LOCUS       pDGB3_o1_LIR:2nd_intron 9758 bp ds-DNA     circular     01-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label="GTCA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    18..42
                     /label="RB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1104..2104)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2697..3697
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4107..4367)
                     /label="pBR322"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     rep_origin      complement(4507..4787)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(5079..6089)
                     /label="SpmR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        complement(6090..6223)
                     /label="Spm\Promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    6656..6681
                     /label="LB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    6703..7067
                     /label="LIR1 BeYDV"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7068..7130
                     /label="2nd half intron"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             7133..7525
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKDEL*"
     misc_feature    7135..7525
                     /label="3' eGFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7530..7755
                     /label="Term35S"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7756..7914
                     /label="SIR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        7927..8953
                     /label="35S"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             8955..9292
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE"
     misc_feature    8958..9290
                     /label="5' eGFP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    9295..9353
                     /label="1st half intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    9390..9754
                     /label="LIR1 BeYDV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    9402..9697
                     /label="annotated LIR in NCBI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 gtcatgagac cggatcctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
       61 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
      121 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
      181 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
      241 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
      301 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
      361 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
      421 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
      481 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
      541 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
      601 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
      661 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
      721 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
      781 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
      841 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
      901 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
      961 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     1021 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     1081 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     1141 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     1201 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     1261 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatccga tgagtaaata
     1321 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     1381 aggcaagacg accatcggaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     1441 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     1501 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     1561 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     1621 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     1681 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     1741 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     1801 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     1861 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     1921 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     1981 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     2041 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     2101 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     2161 accattaccg agctgctatc tgaatagatc gcgcagctac cagagtaaat gagcaaatga
     2221 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     2281 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     2341 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     2401 gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     2461 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     2521 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     2581 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     2641 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     2701 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     2761 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     2821 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     2881 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     2941 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     3001 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     3061 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     3121 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     3181 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     3241 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     3301 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     3361 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     3421 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     3481 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg
     3541 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     3601 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     3661 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     3721 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     3781 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     3841 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     3901 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     3961 ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct gtaagcggat
     4021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     4081 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     4141 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4201 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4801 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5041 actcacgtta agggattttg gtcatgcatt ctaggtgatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcacc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat aatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     6061 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa
     6121 caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     6181 accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggaacagaa cttattattt
     6241 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     6301 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     6361 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     6421 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     6481 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     6541 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     6601 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     6661 ggatatattg tggtgtaaac ataacggatc cggtctcagg agTAGCAGAA GGCATGTTGT
     6721 TGTGACTCCG AGGGGTTGCC TCAAACTCTA TCTTATAACC GGCGTGGAGG CATGGAGGCA
     6781 GGGGTATTTT GGTCATTTTA ATAGATAGTG GAAAATGACG TGGAATTTAC TTAAAGACGA
     6841 AGTCTTTGCG ACAAGGGGGG GCCCACGCCG AATTTAATAT TACCGGCGTG GCCCCCCCTT
     6901 ATCGCGAGTG CTTTAGCACG AGCGGTCCAG ATTTAAAGTA GAAAATTTCC CGCCCACTAG
     6961 GGTTAAAGGT GTTCACACTA TAAAAGCATA TACGATGTGA TGGTATTTGA TGGAGCGTAT
     7021 ATTGTATCAG GTATTTCCGT TGGATACGAA TTATTCGTAC GACCCTCCTA ATTAACCTGA
     7081 CTCAAAATCT ACCCTCATAA TTGTTGTTTG ATATTGGTCT TGTATTTTGC AGGTGAAGTT
     7141 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG
     7201 CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC
     7261 CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG
     7321 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
     7381 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
     7441 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
     7501 CGAGCTGTAC AAGGACGAGC TGTAAGCTTC GGCCATGCTA GAGTCCGCAA AAATCACCAG
     7561 TCTCTCTCTA CAAATCTATC TCTCTCTATT TTTCTCCAGA ATAATGTGTG AGTAGTTCCC
     7621 AGATAAGGGA ATTAGGGTTC TTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC
     7681 TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC
     7741 AAAATCCAGT GACCTATAAA ATGATTATTT TATGAATATA TTTCATTGTG CAAGTAGATA
     7801 GAAATTACAT ATGTTACATA ACACACGAAA TAAACAAAAA AAGACAATCC AAAAACAAAC
     7861 ACCCCAAAAA AAATAATCAC TTTAGATAAA CTCGTATGAG GAGAGGCACG TTCACGCTgt
     7921 caggagacta gagccaagct gatctccttt gccccggaga tcaccatgga cgactttctc
     7981 tatctctacg atctaggaag aaagttcgac ggagaaggtg acgataccat gttcaccacc
     8041 gataatgaga agattagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
     8101 gaggcctacg cggcaggtct gatcaagacg atctacccga gtaataatct ccaggagatc
     8161 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     8221 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     8281 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaagaa agtagttcct
     8341 actgaatcaa aggccatgga gtcaaaaatt cagatcgagg atctaacaga actcgccgtg
     8401 aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa gaaaatcttc
     8461 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     8521 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     8581 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     8641 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     8701 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     8761 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     8821 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     8881 actccggtat ttttacaaca ataccacaac aaaacaaaca acaaacaaca ttacaattta
     8941 ctattctagt cgaaatgGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
     9001 TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
     9061 GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG
     9121 TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC
     9181 CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG
     9241 AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG AGGTAAGCCA
     9301 TCTTCCTGCT TATTTTTATA ATGAACATAG AAATAGGAAG TTGTGCAGAG AAACGCTGTC
     9361 AGGAGGTGCC AAGCTTGCAT GCCTGCAGGT AGCAGAAGGC ATGTTGTTGT GACTCCGAGG
     9421 GGTTGCCTCA AACTCTATCT TATAACCGGC GTGGAGGCAT GGAGGCAGGG GTATTTTGGT
     9481 CATTTTAATA GATAGTGGAA AATGACGTGG AATTTACTTA AAGACGAAGT CTTTGCGACA
     9541 AGGGGGGGCC CACGCCGAAT TTAATATTAC CGGCGTGGCC CCCCCTTATC GCGAGTGCTT
     9601 TAGCACGAGC GGTCCAGATT TAAAGTAGAA AATTTCCCGC CCACTAGGGT TAAAGGTGTT
     9661 CACACTATAA AAGCATATAC GATGTGATGG TATTTGATGG AGCGTATATT GTATCAGGTA
     9721 TTTCCGTTGG ATACGAATTA TTCGTACGAC CCTCCGCT
//