LOCUS       aTY173_pPGK1_mTa        9668 bp DNA     circular SYN 07-AUG-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9668
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    16..543
                     /label=pPGK(MTK)
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    16..515
                     /label=PGK promoter
                     /label=nonstandard type: Feature
                     /note="color: #------"
     promoter        16..515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(208..213)
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------; direction: LEFT"
     misc_feature    386..391
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    398..403
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    410..415
                     /label=SP1 Binding Site
                     /label=nonstandard type: Feature
                     /note="color: #------"
     CDS             545..1256
                     /codon_start=1
                     /label=mTagBFP2
                     /note="color: #3366ff"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1258..1261
                     /label=BsaI
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    1262..1264
                     /label=Stop codon
                     /note="color: #a6acb3"
     regulatory      1270..1865
                     /label=WPRE
                     /note="color: #ffffff"
                     /regulatory_class="recoding_stimulatory_region"
     misc_feature    1275..1863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(1746..1757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 1745"
                     /translation="IEGR"
     misc_feature    complement(join(1804..1807,1810..1815))
                     /label=Bbs1
                     /label=nonstandard type: Feature
                     /note="This reverse directional feature has 2 segments:
                        1: 1804 .. 1807 / #------
                        2: 1810 .. 1815 / #------"
     primer_bind     complement(1885..1901)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             1992..2225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(2250..2268)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    3069..3832
                     /label=ColE1
                     /label=nonstandard type: Feature
                     /note="color: #------"
     rep_origin      complement(3168..3756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    3833..3980
                     /label=KanR Promoter
                     /label=nonstandard type: Feature
                     /note="color: #------"
     misc_feature    3981..4796
                     /label=Kanr
                     /label=nonstandard type: Feature
                     /note="color: #------"
     CDS             3981..4796
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3981..4796
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    4797..4823
                     /label=KanR Terminator
                     /label=nonstandard type: Feature
                     /note="color: #------"
     promoter        5063..5392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5243..5378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          6526..6591
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             6721..6741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    7166..7300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             7469..8102
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    8149..8274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8766..8999
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             9184..9228
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9511..9628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 ctgatgagac gaacggggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
       61 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      241 aaatggaagt agcatgtctc actaggctcg tgcagatgga cagcaccgct gagcaatgga
      301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc
      541 cactatggtt tcaaagggtg aagaattgat caaagaaaac atgcatatga aattatatat
      601 ggaaggtaca gttgacaacc atcattttaa gtgtacatct gaaggagaag gtaagcctta
      661 cgagggaact caaactatga gaataaaagt cgtagaaggt ggaccattgc cttttgcttt
      721 tgatatctta gcaacatctt tcttatatgg ttctaaaacc tttattaacc atactcaagg
      781 tatccctgat ttcttcaaac aatctttccc tgaaggattt acttgggaga gggttacaac
      841 atatgaggat ggtggtgttt tgacagcaac ccaggataca tcattgcagg atggatgttt
      901 gatttataat gtaaagatta gaggagtaaa ttttacttct aacggtcctg taatgcaaaa
      961 gaagacttta ggttgggaag ctttcaccga aacattatat ccagcagacg gaggtttaga
     1021 gggaagaaac gatatggctt tgaagttagt cggtggttca cacttaatag ctaacgccaa
     1081 gactacttac aggtctaaga aaccagcaaa gaatttgaaa atgccaggag tttattatgt
     1141 tgactacaga ttggaaagga tcaaggaggc caataacgag acatatgtag agcagcatga
     1201 agtcgccgtc gccagatatt gcgacttacc ttctaagttg ggtcataaat tgaatggatc
     1261 ctaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     1681 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     1741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     1801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     1861 cgcatgctgc gtctcaagca tacacgatac cgtcgacctc gagggaatta attcgagctc
     1921 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1981 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2041 tactgggttt ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2221 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     2281 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     2341 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     2401 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     2461 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     2521 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2581 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     2641 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     2701 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     2761 aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc gcatagttaa
     2821 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2881 catccgctta cagacaagct gtgaccatct ccgggagctg catgtgtcag aggttttcac
     2941 cgtcatcacc gaaacgcgcg agatgaaagg gcctcgtgat acgcctattt ttataggtta
     3001 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgccga
     3061 gcggccgcac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactgatcct
     3841 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
     3901 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     3961 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tccaggccgc
     4021 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     4081 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     4141 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     4201 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     4261 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     4321 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     4381 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgcctcgct caggcgcaat
     4441 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     4501 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
     4561 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     4621 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     4681 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     4741 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     4801 aattggttaa ttggttgtaa cacgcggccg ccaatttttt acggttcctg gccttttgct
     4861 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4921 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4981 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5041 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5101 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5161 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5221 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5341 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     5401 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     5461 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     5521 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     5581 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     5641 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     5701 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     5761 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     5821 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     5881 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     5941 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     6001 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     6061 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     6121 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     6181 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     6241 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     6301 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     6361 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     6421 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     6481 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     6541 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     6601 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     6661 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     6721 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     6781 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     6841 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     6901 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     6961 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     7021 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     7081 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     7141 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     7201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7261 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     7321 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     7381 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     7441 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     7501 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     7561 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7621 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     7681 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     7741 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     7801 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     7861 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     7921 tgggactctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8101 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8161 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8221 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8281 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8341 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8401 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8461 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8521 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8581 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
     8641 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8701 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8761 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8821 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8881 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8941 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9001 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9061 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9121 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9181 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9241 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9301 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9361 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     9421 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     9481 cggtcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9541 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9601 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     9661 ttggccct
//
