LOCUS       pDGB3_α1_35S:eGFP:T35S_ 8362 bp ds-DNA     circular     01-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label="GTCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    17..41
                     /label="RB"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(69..89)
                     /label="pCambiaR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1103..2103)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2696..3696)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4106..4366)
                     /label="pBR322"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(4506..4786)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(5081..5893)
                     /label="KanR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6324..6343
                     /label="pCambiaF"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    6326..6351
                     /label="LB"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        6372..7398
                     /label="35S"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7400..8128
                     /label="eGFP"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      complement(8133..8358)
                     /label="T35S"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gtcatgagac gaattctgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
       61 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
      121 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
      181 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc
      241 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
      301 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
      361 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
      421 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
      481 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
      541 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
      601 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
      661 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
      721 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
      781 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
      841 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
      901 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
      961 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     1021 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     1081 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     1141 cctctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     1201 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     1261 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatccgat gagtaaataa
     1321 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     1381 ggcaagacga ccatcggaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     1441 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     1501 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     1561 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     1621 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     1681 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     1741 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     1801 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     1861 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     1921 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     1981 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     2041 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     2101 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     2161 ccattaccga gctgctatct gaatagatcg cgcagctacc agagtaaatg agcaaatgaa
     2221 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     2281 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     2341 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     2401 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     2461 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     2521 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     2581 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     2641 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     2701 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     2761 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     2821 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     2881 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     2941 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     3001 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     3061 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     3121 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     3181 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     3241 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     3301 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     3361 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     3421 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     3481 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt
     3541 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     3601 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     3661 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     3721 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     3781 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     3841 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     3901 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     3961 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg
     4021 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     4081 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     4141 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     4201 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4261 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4321 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4561 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4801 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5041 ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca
     5101 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     5161 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     5221 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     5281 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     5341 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     5401 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc
     5461 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     5521 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     5581 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     5641 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     5701 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     5761 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     5821 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt
     5881 gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta tttaaagata
     5941 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
     6001 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
     6061 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
     6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac ataacgaatt
     6361 cgtctcagga gactagagcc aagctgatct cctttgcccc ggagatcacc atggacgact
     6421 ttctctatct ctacgatcta ggaagaaagt tcgacggaga aggtgacgat accatgttca
     6481 ccaccgataa tgagaagatt agcctcttca atttcagaaa gaatgctgac ccacagatgg
     6541 ttagagaggc ctacgcggca ggtctgatca agacgatcta cccgagtaat aatctccagg
     6601 agatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
     6661 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
     6721 ttcaaggctt gcttcataaa ccaaggcaag taatagagat tggagtctct aagaaagtag
     6781 ttcctactga atcaaaggcc atggagtcaa aaattcagat cgaggatcta acagaactcg
     6841 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
     6901 tcttcgtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
     6961 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
     7021 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
     7081 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
     7141 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     7201 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     7261 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     7321 agaggactcc ggtattttta caacaatacc acaacaaaac aaacaacaaa caacattaca
     7381 atttactatt ctagtcgaaa tgGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
     7441 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
     7501 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     7561 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG
     7621 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
     7681 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
     7741 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
     7801 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT
     7861 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA
     7921 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
     7981 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
     8041 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
     8101 GGACGAGCTG TACAAGGACG AGCTGTAAGC TTcggccatg ctagagtccg caaaaatcac
     8161 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     8221 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     8281 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     8341 accaaaatcc agtgacctcg ct
//