LOCUS       pGP-Tn7-PrpsM-MCS_(no_RBS)_Addgene      6657 bp    DNA     circular SYN 23-AUG-2012
DEFINITION  Cloning and delivery vector pGP-Tn7-Cm, complete sequence.
ACCESSION   JQ429759
VERSION     JQ429759.1
KEYWORDS    .
SOURCE      Cloning and delivery vector pGP-Tn7-Cm (unknown)
  ORGANISM  Cloning and delivery vector pGP-Tn7-Cm
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Crepin,S., Harel,J. and Dozois,C.M.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
   PUBMED   22706059
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Crepin,S., Harel,J. and Dozois,C.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Microbiology and Biothechnology,
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval,
            Quebec H7V 1B7, Canada
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Cloning and delivery vector pGP-Tn7-Cm"
                     /mol_type="other DNA"
                     /db_xref="taxon:1201167"
     repeat_region   189..387
                     /note="Tn7R; right end of Tn7"
     mobile_element  189..2658
                     /mobile_element_type="transposon:mini-Tn7"
     regulatory      436..530
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0 transcriptional terminator"
     regulatory      627..724
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1 transcriptional
                     terminator"
     misc_recomb     765..812
                     /note="FRT; Flp recombinase target site"
     CDS             1000..1659
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyl transferase"
                     /protein_id="AFM78087.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            1000..1659
                     /gene="cat"
     primer          1782..1799
                     /label="9142 pGP-Screen-F"
     misc_recomb     1799..1845
                     /note="FRT; Flp recombinase target site"
     misc_feature    1873..2492
                     /note="MCS; multiple cloning site"
     promoter        1885..2430
                     /label="PrpsM region"
     MCS             2431..2486
     repeat_region   2493..2658
                     /note="Tn7L; left end of Tn7"
     primer          complement(2566..2587)
                     /label="11703 screen R"
     CDS             3159..4019
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AFM78088.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            3159..4019
                     /gene="bla"
     oriT            4493..6220
                     /note="oriT origin of transfer; mob RP4"
     rep_origin      6230..6601
                     /note="R6K origin of replication"
ORIGIN
        1 AATTGTACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG
       61 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTAGA
      121 GGACCAGCCG CGTAACCTGG CAAAATCGGT TACGGTTGAG TAATAAATGG ATGCCCTGCG
      181 TAAGCGGGTG TGGGCGGACA ATAAAGTCTT AAACTGAACA AAATAGATCT AAACTATGAC
      241 AATAAAGTCT TAAACTAGAC AGAATAGTTG TAAACTGAAA TCAGTCCAGT TATGCTGTGA
      301 AAAAGCATAC TGGACTTTTG TTATGGCTAA AGCAAACTCT TCATTTTCTG AAGTGCAAAT
      361 TGCCCGTCGT ATTAAAGAGG GGCGTGGGGT TCGAGGTCGA CGGTATCGAT AAGCTAGCTT
      421 AATTAGCTGA GCTTGGACTC CTGTTGATAG ATCCAGTAAT GACCTCAGAA CTCCATCTGG
      481 ATTTGTTCAG AACGCTCGGT TGCCGCCGGG CGTTTTTTAT TGGTGAGAAT CCAAGCTAGA
      541 CTGCGATGAG TGGCAGGGCG GGGCGTAATT TTTTTAAGGC AGTTATTGGT GCCCTTAAAC
      601 GCCTGGGGTA ATGACTCTCT AGCTTGAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG
      661 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC
      721 CGCCGCTAGG AGCTTGCGGC CCGGACGATC GAATTGGGGA TCTTGAAGTA CCTATTCCGA
      781 AGTTCCTATT CTCTAGAAAG TATAGGAACT TCAGAGCGCT TTTGAAGCTG ATGTGCTTAA
      841 AAACTTACTC AATGGAATAA TTCTAGATGG GCCAACTTTT GGCGAAAATG AGACGTTGAT
      901 CGGCACGTAA GAGGTTCCAA CTTTCACCAT AATGAAATAA GATCACTACC GGGCGTATTT
      961 TTTGAGTTAT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA
     1021 TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA
     1081 GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC
     1141 GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG
     1201 AATGCTCATC CGGAGTTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT
     1261 GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT
     1321 GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC
     1381 GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC
     1441 AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC
     1501 GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG
     1561 GCGATTCAGG TTCATCATGC CGTTTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA
     1621 TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAT TTTTTTAAGG CAGTTATTGG
     1681 TGCCCTTAAA CGCCTGGTTG CTACGCCTGA ATAAGTGATA ATAAGCGGAT GAATGGCAGA
     1741 AATTCGAAAG CAAATTCGAC CCATCCATTG CTGTTGACAA AGGGAATCAG GGGATCTTGA
     1801 AGTTCCTATT CCGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCAGAG CGCTTTTGAA
     1861 GCTAATTCGA TCATGCATGA GCTCGCTTAT CGTTGTTGTC GTGATTATGG ACTTTATGGC
     1921 TCAAGTGCAA ACTCTGATGA TGTCCAGTCA GTATGAGTCT GCATTGAAGA AGGCGAACCT
     1981 GAAAGGCTAC GGCCGTTAAT TGGTCGCCTG AGAAGTTACG GAGAGTAAAA ATGAAAGTTC
     2041 GTGCTTCCGT CAAGAAATTA TGCCGTAACT GCAAAATCGT TAAGCGTGAT GGTGTCATCC
     2101 GTGTGATTTG CAGTGCCGAG CCGAAGCATA AACAGCGCCA AGGCTGATTT TTTCGCATAT
     2161 TTTTCTTGCA AAGTTGGGTT GAGCTGGCTA GATTAGCCAG CCAATCTTTT GTATGTCTGT
     2221 ACGTTTCCAT TTGAGTATCC TGAAAACGGG CTTTTCAGCA TGGTACGTAC ATATTAAATA
     2281 GTAGGAGTGC ATAGTGGCCC GTATAGCAGG CATTAACATT CCTGATCAGA AACACGCCGT
     2341 GATCGCGTTA ACTTCGATCT ACGGTGTCGG CAAGACCCGT TCTAAAGCCA TCCTGGCTGC
     2401 AGCGGGTATC GCTGAAAATG TTAAGATCCT CGGTACCCGG GCGATATCTA GAGACGCGTA
     2461 CCAGGCATGC AAGCTTGGCG GCCGCGCAGG CCAACCAGAT AAGTGAAATC TAGTTCCAAA
     2521 CTATTTTGTC ATTTTTAATT TTCGTATTAG CTTACGACGC TACACCCAGT TCCCATCTAT
     2581 TTTGTCACTC TTCCCTAAAT AATCCTTAAA AACTCCATTT CCACCCCTCC CAGTTCCCAA
     2641 CTATTTTGTC CGCCCACAGC GGGGCATTTT TCTTCCTGTT ATGTTTGGGC GCTGCATTAA
     2701 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
     2761 CTCACTGACC CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
     2821 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
     2881 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
     2941 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
     3001 GGACTATAAA CTCGACCTGC AGATCTGCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
     3061 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
     3121 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
     3181 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
     3241 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
     3301 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
     3361 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC
     3421 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
     3481 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
     3541 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
     3601 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
     3661 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT
     3721 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
     3781 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
     3841 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG
     3901 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
     3961 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
     4021 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTT
     4081 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT
     4141 ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC
     4201 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG
     4261 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC AAGGAGATGG
     4321 CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA
     4381 TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG
     4441 CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCCAGC
     4501 CGACCAGGCT TTCCACGCCC GCGTGCCGCT CCATGTCGTT CGCGCGGTTC TCGGAAACGC
     4561 GCTGCCGCGT TTCGTGATTG TCACGCTCAA GCCCGTAGTC CCGTTCGAGC GTCGCGCAGA
     4621 GGTCAGCGAG GGCGCGGTAG GCCCGATACG GCTCATGGAT GGTGTTTCGG GTCGGGTGAA
     4681 TCTTGTTGAT GGCGATATGG ATGTGCAGGT TGTCGGTGTC GTGATGCACG GCACTGACGC
     4741 GCTGATGCTC GGCGAAGCCA AGCCCAGCGC AGATGCGGTC CTCAATCGCG CGCAACGTCT
     4801 CCGCGTCGGG CTTCTCTCCC GCGCGGAAGC TAACCAGCAG GTGATAGGTC TTGTCGGCCT
     4861 CGGAACGGGT GTTGCCGTGC TGGGTCGCCA TCACCTCGGC CATGACAGCG GGCAGGGTGT
     4921 TTGCCTCGCA GTTCGTGACG CGCACGTGAC CCAGGCGCTC GGTCTTGCCT TGCTCGTCGG
     4981 TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT
     5041 TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC
     5101 GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC
     5161 AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG
     5221 AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG
     5281 TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC
     5341 GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG
     5401 CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC
     5461 TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG
     5521 AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT
     5581 ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC
     5641 CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA
     5701 ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCTGCT TCCCTGCTGT TTTGTGGAAT
     5761 ATCTACCGAC TGGAAACAGG CAAATGCAGG AAATTACTGA ACTGAGGGGA CAGGCGAGAG
     5821 ACGATGCCAA AGAGCTACAC CGACGAGCTG GCCGAGTGGG TTGAATCCCG CGCGGCCAAG
     5881 AAGCGCCGGC GTGATGAGGC TGCGGTTGCG TTCCTGGCGG TGAGGGCGGA TGTCGAGGCG
     5941 GCGTTAGCGT CCGGCTATGC GCTCGTCACC ATTTGGGAGC ACATGCGGGA AACGGGGAAG
     6001 GTCAAGTTCT CCTACGAGAC GTTCCGCTCG CACGCCAGGC GGCACATCAA GGCCAAGCCC
     6061 GCCGATGTGC CCGCACCGCA GGCCAAGGCT GCGGAACCCG CGCCGGCACC CAAGACGCCG
     6121 GAGCCACGGC GGCCGAAGCA GGGGGGCAAG GCTGAAAAGC CGGCCCCCGC TGCGGCCCCG
     6181 ACCGGCTTCA CCTTCAACCC AACACCGGAC AAAAAGGATC CAGCAGTTCA ACCTGTTGAT
     6241 AGTACGTACT AAGCTCTCAT GTTTCACGTA CTAAGCTCTC ATGTTTAACG TACTAAGCTC
     6301 TCATGTTTAA CGAACTAAAC CCTCATGGCT AACGTACTAA GCTCTCATGG CTAACGTACT
     6361 AAGCTCTCAT GTTTCACGTA CTAAGCTCTC ATGTTTGAAC AATAAAATTA ATATAAATCA
     6421 GCAACTTAAA TAGCCTCTAA GGTTTTAAGT TTTATAAGAA AAAAAAGAAT ATATAAGGCT
     6481 TTTAAAGCTT TTAAGGTTTA ACGGTTGTGG ACAACAAGCC AGGGATGTAA CGCACTGAGA
     6541 AGCCCTTAGA GCCTCTCAAA GCAATTTTCA GTGACACAGG AACACTTAAC GGCTGACATG
     6601 GCCTTAGAAG GCGTTTTAGA AGCCCTAATG GATGGAGTCA AAACAAACTA GCGATCG
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