LOCUS       aTY50_pPGK1_Pcad       12645 bp DNA     circular SYN 07-AUG-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12645
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    16..543
                     /label=pPGK(MTK)
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    16..515
                     /label=PGK promoter
                     /label=nonstandard type: Feature
                     /note="color: #------"
     promoter        16..515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(208..213)
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------; direction: LEFT"
     misc_feature    386..391
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    398..403
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    410..415
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     primer_bind     544..570
                     /label=Pcad_3a_P01_F
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCATATGGAGCTTCTTAGTGGGCCTCACGC; added: 
                     2020-12-07"
     CDS             548..3010
                     /codon_start=1
                     /label=mCdh3(Pcad)
                     /note="color: #993366"
                     /translation="ELLSGPHAFLLLLLQVCWLRSVVSEPYRAGFIGEAGVTLEVEGTD
                     LEPSQVLGKVALAGQGMHHADNGDIIMLTRGTVQGGKDAMHSPPTRILRRRKREWVMPP
                     IFVPENGKGPFPQRLNQLKSNKDRGTKIFYSITGPGADSPPEGVFTIEKESGWLLLHMP
                     LDREKIVKYELYGHAVSENGASVEEPMNISIIVTDQNDNKPKFTQDTFRGSVLEGVMPG
                     TSVMQVTATDEDDAVNTYNGVVAYSIHSQEPKEPHDLMFTIHKSTGTISVISSGLDREK
                     VPEYRLTVQATDMDGEGSTTTAEAVVQILDANDNAPEFEPQKYEAWVPENEVGHEVQRL
                     TVTDLDVPNSPAWRATYHIVGGDDGDHFTITTHPETNQGVLTTKKGLDFEAQDQHTLYV
                     EVTNEAPFAVKLPTATATVVVHVKDVNEAPVFVPPSKVIEAQEGISIGELVCIYTAQDP
                     DKEDQKISYTISRDPANWLAVDPDSGQITAAGILDREDEQFVKNNVYEVMVLATDSGNP
                     PTTGTGTLLLTLTDINDHGPIPEPRQIIICNQSPVPQVLNITDKDLSPNSSPFQAQLTH
                     DSDIYWMAEVSEKGDTVALSLKKFLKQDTYDLHLSLSDHGNREQLTMIRATVCDCHGQV
                     FNDCPRPWKGGFILPILGAVLALLTLLLALLLLVRKKRKVKEPLLLPEDDTRDNVFYYG
                     EEGGGEEDQDYDITQLHRGLEARPEVVLRNDVVPTFIPTPMYRPRPANPDEIGNFIIEN
                     LKAANTDPTAPPYDSLLVFDYEGSGSDAASLSSLTTSASDQDQDYNYLNEWGSRFKKLA
                     DMYGGGEDD"
     primer_bind     complement(593..614)
                     /label=Pcad_3a_P01_R
                     /note="color: black; sequence: 
                     GAACGTCTCAACGCTGCGTAGCCAGCAAAC; added: 2020-12-07"
     primer_bind     607..633
                     /label=Pcad_3a_P02_F
                     /note="color: black; sequence: 
                     GAACGTCTCAGCGTTGTCTCCGAGCCCTACCGAGC; added: 2020-12-07"
     misc_feature    613
                     /label=G->T
                     /note="color: #a6acb3"
     primer_bind     complement(805..832)
                     /label=Pcad_3a_P02_R
                     /note="color: black; sequence: 
                     GAACGTCTCTTCTTAAGATGCGGGTGGGTGGGGAG; added: 2020-12-07"
     primer_bind     825..854
                     /label=Pcad_3a_P03_F
                     /note="color: black; sequence: 
                     GAACGTCTCTAAGAAGACGAAAGAGAGAGTGGGTGATGC; added: 2020-12-07"
     misc_feature    829
                     /label=G->A
                     /note="color: #a6acb3"
     primer_bind     complement(946..965)
                     /label=PcadRv3
                     /note="color: black; sequence: caggccctgtgatgctgtag"
     primer_bind     complement(1644..1674)
                     /label=Pcad_3a_P03_R
                     /note="color: black; sequence: 
                     GAACGTCTCGTTTCTGGGTGAGTGGTGATGGTGAAATGGT; added: 
                     2020-12-07"
     primer_bind     complement(1660..1679)
                     /label=PcadRv2
                     /note="color: black; sequence: ggttggtctctgggtgagtg"
     primer_bind     1668..1697
                     /label=Pcad_3a_P04_F
                     /note="color: black; sequence: 
                     GAACGTCTCAGAAACCAACCAAGGCGTCCTGACAACCA; added: 2020-12-07"
     misc_feature    1672
                     /label=G->A
                     /note="color: #a6acb3"
     primer_bind     complement(2383..2402)
                     /label=PcadRv1
                     /note="color: black; sequence: ccctgttgccatggtcagac"
     primer_bind     complement(2986..3017)
                     /label=Pcad_3a_P04_R
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAAGAACCGTCATCCTCACCGCCACCATACATG; added: 
                     2020-12-07"
     CDS             3004..3010
                     /codon_start=1
                     /label=E-cadherin
                     /note="color: #ff0000"
                     /translation="G"
     misc_feature    3017..3634
                     /label=stop_IRES_start
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    3038..3611
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     3637..3663
                     /label=PuroR_4a_P01_F
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAATCCACCGAGTACAAGCCCACGGTGCG; added: 
                     2020-12-07"
     CDS             3641..4237
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3641..4234
                     /label=puroR
                     /note="color: #004080; direction: RIGHT"
     primer_bind     4004..4021
                     /label=196
                     /note="color: black; sequence: TGGTTCCTGGCCACCGTC"
     primer_bind     complement(4216..4241)
                     /label=PuroR_4a_P01_R
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAGCCATTAGGCACCGGGCTTGCGGGTC; added: 
                     2020-12-07"
     misc_feature    4238..4241
                     /label=BsaI
                     /label=nonstandard type: Feature
                     /note="color: #c6c9d1"
     3'UTR           4242..4780
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    4252..4840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4723..4734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4722"
                     /translation="IEGR"
     misc_feature    complement(4781..4784)
                     /label=Bbs1
                     /label=nonstandard type: Feature
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    complement(4787..4792)
                     /label=Bbs1
                     /label=nonstandard type: Feature
                     /note="color: #faac61; direction: LEFT"
     primer_bind     complement(4862..4878)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             4969..5202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(5227..5245)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    6046..6809
                     /label=ColE1
                     /label=nonstandard type: Feature
                     /note="color: #------"
     rep_origin      complement(6145..6733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    6810..6957
                     /label=KanR Promoter
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    6958..7773
                     /label=Kanr
                     /label=nonstandard type: Feature
                     /note="color: #------"
     CDS             6958..7773
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6958..7773
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    7774..7800
                     /label=KanR Terminator
                     /label=nonstandard type: Feature
                     /note="color: #------"
     promoter        8040..8369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8220..8355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          9503..9568
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             9698..9718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    10143..10277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             10446..11079
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    11126..11251
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    11743..11976
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             12161..12205
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12488..12605
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 ctgatgagac gaacggggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
       61 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      241 aaatggaagt agcatgtctc actaggctcg tgcagatgga cagcaccgct gagcaatgga
      301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc
      541 cactatggag cttcttagtg ggcctcacgc cttcctgctc ctcctgctcc aggtttgctg
      601 gctacgcagc gttgtctccg agccctaccg agcgggcttc atcggggagg ctggagtgac
      661 cttggaggtg gaaggaactg acctggagcc gagccaagtt ctggggaaag tagccttggc
      721 tggacagggc atgcaccatg cagacaatgg agacatcatt atgctgacta gggggacagt
      781 tcagggaggg aaggatgcga tgcactcccc acccacccgc atcttaagaa gacgaaagag
      841 agagtgggtg atgccaccaa tattcgtccc cgagaatggc aagggtccct tccctcagag
      901 gctgaatcag ctcaaatcta ataaggacag aggcaccaag attttctaca gcatcacagg
      961 gcctggcgca gacagtcccc ccgaaggagt cttcaccata gagaaggagt cgggctggct
     1021 gttgttgcat atgccactgg acagggagaa gattgtcaag tacgagcttt atggccacgc
     1081 tgtatctgag aatggtgcct ctgtagagga gcccatgaac atatccatca ttgtgacaga
     1141 ccagaatgac aacaagccca agttcactca agacaccttc agagggagtg ttctggaggg
     1201 agtaatgcct ggcacttctg tgatgcaggt gacagccaca gatgaggacg atgctgtcaa
     1261 cacttacaat ggggtggtgg cttactccat ccatagccaa gagccgaagg agccacacga
     1321 cctcatgttc accatccata aaagcacggg aaccattagc gtcatatcca gtggcctgga
     1381 ccgagagaaa gtccctgagt acagactgac cgtccaggcc acagacatgg atggagaggg
     1441 ctctaccacg acggcagagg ccgttgtgca aatccttgat gccaacgata acgctccgga
     1501 gtttgagccg cagaagtatg aggcttgggt gcctgagaac gaagtgggcc atgaggtaca
     1561 gaggctgaca gtgactgatc tcgatgtccc caactcgcca gcgtggcgtg ccacctacca
     1621 catcgtggga ggtgatgatg gggaccattt caccatcacc actcacccag aaaccaacca
     1681 aggcgtcctg acaaccaaga agggtttgga ttttgaggct caggaccaac acaccctgta
     1741 tgtagaagtg accaacgagg ctccctttgc agtgaagctc ccgacagcca ctgccaccgt
     1801 ggtggtccat gtgaaagatg tcaacgaagc ccctgtgttt gttccacctt ccaaggtcat
     1861 tgaggcccag gaaggcatct ctattgggga actggtctgc atctataccg cacaggaccc
     1921 agacaaggag gaccagaaga tcagctacac catctcgaga gatccagcca actggcttgc
     1981 tgtggaccca gacagtggtc agataactgc cgcaggcatc ttggatcgtg aggacgagca
     2041 gtttgtgaaa aacaatgtct acgaagtcat ggttttggcc acagacagtg gaaaccctcc
     2101 caccaccggc actgggaccc tcctgcttac acttactgac atcaacgacc atggcccgat
     2161 ccctgaaccc aggcagatca tcatctgtaa ccaaagccct gtgcctcaag tgctgaacat
     2221 cactgacaag gacctgtccc ccaactcctc ccctttccag gcccagctaa cacatgactc
     2281 agatatctac tggatggcag aagtcagcga gaaaggagac accgtggcct tgtccctgaa
     2341 gaagttcctg aaacaagaca cgtatgactt gcatctttct ttgtctgacc atggcaacag
     2401 ggaacagcta accatgatca gggccactgt gtgtgactgc catggccaag tgttcaatga
     2461 ctgccccaga ccctggaagg gtggtttcat cctccccatc ctgggtgctg tcctggcact
     2521 gctgaccctt ctactggcac tcctcctgtt ggtgaggaag aagaggaagg tcaaagagcc
     2581 ccttctgctc ccagaagatg acacgcgaga caatgtcttc tattatggag aagagggtgg
     2641 tggtgaagag gaccaggact atgacatcac ccaactccac cggggactgg aggccaggcc
     2701 tgaggtggtt ctccgaaacg atgtagtgcc aaccttcatc cccaccccca tgtaccgacc
     2761 ccggcccgcc aacccagatg aaatcgggaa cttcatcatc gagaacctga aggctgccaa
     2821 cactgaccct actgccccgc cctacgactc cctgctggtt tttgactacg agggcagcgg
     2881 ctctgatgcc gcctccctga gctccctcac cacctccgcc tccgaccagg atcaggacta
     2941 caactacctt aacgagtggg gaagtcgatt caagaaactg gcggacatgt atggtggcgg
     3001 tgaggatgac ggttcttaag atccttgact tgcggccccc ctctccctcc ccccccccta
     3061 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3121 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3181 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3241 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3301 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3361 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3421 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3481 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3541 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3601 cacgatgata aatgagcgct ggcggaccac catgggatcc accgagtaca agcccacggt
     3661 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3721 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3781 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3841 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3901 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3961 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4021 cggcgtgtcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4081 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct
     4141 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4201 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaatgg cgcttatcga taatcaacct
     4261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4801 cctcagacga gtcggatctc cctttgggcc gcctccccgc atgctgcgtc tcaagcatac
     4861 acgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt
     4921 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4981 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtttctc tggttagacc
     5041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5161 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
     5221 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
     5281 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
     5341 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
     5401 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
     5461 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     5521 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     5581 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     5641 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     5701 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     5761 ctctcagtac aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5821 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5881 accatctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5941 tgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6001 tagacgtcag gtggcacttt tcggggaaat gtgccgagcg gccgcacggt tatccacaga
     6061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6781 aaactcacgt taagggattt tggtcatgac tgatccttca actcagcaaa agttcgattt
     6841 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     6901 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     6961 agccatattc aacgggaaac gtcttgctcc aggccgcgat taaattccaa catggatgct
     7021 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     7081 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     7141 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     7201 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     7261 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     7321 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     7381 aacagcgatc gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     7441 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     7501 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     7561 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     7621 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     7681 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     7741 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     7801 gcggccgcca attttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7861 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7921 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7981 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
     8041 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8101 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8161 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8221 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8281 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8341 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
     8401 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     8461 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     8521 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     8581 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     8641 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
     8701 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
     8761 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
     8821 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
     8881 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
     8941 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
     9001 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
     9061 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
     9121 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
     9181 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
     9241 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
     9301 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
     9361 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
     9421 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     9481 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     9541 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     9601 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     9661 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     9721 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     9781 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     9841 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     9901 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     9961 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
    10021 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
    10081 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    10141 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10261 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10321 ccccataccc tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat
    10381 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10441 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
    10501 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
    10561 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
    10621 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
    10681 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
    10741 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
    10801 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
    10861 cagatcctgc atataagcag ctgctttttg cctgtactgg gactctctgg ttagaccaga
    10921 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10981 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    11041 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    11101 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    11161 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    11221 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    11281 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    11341 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    11401 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    11461 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    11521 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    11581 acagcaagcg gccggtgatc ttcagacctg gacgatatat atgagggaca attggagaag
    11641 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    11701 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    11761 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    11821 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    11881 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    11941 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    12001 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    12061 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    12121 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    12181 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    12241 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    12301 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    12361 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
    12421 cagagacaga tccattcgat tagtgaacgg atctcgacgg tcgccaaatg gcagtattca
    12481 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    12541 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    12601 attttcgggt ttattacagg gacagcagag atccagtttg gccct
//
