LOCUS       Exported                7998 bp ds-DNA     circular SYN 30-JUL-2024
DEFINITION  cyto-Grx1Q-roGFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7998)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 2, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..1036,3533..7998)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=Geneious name: source synthetic DNA
                     construct
                     /label=Geneious name: source synthetic DNA construct
                     /note="Geneious name: source synthetic DNA construct"
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=Geneious name: source synthetic DNA 
                     construct"
     source          2747..2776
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct"
     misc_feature    34..35
                     /locus_tag="fixed from Ben's"
                     /label=fixed from Ben's
     primer_bind     44..63
                     /locus_tag="pRS-marker"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /note="Geneious name: pRS-marker"
     enhancer        235..614
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="Geneious name: CMV enhancer"
     misc_feature    440
                     /locus_tag="fixed from Ben's(1)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(1)
     promoter        615..818
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="Geneious name: CMV promoter"
     primer_bind     769..789
                     /locus_tag="CMV-F"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="Geneious name: CMV-F"
     primer_bind     863..882
                     /locus_tag="T7"
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="Geneious name: T7"
     promoter        863..881
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="Geneious name: T7 promoter"
     misc_feature    927..943
                     /locus_tag="Geneious type: primer_bind_reverse"
                     /label=Geneious type: primer_bind_reverse
                     /note="Geneious type: primer_bind_reverse"
                     /note="Geneious name: pcDNA3-Grx1Q_vec_pR"
     misc_feature    938..942
                     /locus_tag="fixed from Ben's(2)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(2)
     misc_feature    join(944..1036,3533..3535)
                     /locus_tag="Geneious type: ORF"
                     /label=Geneious type: ORF
                     /note="Geneious type: ORF"
     misc_feature    944..1036
                     /locus_tag="Geneious type: motif"
                     /label=Geneious type: motif
                     /note="Geneious type: motif"
                     /note="Geneious name: 3xNLS"
     misc_feature    944..946
                     /locus_tag="Start"
                     /label=Start
     CDS             956..976
                     /codon_start=1
                     /locus_tag="SV40 NLS(2)"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /label=SV40 NLS(2)
                     /note="Geneious name: nuclear localization signal of SV40 
                     (simian virus 40) large T antigen CDS"
                     /translation="PKKKRKV"
     CDS             980..1000
                     /codon_start=1
                     /locus_tag="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="Geneious name: nuclear localization signal of SV40 
                     (simian virus 40) large T antigen CDS"
                     /translation="PKKKRKV"
     CDS             1004..1024
                     /codon_start=1
                     /locus_tag="SV40 NLS(1)"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /label=SV40 NLS(1)
                     /note="Geneious name: nuclear localization signal of SV40 
                     (simian virus 40) large T antigen CDS"
                     /translation="PKKKRKV"
     misc_feature    1037..2746
                     /locus_tag="iPASnFR-H"
                     /label=iPASnFR-H
     misc_feature    2777..2782
                     /locus_tag="EcoR1"
                     /label=EcoR1
     CDS             2783..3466
                     /locus_tag="Geneious name: mScarlet3"
                     /label=Geneious name: mScarlet3
                     /note="Geneious name: mScarlet3"
     misc_feature    3467..3532
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     misc_feature    3533..3535
                     /locus_tag="Stop"
                     /label=Stop
     gene            3533..3535
                     /locus_tag="Geneious name: roGFP2"
                     /label=Geneious name: roGFP2
                     /note="Geneious name: roGFP2"
     promoter        3551..3569
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="Geneious name: SP6 promoter"
     primer_bind     3552..3569
                     /locus_tag="SP6"
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="Geneious name: SP6"
     primer_bind     3589..3606
                     /locus_tag="BGH-rev"
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
                     /note="Geneious name: BGH-rev"
     polyA_signal    3595..3819
                     /locus_tag="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="Geneious name: bGH poly(A) signal"
     rep_origin      3865..4293
                     /direction=RIGHT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="Geneious name: f1 ori"
     primer_bind     3952..3971
                     /locus_tag="F1ori-R"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="Geneious name: F1ori-R"
     misc_feature    4022..4023
                     /locus_tag="fixed from Ben's(3)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(3)
     primer_bind     4162..4183
                     /locus_tag="F1ori-F"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="Geneious name: F1ori-F"
     misc_feature    4236..4237
                     /locus_tag="fixed from Ben's(5)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(5)
     primer_bind     4302..4322
                     /locus_tag="pBABE 3'"
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="Geneious name: pBABE 3'"
     promoter        4307..4636
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="Geneious name: SV40 promoter"
     rep_origin      4487..4622
                     /direction=BOTH
                     /locus_tag="SV40 ori"
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="Geneious name: SV40 ori"
     primer_bind     4549..4568
                     /locus_tag="SV40pro-F"
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="Geneious name: SV40pro-F"
     CDS             4703..5497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /locus_tag="NeoR/KanR"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="Geneious name: aph(3')-II (or nptII) CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4757..4776
                     /locus_tag="Neo-R"
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="Geneious name: Neo-R"
     primer_bind     5367..5386
                     /locus_tag="Neo-F"
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="Geneious name: Neo-F"
     polyA_signal    5671..5792
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Geneious name: SV40 poly(A) signal"
     primer_bind     5708..5727
                     /locus_tag="SV40pA-R"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="Geneious name: SV40pA-R"
     primer_bind     5762..5781
                     /locus_tag="EBV-rev"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="Geneious name: EBV-rev"
     primer_bind     5841..5857
                     /locus_tag="M13 Reverse"
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="Geneious name: M13 Reverse"
     primer_bind     5841..5857
                     /locus_tag="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="Geneious name: M13 rev"
     primer_bind     5854..5876
                     /locus_tag="M13/pUC Reverse"
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="Geneious name: M13/pUC Reverse"
     protein_bind    5865..5881
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Geneious name: lac operator"
     promoter        5889..5919
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="Geneious name: lac promoter"
     protein_bind    5934..5955
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="Geneious name: CAP binding site"
     primer_bind     6072..6089
                     /locus_tag="L4440"
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="Geneious name: L4440"
     rep_origin      complement(6243..6831)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="Geneious name: ori"
     primer_bind     6323..6342
                     /locus_tag="pBR322ori-F"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="Geneious name: pBR322ori-F"
     misc_feature    6438..6439
                     /locus_tag="fixed from Ben's(4)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(4)
     misc_feature    6641
                     /locus_tag="fixed from Ben's(6)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(6)
     misc_feature    6684
                     /locus_tag="fixed from Ben's(7)"
                     /label=fixed from Ben's
                     /label=fixed from Ben's(7)
     CDS             7002..7862
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Geneious name: bla CDS"
                     /translation="MPMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCND
                     TARPTLTGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCH
                     CYRHRGVTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGY
                     GSTA*FSYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAHH
                     WKTFFGAKTLKDLTAVEIQFDVTHSCTQLIFSIFYFHQRFWVSKNRKAKCRKKGNKGDT
                     EMLNTH"
     primer_bind     7625..7644
                     /locus_tag="Amp-R"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="Geneious name: Amp-R"
     promoter        7863..7967
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious name: bla promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggcc accatgggat cagatccaaa
      961 aaagaagaga aaggtagacc cgaagaaaaa gcgtaaggtc gatcccaaaa agaaacggaa
     1021 agtgggatca ccggtcaaca cgttgacggt ttacaattgg ggtgattaca tcgaccctgc
     1081 attaatccgt aagttcgaga agcaaacggg tataaaggtt atatatcaga cgtttgactc
     1141 caatgaagca atgatggcaa agattgctca aggtggagca acgttcgacg tagcagttcc
     1201 atctgagtac gcgatctcta agatgaagga agatggattg ctgatcccct tagaccattc
     1261 aaaattacct aacctcaaat acattaatcc gcgcttctta aatttaagct tcgaccccgg
     1321 aaacagatac tctgtcccct acttctgggg taccgtgggg atagtttaca atcccggcat
     1381 gttgggtggt aagaagatca cttcttggaa cgacttgtgg gatccagacc tgcgcaatca
     1441 gatcctgctt gttgacggag ctcgtgaggt aatcggaatg ggacttaact cactccacta
     1501 ttcactcaac gacaccaata aagagcatct caagaaggcc aaagagaagt tggacagact
     1561 cgtgccaaat gtgaaagcca tagtcgggga tgaaatcaag atgttgctgg cgaatgagga
     1621 agcagcgata ggagttgtgt ggagtgggga cgctgcggag atcatcgccg aaaatgacaa
     1681 gttggattac gtggtgccca aagagggatc gaacttgtgg ttcgacaata tggtgattcc
     1741 aaagaccgcg aagaatattg aaggagcaca taaattcatt aacttcatgc tggacccaaa
     1801 gaatgcggcg cagaacgccg agtacgttgg ctattcaacc ccaaataaga aggctttgga
     1861 ttacatgcct aaggaaatca cagagtctaa gaggcacacg gtctatatca ccgccgacaa
     1921 gcagaagaac ggcatcaagg cgaacttcaa gatccacctt aacgtggagg acggcagcgt
     1981 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2041 cgacaaccac tacctgagct atcagtccgt gctgagcaaa gaccccaacg agaagcgcga
     2101 tcacatggtc ctgctggagt tcgtgaccgc caccgggaac tctctcggca tggacgagct
     2161 gtacaagggc ggtaccggag ggagcatgag caagggcgag gagctgttca ccggggtggt
     2221 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga
     2281 gggcgagggc gatgccacca acggcaagct aaccctgaag ttcatctgca ccaccggcaa
     2341 gctgcccgtg ccctggccca ccctcgtgac caccctgagc tacggcgtgc agtgcttcgc
     2401 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2461 cgtccaggag cgcaccatca gcttcaagga cgacggaacc tacaagaccc gcgccgaggt
     2521 gaagttagag ggcgacaccc tggtgaaccg catcgagcta aagggcatcg acttcaagga
     2581 ggacggcaac atcctggggc acaagctgga gtacaacttt aactatcatg acaagcggtt
     2641 ctatcccgat ttcgactcaa tcggcaattt agaagtttac aagaatcttg gaaagcgtat
     2701 gttggcttac tacaatgaac tgttcctgca atttaagatg caccgcaatg ctgtgggcca
     2761 ggacacgcag gagcgtgaat tcgatagcac cgaggcagtg atcaaggagt tcatgcggtt
     2821 caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     2881 gggccgcccc tacgagggca cccagaccgc caagctgagg gtgaccaagg gtggccccct
     2941 gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcacgaa
     3001 gcaccccgcc gacatccccg actactggaa gcagtccttc cccgagggct tcaagtggga
     3061 gcgcgtgatg aacttcgagg acggcggcgc cgtgtccgtg gcccaggaca cctccctgga
     3121 ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc
     3181 cgtaatgcag aagaagacaa tgggctggga agcatccacc gagcggttgt accccgagga
     3241 cgtcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg gccgctacct
     3301 ggcggacttc aagaccacct acagggccaa gaagcccgtg cagatgcccg gcgccttcaa
     3361 catcgaccgc aagttggaca tcacatccca caacgaggac tacaccgtgg tggaacagta
     3421 cgaacgctcc gtggcccgcc actccaccgg cggctccggt ggctccgact acaaagacca
     3481 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agtaatctag
     3541 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     3601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3721 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3781 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     3841 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3961 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4021 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4081 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4141 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4201 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4261 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4561 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4621 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     4681 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4861 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5221 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5521 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5641 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     5701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     5821 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5881 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5941 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6001 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6061 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta aagagttctt gaagtggtgg
     6661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7981 aaagtgccac ctgacgtc
//
