LOCUS       PP589922                7653 bp    DNA     circular SYN 16-JUN-2024
DEFINITION  Vector pJBM3, complete sequence.
ACCESSION   PP589922
VERSION     PP589922.1
KEYWORDS    .
SOURCE      Vector pJBM3
  ORGANISM  Vector pJBM3
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Mcdonald,J.B., Wade,B., Andrews,D.M., Van,T.T.H. and Moore,R.J.
  TITLE     Development of tools for the genetic manipulation of Campylobacter,
            with a focus on the N-glycosylation system of Campylobacter
            hepaticus, an emerging pathogen of poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Mcdonald,J.B., Wade,B., Andrews,D.M., Van,T.T.H. and Moore,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2024) Science, RMIT, 289 McKimmies Rd, Bundoora,
            VIC 3083, Australia
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler pipeline for bacterial genomes
                                     v. Galaxy Version 0.5.0+galaxy1
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Vector pJBM3"
                     /mol_type="other DNA"
                     /db_xref="taxon:3161131"
     rep_origin      1..88
     CDS             330..1367
                     /codon_start=1
                     /transl_table=11
                     /product="replication intiation protein"
                     /protein_id="XBR10389.1"
                     /translation="MSEIVKYHNDFNKIQLPSFTEQEQNLLFLIFARIKEKGIDNVIN
                     LYANDFKINEKLSNSREYLTENINSLKYKFFKANFKQIIETKTEVIHKYVNLFETMEI
                     HYVKRNPDDGYDESTLFNRITLKINPDFAYLVNQLTTNFTAFELEEFIALSGKYAKTL
                     YRLLKQFRTTGKARFEWDEFCKVMDIPQDYRQSEVDKWILKPAIKELSKERNLFDQIR
                     VPFKNLAYEKEKTAGRGRGGKVSGISFTFKPENIQMQKLENESQKIMSDEQKYLKILN
                     NMKLNQVRFDYNDKLWQFNDFDFDEFKIIAIELVRDEYENLNFGNHMHFNAKNQEQFF
                     KMIDTFRKGIR"
     gene            2585..3445
                     /gene="blaTEM"
                     /allele="blaTEM-116"
     CDS             2585..3445
                     /gene="blaTEM"
                     /allele="blaTEM-116"
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-116"
                     /protein_id="XBR10390.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      3616..4204
     CDS             complement(4752..5546)
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="XBR10391.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             5893..6165
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="XBR10392.1"
                     /translation="MQKQYPEVYSLEESLAILKKYKDDLTKEQYENIKSNIGTHAIES
                     IYLNELDIIMLVKKNVYGLSADEIIAEYKKKGFVEYVKSCHRSLQS"
     CDS             6131..7405
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="XBR10393.1"
                     /translation="MSKVATAHCNPKKQPALNHNDRTNDNAKTITKELTHLNEYSCTS
                     DEVRKNIERLYKKAYENFYKYCENKNGLAKSGKPKGLQNFTKKEKCYHEFIYEIGENT
                     TMEQCQELTQKIAELTGFTPLQVVIHRDEVSENAKGEKQTHYHAHAVFFTLDNNGLQL
                     ARREASLNKANLSKIQTLTAQSLKMERGANRYENNEKQPQYIQDYKTYAQFKEQEKAL
                     LQRIQEQEHKLTQMALELKKKEKEIQDKAKELKSKENELQAKIEQHEKHIQNLELGHE
                     RALKELTQEFEKRLSLWKNILTFGKHNAKVREDYQLTKNAFLISTDESRREANKELEY
                     LKFEYHKVKDERDNLKTLFEAHKTKNVKLETRLKEIGKWCEKNLSVEQLKEIFPLKAE
                     RIEKELKYQRAFENSFEQTKTKRNDREFGFSR"
ORIGIN      
        1 tattaagggt agaaatggta ggtattaagg gtagaaatgg taggtattaa gggtagaaat
       61 ggtaggtatt aagggtagaa atggtaggta ttaagggtag aaactattga caatctattt
      121 ttaatatgct acaataaaaa atccgccttg agttttcact cttggcggaa atttatcaat
      181 caaggctaac tatgaaacga catagtaagc gttttgtgag attatactta aattttaggt
      241 tttttaagct taaagttgaa gtatttttct aacaaaaccg caggggcaga gaagcccctt
      301 gccttgattg tcttactttg gatatgcaaa tgagcgaaat agttaaatat cacaacgatt
      361 ttaataaaat tcagttgcct agctttacag agcaagaaca aaaccttttg tttttaatct
      421 ttgcaagaat taaagaaaaa ggcatagaca atgtaattaa tctctatgct aatgatttta
      481 aaattaatga aaaactttct aactcaagag aatatttaac agaaaatatc aatagcttaa
      541 aatataaatt tttcaaggca aattttaaac aaatcataga aacaaagaca gaagttatac
      601 acaaatatgt aaatttgttt gaaacaatgg aaatacacta tgttaaaaga aatcctgatg
      661 atggatatga tgaaagcact ttatttaaca gaataaccct aaaaataaac cctgattttg
      721 cttatttagt caatcaacta actactaatt ttacagcttt tgagcttgaa gaatttatag
      781 cccttagcgg taaatatgct aaaacacttt accgcctttt aaagcaattt agaaccacag
      841 gcaaagcacg atttgagtgg gacgagtttt gtaaagttat ggatatacca caagattata
      901 gacaaagcga agttgataaa tggattttaa aacctgctat caaagaactt tctaaagagc
      961 gcaatctatt tgaccaaatt agagtgcctt ttaaaaatct tgcttatgaa aaagaaaaaa
     1021 ccgcagggcg tgggcgtggc ggtaaagttt caggtattag ctttactttt aaacctgaaa
     1081 atatccaaat gcaaaagcta gaaaatgaaa gtcaaaaaat aatgagcgat gagcaaaaat
     1141 atttaaagat tttaaacaat atgaaactta atcaagttag atttgattat aatgacaagc
     1201 tttggcaatt taacgatttt gattttgatg aatttaaaat tattgcaata gagcttgtaa
     1261 gagatgaata cgagaattta aactttggaa atcatatgca ctttaatgct aaaaatcaag
     1321 agcagttttt taaaatgatt gatactttta gaaaaggtat taggtaaaaa tttgctataa
     1381 ttatatcttt aaaggataca agctatccgc cacttgtgcc aagtgtcgag cttgtgcaag
     1441 tttttatgct ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc
     1501 ctgaaatcct tacatacggc gatatcttct atataagcgt accggttcca attttttcgc
     1561 agtttaactt ttccgacgca tttatcgtct tggtagcttt ttaaatatgg cgcttcatag
     1621 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
     1681 tcaaatggtt cgctgggttt atcgatgata agctgtcaaa catgagaatt cccggggatc
     1741 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
     1801 cggtacccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     1861 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1921 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1981 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2041 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2101 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2161 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2221 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2281 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2341 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2401 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     2461 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2521 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2581 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2641 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2701 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2761 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2821 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2881 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2941 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3001 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3061 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3121 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3181 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3241 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3301 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3361 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3421 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3481 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3541 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3721 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3841 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3901 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3961 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4021 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4081 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4141 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4201 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4261 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4321 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4381 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4441 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4501 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4561 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     4621 gctcgaaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
     4681 ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagcttt ttagacatct
     4741 aaatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
     4801 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
     4861 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
     4921 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
     4981 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
     5041 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
     5101 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
     5161 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
     5221 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
     5281 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
     5341 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
     5401 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
     5461 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
     5521 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
     5581 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
     5641 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
     5701 tagtatcgac ggagccgatt ttgaaaccac aattatgata gaatttacaa gctataaggt
     5761 tattgtcctg ggtttcaagc attagtccat gtgcaacccc ttaaccccac taataaaaat
     5821 caactaaaat caataaatga ttttttgatt tttattaagc ttttaaaaca ccaaaaaagg
     5881 aaaaatttaa aaatgcaaaa acaatatccc gaagtgtata gcttagaaga aagccttgca
     5941 atacttaaaa aatataaaga tgatttaacc aaagagcaat acgagaatat taaatcaaac
     6001 ataggaactc acgctattga aagcatttac cttaacgagt tagatattat tatgttagtt
     6061 aaaaaaaatg tatatggttt aagcgctgat gaaatcattg ctgaatataa gaaaaaggga
     6121 tttgtagaat atgtcaaaag ttgccaccgc tcattgcaat cctaaaaaac aacctgcatt
     6181 aaaccacaat gatagaacca acgataatgc taagacaatc actaaagaac ttacgcattt
     6241 aaatgaatac tcttgcacta gcgatgaagt gcgtaagaac atagaaaggc tttataaaaa
     6301 agcttatgaa aatttttata aatattgtga aaataagaat ggtttagcta aaagtggaaa
     6361 gcctaaagga cttcaaaatt ttactaaaaa agaaaaatgc tatcacgagt ttatatacga
     6421 aatcggcgaa aatactacaa tggagcaatg ccaagagctt acgcaaaaaa tcgcagagct
     6481 tacaggattt acacctttac aagttgtaat ccatagagat gaagtaagtg agaatgctaa
     6541 aggggaaaaa caaacccatt atcacgccca cgcggtattt tttacactcg ataacaatgg
     6601 cttacaactt gctagacgtg aagcaagttt gaataaagcc aatcttagca aaatacaaac
     6661 cctaaccgca caaagtttaa aaatggagcg tggagctaat cgctacgaga ataacgaaaa
     6721 gcaacctcaa tacatacaag attataaaac atacgctcaa tttaaagaac aagaaaaagc
     6781 attacttcaa agaatacaag aacaagagca taaattaacg caaatggccc tagaattgaa
     6841 aaaaaaagaa aaagagatac aagacaaggc taaagagtta aaatcgaaag aaaacgaatt
     6901 acaagcgaaa atagagcaac acgaaaaaca tatacagaat ttagaactag gacacgaaag
     6961 agctttaaaa gaacttacac aagagtttga aaagcgttta agcctatgga aaaacattct
     7021 aacctttgga aaacacaacg ccaaagtaag agaagactat cagctaacta aaaatgcttt
     7081 tttaattagc acagatgaaa gcaggagaga agctaacaaa gagcttgaat atttaaaatt
     7141 tgaatatcat aaagtcaaag atgaacgaga taatttaaaa actttgtttg aggcacacaa
     7201 aacaaaaaat gttaaattag aaactcgact aaaagaaata ggcaaatggt gtgaaaaaaa
     7261 tttaagcgtg gagcagttaa aagaaatatt tccattaaaa gccgaaagaa tagaaaaaga
     7321 gcttaaatat caaagagctt ttgaaaattc ttttgaacaa acaaaaacta aaagaaacga
     7381 tagagagttt gggtttagca gatagttttc ttttttggtg ctttagcaaa tcgcacctgt
     7441 taaggtgcta tgatttgcga aaagggggtg cgggggctgt ctgagtgcgt agcacggaag
     7501 acggacaaag cccaccgccc tagaaaaaaa attactcaac tttttttgtt ttttctctcg
     7561 cgcgcgtacg cgtacgcgat tttttttatt ttttatatat ttttcttatt tttagggggc
     7621 tttctaagcc ctatatgatg ggggctagcg aaa
//

