..1354
                     /label="c-myc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(1683..1710)
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      1883..2189
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(2378..3200)
                     /label="TRP1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(3641..4151)
                     /label="CEN-ARS pRS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4481..5140
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      5238..5920
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        6226..6256
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_binding    6260..6262
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
       61 gaaattaacc ctcactaaag ggaacaaaag ctggtaccaa ttccttgaat tttcaaaaat
      121 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc
      181 accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat
      241 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt
      301 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
      361 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
      421 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
      481 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
      541 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
      601 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac
      661 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
      721 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat cgaattccct
      781 acttcataca ttttcaatta agatgcagtt acttcgctgt ttttcaatat tttctgttat
      841 tgcttcagtt ttagcacagg aactgacaac tatatgcgag caaatcccct caccaacttt
      901 agaatcgacg ccgtactctt tgtcaacgac tactattttg gccaacggga aggcaatgca
      961 aggagttttt gaatattaca aatcagtaac gtttgtcagt aattgcggtt ctcacccctc
     1021 aacaactagc aaaggcagcc ccataaacac acagtatgtt tttaaggaca atagctcgac
     1081 gattgaaggt agatacccat acgacgttcc agactacgct ctgcaggcta gtggtggagg
     1141 aggctctggt ggaggcggta gcggaggcgg agggtcgact agtATGGACG CGGAGTTTCG
     1201 TCACGATAGT GGATATGAGG TTCATCATCA AAAGCTAGTC TTCTTTGCGG AGGATGTAGG
     1261 TTCTAATAAA GGTGCCATAA TCGGACTAAT GGTCGGCGGA GTTGTGATAG CTgggggcgg
     1321 atccgaacaa aagcttattt ctgaagagga cttgtaatag agatctgata acaacagtgt
     1381 agatgtaaca aaatcgactt tgttcccact gtacttttag ctcgtacaaa atacaatata
     1441 cttttcattt ctccgtaaac aacatgtttt cccatgtaat atccttttct atttttcgtt
     1501 ccgttaccaa ctttacacat actttatata gctattcact tctatacact aaaaaactaa
     1561 gacaatttta attttgctgc ctgccatatt tcaatttgtt ataaattcct ataatttatc
     1621 ctattagtag ctaaaaaaag atgaatgtga atcgaatcct aagagaattg agctccaatt
     1681 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     1741 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1801 gtaatagcga agaggcccgc accgatcgcc tttcccaaca gttgcgcagc ctgaatggcg
     1861 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1921 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     2101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     2161 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     2221 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2281 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     2341 cttacgcatc tgtgcggtat ttcacaccgc atagatcggc aagtgcacaa acaatactta
     2401 aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg
     2461 ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca
     2521 tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt
     2581 aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt
     2641 tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa
     2701 gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa
     2761 ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg
     2821 ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc
     2881 aagtatttcg gagtgcctga actattttta tatgctttta caagacttga aattttcctt
     2941 gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca
     3001 gcatcggaat ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc
     3061 aatggaccag aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc
     3121 ttaatcacgt atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct
     3181 tttctttttt cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga
     3241 ttgtacgtaa ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg
     3301 cgtcataact gcaaagtaca catatattac gatgctgttc tattaaatgc ttcctatatt
     3361 atatatatag taatgtcgtg atctatggtg cactctcagt acaatctgct ctgatgccgc
     3421 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3481 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3541 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3601 ataggttaat gtcatgataa taatggtttc ttagacggat cgcttgcctg taacttacac
     3661 gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt tatttatttt
     3721 tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt acggaatgaa
     3781 gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt acatatatat
     3841 ttattagaca agaaaagcag attaaataga tatacattcg attaacgata agtaaaatgt
     3901 aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca attcggcatt
     3961 aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat ccccctagag
     4021 tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt ttactttcta
     4081 tttttaattt atatatttat attaaaaaat ttaaattata attattttta tagcacgtga
     4141 tgaaaaggac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4201 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4261 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4321 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4381 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4441 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4501 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4561 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4621 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4681 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4741 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4801 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4861 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4921 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4981 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5041 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5101 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5161 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5221 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5281 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5341 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5401 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5461 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5521 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5581 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5641 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5701 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5761 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5821 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5881 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5941 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6001 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6061 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6121 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6181 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6241 tccggctcgt atgttgtgtg ga
//