LOCUS       pV238-vPE       10473 bp DNA     circular SYN 02-AUG-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        434..637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             747..767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             768..4868
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the H840A mutation in the HNH catalytic domain"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYAHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4926..4946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             4971..7001
                     /codon_start=1
                     /label=M-Mulv Reverse Transcriptase
                     /note="color: #99ccff"
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     CDS             7002..7097
                     /label=2XSGGS XTEN 2XSGGS
                     /note="color: #993366; direction: RIGHT"
     CDS             7098..7679
                     /label=La (SSB) 1-194
                     /note="color: #993366; direction: RIGHT"
     CDS             7692..7745
                     /label=Bipartite SV40 NLS
                     /note="color: #993366; direction: RIGHT"
     CDS             7746..7781
                     /label=GSG-cmycNLS
                     /note="color: #993366; direction: RIGHT"
     CDS             7790..7807
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     polyA_signal    7836..8060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8131..8147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8155..8171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(8179..8185,8186..8203,8204..8209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8179 .. 8185 / #ffffff / -10
                        2: 8186 .. 8203 / #ffffff
                        3: 8204 .. 8209 / #ffffff / -35"
     protein_bind    8224..8245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8533..9121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(9292..10083,10084..10152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9292 .. 10083 / #ccffcc
                        2: 10084 .. 10152 / #ccffcc / signal sequence
                     Cleavage site after base 10083"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10153..10257)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      661 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga
      721 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
      781 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      841 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      901 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      961 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1021 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1081 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1141 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1201 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1261 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1321 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1381 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1441 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1501 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1561 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1621 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1681 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1741 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1801 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1861 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1921 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     1981 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2041 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2101 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2161 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2221 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2281 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2341 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2401 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2461 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2521 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2581 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2641 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2701 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2761 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2821 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2881 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2941 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3001 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3061 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3121 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3181 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3241 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3301 ttctggccga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3361 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3421 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3481 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3541 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3601 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3661 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactacgcc cacgcccacg
     3721 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3781 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3841 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3901 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     3961 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4021 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4081 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4141 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4201 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4261 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4321 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4381 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4441 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4501 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4561 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4621 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4681 ccgagaatat catccacctg tttaccctga cccggctggg agcccctgcc gccttcaagt
     4741 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4801 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4861 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccgacggc tctgaattcg
     4921 agagccctaa gaagaaaaga aaggtgagcg gaggctctag cggcggaagc accctgaaca
     4981 ttgaagacga gtatagactg catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca
     5041 cctggctgtc cgactttccc caggcctggg ccgagacagg aggaatgggc ctggccgtgc
     5101 ggcaggcacc cctgatcatc cctctgaagg ccacctctac acccgtgagc atcaagcagt
     5161 accctatgtc tcaggaggcc agactgggca tcaagcctca catccagagg ctgctggacc
     5221 agggcatcct ggtgccatgc cagagcccct ggaacacacc actgctgccc gtgaagaagc
     5281 caggcaccaa tgactataga cccgtgcagg atctgagaga ggtgaacaag agggtggagg
     5341 atatccaccc caccgtgccc aacccttaca atctgctgtc cggcctgccc ccttctcacc
     5401 agtggtatac agtgctggac ctgaaggatg ccttcttttg tctgagactg caccctacca
     5461 gccagccact gttcgccttt gagtggaggg accctgagat gggcatctct ggccagctga
     5521 cctggacacg cctgcctcag ggcttcaaga atagcccaac actgtttaac gaggccctgc
     5581 accgcgacct ggcagatttc cggatccagc acccagatct gatcctgctg cagtacgtgg
     5641 acgatctgct gctggccgcc accagcgagc tggattgcca gcagggaaca cgcgccctgc
     5701 tgcagaccct gggaaacctg ggatataggg catccgccaa gaaggcccag atctgtcaga
     5761 agcaggtgaa gtacctgggc tatctgctga aggagggcca gagatggctg acagaggcca
     5821 ggaaggagac agtgatgggc cagccaacac ccaagacccc aagacagctg agggagttcc
     5881 tgggcaaagc aggattttgc aggctgttca tcccaggatt cgcagagatg gcagcacctc
     5941 tgtacccact gaccaagccg ggcaccctgt ttaattgggg ccctgaccag cagaaggcct
     6001 atcaggagat caagcaggcc ctgctgacag caccagccct gggcctgcca gacctgacca
     6061 agcctttcga gctgtttgtg gatgagaagc agggctacgc caagggcgtg ctgacccaga
     6121 agctgggacc atggagacgg cccgtggcct atctgtccaa gaagctggac ccagtggcag
     6181 caggatggcc accatgcctg aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg
     6241 gcaagctgac catgggacag ccactggtca tcctggcacc acacgcagtg gaggccctgg
     6301 tgaagcagcc tccagatcgc tggctgtcta acgcccggat gacacactac caggccctgc
     6361 tgctggacac cgatcgcgtg cagtttggcc ctgtggtggc cctgaatcca gccaccctgc
     6421 tgcctctgcc agaggagggc ctgcagcaca actgtctgga catcctggca gaggcacacg
     6481 gaacaaggcc agacctgacc gatcagcccc tgcctgacgc cgatcacaca tggtataccg
     6541 atggaagctc cctgctgcag gagggccaga ggaaggcagg agcagcagtg accacagaga
     6601 cagaagtgat ctgggccaag gccctgccag caggcacatc cgcccagcgg gccgagctga
     6661 tcgccctgac ccaggccctg aagatggccg agggcaagaa gctgaacgtg tacacagact
     6721 ccagatatgc cttcgccacc gcacacatcc acggagagat ctacaggcgc cggggctggc
     6781 tgacctctga gggcaaggag atcaagaaca aggatgagat cctggccctg ctgaaggccc
     6841 tgtttctgcc caagcggctg agcatcatcc actgtcctgg acaccagaag ggacactccg
     6901 ccgaggcaag gggcaatcgg atggccgacc aggccgccag aaaggctgct attactgaaa
     6961 ctcccgacac ttccactctg ctgattgaaa actcctcccc ttccggagga tctagcggag
     7021 gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa agcagcgggg
     7081 gcagcagcgg ggggtcaatg gctgaaaatg gtgataatga aaagatggct gccctggagg
     7141 ccaaaatctg tcatcaaatt gagtattatt ttggcgactt caatttgcca cgggacaagt
     7201 ttctaaagga acagataaaa ctggatgaag gctgggtacc tttggagata atgataaaat
     7261 tcaacaggtt gaaccgtcta acaacagact ttaatgtaat tgtggaagca ttgagcaaat
     7321 ccaaggcaga actcatggaa atcagtgaag ataaaactaa aatcagaagg tctccaagca
     7381 aacccctacc tgaagtgact gatgagtata aaaatgatgt aaaaaacaga tctgtttata
     7441 ttaaaggctt cccaactgat gcaactcttg atgacataaa agaatggtta gaagataaag
     7501 gtcaagtact aaatattcag atgagaagaa cattgcataa agcatttaag ggatcaattt
     7561 ttgttgtgtt tgatagcatt gaatctgcta agaaatttgt agagacccct ggccagaagt
     7621 acaaagaaac agacctgcta atacttttca aggacgatta ctttgccaaa aaaaatgaat
     7681 ctggcggctc aaaaagaacc gccgacggca gcgaattcga gtctcccaag aagaagagga
     7741 aagtcggctc tggccctgcc gctaagagag tgaagctgga ctaaccggtc atcatcacca
     7801 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7861 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7921 ttcctaataa aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7981 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8041 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     8101 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     8221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8281 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8941 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9121 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     9181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10261 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
    10321 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    10381 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    10441 aggcttgacc gacaattgca tgaagaatct gct
//
