LOCUS       pDGB3_α2_4xCBS:mDFR:Rep 8318 bp ds-DNA     circular     01-AUG-2024
DEFINITION  (GB_UD_B061,GB_UD_6A3F,GB_UD_A6AA,GB0037)pDGB3_alpha2.
ACCESSION   GB_UA_A07D
VERSION     GB_UA_A07D
COMMENT     Imported using the Genbank importer. File name:
            GB_UA_A07D.8omb_i0r.gb
FEATURES             Location/Qualifiers
     misc_feature    5..198
                     /label="CBS operator (4xMRE repeats)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    203..357
                     /label="Sl minimal DFR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    359..1237
                     /label="BeYDV's RepA (C1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    706..706
                     /label="puntual"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    950..1035
                     /label="Intron"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1015..1449
                     /label="Partial BeYDV's Rep"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     terminator      1450..1945
                     /label="Tnos"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /vntifkey=43
     misc_feature    1534..1534
                     /label="T>C"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /vntifkey=62
     misc_feature    1963..1987
                     /label="RB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /vntifkey=21
     misc_feature    complement(3049..4049)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="STA region from pVS1 plasmid"
                     /vntifkey=21
     misc_feature    complement(4642..5642)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey=21
     misc_feature    complement(6052..6312)
                     /label="pBR322"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="bom site from pBR322"
                     /vntifkey=21
     rep_origin      complement(6452..6732)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pBR322 origin of replication"
                     /vntifkey=33
     CDS             complement(7027..7839)
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="kanamycin resistance gene"
                     /vntifkey=4
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    8272..8297
                     /label="LB"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /vntifkey=21
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaaat
      361 gccttctgct agtaagaact tcagactcca atctaaatat gttttcctta cctatcccaa
      421 gtgctcatct caaagagatg atttattcca gtttctctgg gagaaactca caccttttct
      481 tattttcttc cttggtgttg cttctgagct tcatcaagat ggcactaccc actatcatgc
      541 tcttctccag cttgataaaa aaccttgtat tagggatcct tcttttttcg attttgaagg
      601 aaatcaccct aatatccagc cagctagaaa ctctaaacaa gtccttgatt acatatcaaa
      661 ggacggagat attaaaacca gaggagattt ccgagatcat aaggtttctc ctcgcaaatc
      721 tgacgcacga tggagaacta ttatccagac tgcaacgtct aaggaggaat atcttgacat
      781 gatcaaggaa gaattccctc atgaatgggc aacaaagctt caatggctgg aatattcagc
      841 caacaaatta ttccctccac aacctgaacc gtatgtgtcg cccttcacag aatcagatct
      901 tcgctgccac gaagatctac actcctggag ggaaacccat ctataccatg taagcataga
      961 cgcttatact tacatacatc ctgtctcata ccaacaagct caatctgacc ttgaatggat
     1021 ggccgattta accaggacaa tggaaggaat ggaatccgac accccagcct ctacatctgc
     1081 ggaccaactc gtaccggaaa gaccacctgg gctagaagtc tcggacgaca caactattgg
     1141 aacggtacca tcgatttcac caactacgat gaacacgcca cctataatat catcgacgac
     1201 atccccttca agttcgtccc attgtggaag caattaatag gttgccagtc tgatttcact
     1261 gtcaacccta aatatggaaa aaagaagaaa ataaaaggtg ggatcccttc tataattctt
     1321 tgcaatcctg acgaagactg gatgttatca atgacaagtc aacagaagga ttactttaaa
     1381 gataattgcg tcacccacta catgtgtgac ggggagactt tttttgctcg ggaatcgtcg
     1441 agtcactgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     1501 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     1561 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     1621 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     1681 actaggataa attatcgcgc dcggtgtcat ctatgttact agatcgggaa ttgccaagct
     1741 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1801 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1921 atgcttcaat aatgggaccg actcgcgctt gagacgaagc tttgacagga tatattggcg
     1981 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     2041 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     2101 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     2161 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     2221 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     2281 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     2341 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     2401 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     2461 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     2521 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     2581 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     2641 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     2701 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     2761 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     2821 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     2881 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     2941 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     3001 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     3061 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     3121 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     3181 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     3241 gcgtatatga tccgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     3301 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cggaacccat ctagcccgcg
     3361 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     3421 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     3481 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     3541 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     3601 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     3661 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     3721 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     3781 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     3841 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     3901 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     3961 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     4021 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     4081 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat agatcgcgca
     4141 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     4201 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     4261 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     4321 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
     4381 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     4441 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     4501 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     4561 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     4621 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     4681 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     4741 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     4801 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     4861 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     4921 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     4981 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     5041 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     5101 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     5161 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     5221 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     5281 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     5341 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     5401 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     5461 aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     5521 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     5581 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     5641 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     5701 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     5761 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     5821 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     5881 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggtgac
     5941 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6001 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     6061 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6121 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     6181 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6241 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6361 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6421 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6481 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6541 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6601 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6661 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6721 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6781 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6841 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6901 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6961 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     7021 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     7081 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     7141 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     7201 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     7261 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     7321 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     7381 ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     7441 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     7501 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     7561 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     7621 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     7681 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
     7741 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     7801 cggccttgag caagacgttt cccgttgaat atggctcata acagaactta ttatttcctt
     7861 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     7921 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     7981 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     8041 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     8101 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     8161 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     8221 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     8281 atattgtggt gtaaacataa caagcttcgt ctcagtca
//