LOCUS       Exported                8276 bp ds-DNA     circular SYN 30-JUL-2024
DEFINITION  Localization of LSSmGFP fluorescent protein to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Campbell, BC, Nabel, EM, Murdock, MH, Lao-Peregrin, C, Tsoulfas, P, 
            Blackmore, MG, Lee, FS, Liston, C, Morishita, H, Petsko, GA
  TITLE     mGreenLantern: a bright monomeric fluorescent protein with rapid 
            expression and cell filling properties for neuronal imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30710-30721. doi: 
            10.1073/pnas.2000942117. Epub 2020 Nov 18.
  PUBMED    33208539
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 2, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..1050,3577..8276)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=Geneious name: source synthetic DNA
                     construct
                     /label=Geneious name: source synthetic DNA construct
                     /note="Geneious name: source synthetic DNA construct"
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=Geneious name: source synthetic DNA 
                     construct"
     source          2791..2820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct"
     enhancer        1..380
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="Geneious name: CMV enhancer"
     promoter        381..584
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="Geneious name: CMV promoter"
     primer_bind     535..555
                     /locus_tag="CMV-F"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="Geneious name: CMV-F"
     misc_feature    628..660
                     /locus_tag="4mt_HindIII_vec_pR"
                     /label=4mt_HindIII_vec_pR
                     /note="Geneious type: primer_bind_reverse"
                     /note="Geneious name: 4mt HindIII vec pR"
     primer_bind     629..648
                     /locus_tag="T7"
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="Geneious name: T7"
     promoter        629..647
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="Geneious name: T7 promoter"
     misc_feature    655..1050
                     /locus_tag="codon-optimized sequence for synthesis"
                     /label=codon-optimized sequence for synthesis
                     /note="Geneious name: codon-optimized sequence for
                     synthesis"
     primer_bind     655..676
                     /locus_tag="4mt-opti_pF"
                     /label=4mt-opti_pF
                     /note="Geneious name: 4mt-opti pF"
     misc_feature    661..1050
                     /locus_tag="ATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGC
                     TCGGCCCG GCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCCATG"
                     /label=ATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGC
                     TCGG...
                     /label=ATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGC
                     TCGGCCCG GCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCCATG
                     /note="Geneious type: Editing History Replacement"
                     /note="Geneious name: 
                     ATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCG 
                     GCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCCATGTCCGTCCTG 
                     ACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCG 
                     CCAAGATCCATTCGTTGGGGAAGCTTATGTCCGTCCTGACGCCGCTGCTGCTGCGGGG 
                     CTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGG 
                     GATCCCATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGC 
                     GGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGAAGCTTGCCACCATGGTGAG 
                     CAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGAC 
                     GTAAACGGCCACAAGTTCAGCGTCCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCA 
                     AGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCT 
                     CGTGACCACCTTAGGCTACGGCGTGGCCTGCTTCGCCCGCTACCCCGACCACATGAAG 
                     CAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT 
                     CTTTCAAGGACGACGGTACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACAC 
                     CCTGGTGAACCGCATCGTGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTG 
                     GGGCACAAGCTGGAGTACAACTTCAACAGCCACAAGGTCTATATCACGGCCGACAAGC 
                     AGAAGAACGGCATCAAGGCTAACTTCAAGACCCGCCACAACGTTGAGGACGGCGGCGT 
                     GCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG 
                     CCCGACAACCACTACCTGAGCCATCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGC 
                     GCGATCACATGGTCCTGAAGGAGAGGGTGACCGCCGCCGGGATTACACATGACATGG"
     CDS             667..741
                     /locus_tag="COX8 presequence"
                     /label=COX8 presequence
                     /note="Geneious name: COX8 presequence"
     CDS             763..837
                     /locus_tag="COX8 presequence(1)"
                     /label=COX8 presequence
                     /label=COX8 presequence(1)
                     /note="Geneious name: COX8 presequence"
     CDS             859..933
                     /locus_tag="COX8 presequence(2)"
                     /label=COX8 presequence
                     /label=COX8 presequence(2)
                     /note="Geneious name: COX8 presequence"
     CDS             955..1029
                     /locus_tag="COX8 presequence(3)"
                     /label=COX8 presequence
                     /label=COX8 presequence(3)
                     /note="Geneious name: COX8 presequence"
     misc_feature    1024..1050
                     /locus_tag="4mt-opti_pR"
                     /label=4mt-opti_pR
                     /note="Geneious type: primer_bind_reverse"
                     /note="Geneious name: 4mt-opti pR"
     misc_feature    1051..1080
                     /locus_tag="linker"
                     /label=linker
     misc_feature    join(1081..1509,1513..1929,2674..2790)
                     /locus_tag="PcSBP"
                     /label=PcSBP
     misc_feature    1081..2790
                     /locus_tag="iPASnFR-L"
                     /label=iPASnFR-L
     misc_feature    1510..1512
                     /locus_tag="PcSBP(1)"
                     /label=PcSBP
                     /label=PcSBP(1)
     misc_feature    1936..2667
                     /locus_tag="iGluSnFR3"
                     /label=iGluSnFR3
     misc_feature    2821..2826
                     /locus_tag="EcoR1"
                     /label=EcoR1
     CDS             2827..3510
                     /locus_tag="Geneious name: mScarlet3"
                     /label=Geneious name: mScarlet3
                     /note="Geneious name: mScarlet3"
     misc_feature    3511..3576
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     CDS             3577..3579
                     /locus_tag="LSSmGFP"
                     /label=LSSmGFP
                     /note="Geneious name: LSSmGFP"
     promoter        3595..3613
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="Geneious name: SP6 promoter"
     primer_bind     3596..3613
                     /locus_tag="SP6"
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="Geneious name: SP6"
     primer_bind     3633..3650
                     /locus_tag="BGH-rev"
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
                     /note="Geneious name: BGH-rev"
     polyA_signal    3639..3863
                     /locus_tag="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="Geneious name: bGH poly(A) signal"
     rep_origin      3909..4337
                     /direction=RIGHT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="Geneious name: f1 ori"
     primer_bind     3996..4015
                     /locus_tag="F1ori-R"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="Geneious name: F1ori-R"
     primer_bind     4206..4227
                     /locus_tag="F1ori-F"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="Geneious name: F1ori-F"
     primer_bind     4346..4366
                     /locus_tag="pBABE 3'"
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="Geneious name: pBABE 3'"
     promoter        4351..4680
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="Geneious name: SV40 promoter"
     rep_origin      4531..4666
                     /direction=BOTH
                     /locus_tag="SV40 ori"
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="Geneious name: SV40 ori"
     primer_bind     4593..4612
                     /locus_tag="SV40pro-F"
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="Geneious name: SV40pro-F"
     CDS             4747..5541
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /locus_tag="NeoR/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="Geneious name: aph(3')-II (or nptII) CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4801..4820
                     /locus_tag="Neo-R"
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="Geneious name: Neo-R"
     primer_bind     5411..5430
                     /locus_tag="Neo-F"
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="Geneious name: Neo-F"
     polyA_signal    5715..5836
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Geneious name: SV40 poly(A) signal"
     primer_bind     5752..5771
                     /locus_tag="SV40pA-R"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="Geneious name: SV40pA-R"
     primer_bind     5806..5825
                     /locus_tag="EBV-rev"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="Geneious name: EBV-rev"
     primer_bind     5885..5901
                     /locus_tag="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="Geneious name: M13 rev"
     primer_bind     5885..5901
                     /locus_tag="M13 Reverse"
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="Geneious name: M13 Reverse"
     primer_bind     5898..5920
                     /locus_tag="M13/pUC Reverse"
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="Geneious name: M13/pUC Reverse"
     protein_bind    5909..5925
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Geneious name: lac operator"
     promoter        5933..5963
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="Geneious name: lac promoter"
     protein_bind    5978..5999
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="Geneious name: CAP binding site"
     primer_bind     6116..6133
                     /locus_tag="L4440"
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="Geneious name: L4440"
     rep_origin      complement(6287..6875)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="Geneious name: ori"
     primer_bind     6367..6386
                     /locus_tag="pBR322ori-F"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="Geneious name: pBR322ori-F"
     CDS             7046..7906
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Geneious name: bla CDS"
                     /translation="MPMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCND
                     TARPTLTGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCH
                     CYRHRGVTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGY
                     GSTA*FSYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAHH
                     WKTFFGAKTLKDLTAVEIQFDVTHSCTQLIFSIFYFHQRFWVSKNRKAKCRKKGNKGDT
                     EMLNTH"
     primer_bind     7669..7688
                     /locus_tag="Amp-R"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="Geneious name: Amp-R"
     promoter        7907..8011
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious name: bla promoter"
     primer_bind     8086..8105
                     /locus_tag="pRS-marker"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /note="Geneious name: pRS-marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgt cagtgctgac cccactcctg ctcagagggt tgactggttc cgcacgacgc
      721 cttccagttc cgagggccaa gatacatagt cttggagatc ctatgagcgt tctgacgcca
      781 ttgttgctgc gcggactgac ggggagcgca aggcggctcc ctgtcccgag ggcaaagatc
      841 catagcctgg gcaaactgat gtccgtcttg acccctcttc tcctgagagg acttactggt
      901 tctgctcgca ggttgccggt tccaagagca aaaatacata gcctcgggga ccctatgtcc
      961 gtacttacac ctctccttct tagaggactg acaggcagcg ccaggaggct gcccgtccca
     1021 cgggctaaaa ttcactcttt gggaaagctt ggtggtggcg gatcagaagg aggcggtagc
     1081 aacacgttga cggtttacaa ttggggtgat tacatcgacc ctgcattaat ccgtaagttc
     1141 gagaagcaaa cgggtataaa ggttatatat cagacgtttg actccaatga agcaatgatg
     1201 gcaaagattg ctcaaggtgg agcaacgttc gacgtagcag ttccatctga gtacgcgatc
     1261 tctaagatga aggaagatgg attgctgatc cccttagacc attcaaaatt acctaacctc
     1321 aaatacatta atccgcgctt cttaaattta agcttcgacc ccggaaacag atactctgtc
     1381 ccctacttct ggggtaccgt ggggatagtt tacaatcccg gcatgttggg tggtaagaag
     1441 atcacttctt ggaacgactt gtgggatcca gacctgcgca atcagatcct gcttgttgac
     1501 ggagctcgtg cggtaatcgg aatgggactt aactcactcc actattcact caacgacacc
     1561 aataaagagc atctcaagaa ggccaaagag aagttggaca gactcgtgcc aaatgtgaaa
     1621 gccatagtcg gggatgaaat caagatgttg ctggcgaatg aggaagcagc gataggagtt
     1681 gtgtggagtg gggacgctgc ggagatcatc gccgaaaatg acaagttgga ttacgtggtg
     1741 cccaaagagg gatcgaactt gtggttcgac aatatggtga ttccaaagac cgcgaagaat
     1801 attgaaggag cacataaatt cattaacttc atgctggacc caaagaatgc ggcgcagaac
     1861 gccgagtttg ttggctattc aaccccaaat aagaaggctt tggattacat gcctaaggaa
     1921 atcacagagt ctaagaggca cacggtctat atcaccgccg acaagcagaa gaacggcatc
     1981 aaggcgaact tcaagatcca ccttaacgtg gaggacggca gcgtgcagct cgccgaccac
     2041 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2101 agctatcagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2161 gagttcgtga ccgccaccgg gaactctctc ggcatggacg agctgtacaa gggcggtacc
     2221 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     2341 accaacggca agctaaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2401 cccaccctcg tgaccaccct gagctacggc gtgcagtgct tcgcccgcta ccccgaccac
     2461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2521 atcagcttca aggacgacgg aacctacaag acccgcgccg aggtgaagtt agagggcgac
     2581 accctggtga accgcatcga gctaaagggc atcgacttca aggaggacgg caacatcctg
     2641 gggcacaagc tggagtacaa ctttaactat catgacaagc ggttctatcc cgatttcgac
     2701 tcaatcggca atttagaagt ttacaagaat cttggaaagc gtatgttggc ttactacaat
     2761 gaactgttcc tgcaatttaa gatgcaccgc aatgctgtgg gccaggacac gcaggagcgt
     2821 gaattcgata gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
     2881 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2941 ggcacccaga ccgccaagct gagggtgacc aagggtggcc ccctgccctt ctcctgggac
     3001 atcctgtccc ctcagttcat gtacggctcc agggccttca cgaagcaccc cgccgacatc
     3061 cccgactact ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3121 gaggacggcg gcgccgtgtc cgtggcccag gacacctccc tggaggacgg caccctgatc
     3181 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
     3241 acaatgggct gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc
     3301 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
     3361 acctacaggg ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga ccgcaagttg
     3421 gacatcacat cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgtggcc
     3481 cgccactcca ccggcggctc cggtggctcc gactacaaag accatgacgg tgattataaa
     3541 gatcatgaca tcgattacaa ggatgacgat gacaagtaat ctagagggcc ctattctata
     3601 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     3661 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3721 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3781 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3841 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3901 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3961 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4021 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4081 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4141 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4201 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4261 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4321 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4501 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4681 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     4741 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4801 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4861 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4921 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4981 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5041 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5101 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5161 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5221 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5281 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5341 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5401 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5461 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     5521 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     5581 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5641 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5701 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5761 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5821 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     5881 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5941 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6001 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6061 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7561 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7921 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7981 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8041 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8101 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     8161 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     8221 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
