LOCUS       pDGB3_α1_CBS-FRT-OCS-Be 9092 bp ds-DNA     circular     01-AUG-2024
DEFINITION  (GB_UD_B061,GB4642,GB_UD_A6AA,GB0037)pDGB3_alpha1.
ACCESSION   GB_UA_7C14
VERSION     GB_UA_7C14
COMMENT     Imported using the Genbank importer. File name:
            GB_UA_7C14.ewya4m_a.gb
FEATURES             Location/Qualifiers
     misc_feature    5..198
                     /label="CBS operator (4x MRE repetitions)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    199..314
                     /label="mini DFR 1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    229..234
                     /label="TATA box"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    265..265
                     /label=+1
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    304..309
                     /label="CNGTTR_(myb\core)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    315..348
                     /label="FRT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    349..1056
                     /label="OCS terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1057..1090
                     /label="FRT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1091..1133
                     /label="mini DFR 2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1135..2013
                     /label="BeYDV's RepA (C1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1482..1482
                     /label="puntual"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1726..1811
                     /label="Intron"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1791..2225
                     /label="Partial BeYDV's Rep"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     terminator      2226..2721
                     /label="Tnos"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /vntifkey=43
     misc_feature    2310..2310
                     /label="T>C"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /vntifkey=62
     misc_feature    2722..2725
                     /label="CGCT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /vntifkey=21
     misc_feature    2726..2729
                     /label="GTCA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /vntifkey=21
     misc_feature    2742..2766
                     /label="RB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /vntifkey=21
     misc_feature    complement(3828..4828)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="STA region from pVS1 plasmid"
                     /vntifkey=21
     misc_feature    complement(5421..6421)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey=21
     misc_feature    complement(6831..7091)
                     /label="pBR322"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="bom site from pBR322"
                     /vntifkey=21
     rep_origin      complement(7231..7511)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin of replication"
                     /vntifkey=33
     CDS             complement(7806..8618)
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="kanamycin resistance gene"
                     /vntifkey=4
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    9051..9076
                     /label="LB"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /vntifkey=21
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttgaagtt cctattctct agaaagtata ggaacttcct gctttaatga
      361 gatatgcgag aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta
      421 aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat
      481 atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt
      541 accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt
      601 atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat
      661 ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta
      721 ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat
      781 aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag
      841 ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac
      901 ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg
      961 ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca
     1021 gggcgaattt tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta
     1081 taggaacttc aaggttaaat ttgctgactc ttttttacct ttcatttctg aaaaatgcct
     1141 tctgctagta agaacttcag actccaatct aaatatgttt tccttaccta tcccaagtgc
     1201 tcatctcaaa gagatgattt attccagttt ctctgggaga aactcacacc ttttcttatt
     1261 ttcttccttg gtgttgcttc tgagcttcat caagatggca ctacccacta tcatgctctt
     1321 ctccagcttg ataaaaaacc ttgtattagg gatccttctt ttttcgattt tgaaggaaat
     1381 caccctaata tccagccagc tagaaactct aaacaagtcc ttgattacat atcaaaggac
     1441 ggagatatta aaaccagagg agatttccga gatcataagg tttctcctcg caaatctgac
     1501 gcacgatgga gaactattat ccagactgca acgtctaagg aggaatatct tgacatgatc
     1561 aaggaagaat tccctcatga atgggcaaca aagcttcaat ggctggaata ttcagccaac
     1621 aaattattcc ctccacaacc tgaaccgtat gtgtcgccct tcacagaatc agatcttcgc
     1681 tgccacgaag atctacactc ctggagggaa acccatctat accatgtaag catagacgct
     1741 tatacttaca tacatcctgt ctcataccaa caagctcaat ctgaccttga atggatggcc
     1801 gatttaacca ggacaatgga aggaatggaa tccgacaccc cagcctctac atctgcggac
     1861 caactcgtac cggaaagacc acctgggcta gaagtctcgg acgacacaac tattggaacg
     1921 gtaccatcga tttcaccaac tacgatgaac acgccaccta taatatcatc gacgacatcc
     1981 ccttcaagtt cgtcccattg tggaagcaat taataggttg ccagtctgat ttcactgtca
     2041 accctaaata tggaaaaaag aagaaaataa aaggtgggat cccttctata attctttgca
     2101 atcctgacga agactggatg ttatcaatga caagtcaaca gaaggattac tttaaagata
     2161 attgcgtcac ccactacatg tgtgacgggg agactttttt tgctcgggaa tcgtcgagtc
     2221 actgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt
     2281 aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt
     2341 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     2401 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     2461 ggataaatta tcgcgcdcgg tgtcatctat gttactagat cgggaattgc caagctaatt
     2521 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2581 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2641 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2701 ttcaataatg ggaccgactc gcgctgtcat gagacgaatt ctgacaggat atattggcgg
     2761 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     2821 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     2881 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     2941 ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     3001 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     3061 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     3121 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     3181 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     3241 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     3301 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     3361 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     3421 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     3481 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     3541 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     3601 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     3661 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     3721 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     3781 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     3841 ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa atcctggccg
     3901 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     3961 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     4021 cgtatatgat ccgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     4081 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc ggaacccatc tagcccgcgc
     4141 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     4201 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     4261 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     4321 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     4381 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     4441 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     4501 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     4561 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     4621 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     4681 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     4741 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     4801 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     4861 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata gatcgcgcag
     4921 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     4981 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     5041 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     5101 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     5161 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5221 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     5281 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     5341 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     5401 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     5461 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     5521 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     5581 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     5641 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     5701 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     5761 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     5821 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     5881 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     5941 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6001 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6061 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6121 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6181 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6241 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     6301 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     6361 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     6421 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     6481 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     6541 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     6601 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     6661 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
     6721 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6781 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     6841 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6901 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7561 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     7801 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     7861 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     7921 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     7981 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     8041 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     8101 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     8161 tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     8221 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     8281 tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca
     8341 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     8401 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     8461 aacaactctg gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg
     8521 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     8581 ggccttgagc aagacgtttc ccgttgaata tggctcataa cagaacttat tatttccttc
     8641 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     8701 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     8761 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
     8821 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     8881 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     8941 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     9001 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     9061 tattgtggtg taaacataac gaattcgtct ca
//