LOCUS       pDGB3_α2_BeYDV_geminino 9427 bp ds-DNA     circular     01-AUG-2024
DEFINITION  (GB_UD_FC88,GB_UD_100AA,GB_UD_BF3C,GB_UD_BF3F,GB_UD_FC8A)pDGB3_alph
            a2.
ACCESSION   GB_UA_9323
VERSION     GB_UA_9323
COMMENT     Imported using the Genbank importer. File name:
            GB_UA_9323.198b4tzw.gb
FEATURES             Location/Qualifiers
     misc_feature    5..369
                     /label="LIR1 BeYDV"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    370..432
                     /label="2nd half intron"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    433..436
                     /label="END intron"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    437..1162
                     /label="eGFP no ATG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1167..1392
                     /label="Term35S"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1393..1551
                     /label="SIR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        1556..2582
                     /label="35S"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2591..2594
                     /label="START intron"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2595..2653
                     /label="1st half intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2690..3054
                     /label="LIR1 BeYDV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3072..3096
                     /label="RB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /vntifkey=21
     misc_feature    complement(4158..5158)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="STA region from pVS1 plasmid"
                     /vntifkey=21
     misc_feature    complement(5751..6751)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey=21
     misc_feature    complement(7161..7421)
                     /label="pBR322"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="bom site from pBR322"
                     /vntifkey=21
     rep_origin      complement(7561..7841)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pBR322 origin of replication"
                     /vntifkey=33
     CDS             complement(8136..8948)
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="kanamycin resistance gene"
                     /vntifkey=4
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    9381..9406
                     /label="LB"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /vntifkey=21
ORIGIN
        1 ggagtagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc tatcttataa
       61 ccggcgtgga ggcatggagg caggggtatt ttggtcattt taatagatag tggaaaatga
      121 cgtggaattt acttaaagac gaagtctttg cgacaagggg gggcccacgc cgaatttaat
      181 attaccggcg tggccccccc ttatcgcgag tgctttagca cgagcggtcc agatttaaag
      241 tagaaaattt cccgcccact agggttaaag gtgttcacac tataaaagca tatacgatgt
      301 gatggtattt gatggagcgt atattgtatc aggtatttcc gttggatacg aattattcgt
      361 acgaccctcc taattaacct gactcaaaat ctaccctcat aattgttgtt tgatattggt
      421 cttgtatttt gcaggtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      481 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      541 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      601 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      661 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      721 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      781 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      841 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      901 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      961 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1021 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1081 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1141 gctgtacaag gacgagctgt aagcttcggc catgctagag tccgcaaaaa tcaccagtct
     1201 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga
     1261 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     1321 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     1381 atccagtgac ctataaaatg attattttat gaatatattt cattgtgcaa gtagatagaa
     1441 attacatatg ttacataaca cacgaaataa acaaaaaaag acaatccaaa aacaaacacc
     1501 ccaaaaaaaa taatcacttt agataaactc gtatgaggag aggcacgttc aggtaactag
     1561 agccaagctg atctcctttg ccccggagat caccatggac gactttctct atctctacga
     1621 tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa
     1681 gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc
     1741 ggcaggtctg atcaagacga tctacccgag taataatctc caggagatca aataccttcc
     1801 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
     1861 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
     1921 taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa
     1981 ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga
     2041 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     2101 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     2161 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     2221 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     2281 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     2341 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     2401 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     2461 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt
     2521 tttacaacaa taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc
     2581 gaagaatgtc aggtaagcca tcttcctgct tatttttata atgaacatag aaataggaag
     2641 ttgtgcagag aaacgctgtc aggaggtgcc aagcttgcat gcctgcaggt agcagaaggc
     2701 atgttgttgt gactccgagg ggttgcctca aactctatct tataaccggc gtggaggcat
     2761 ggaggcaggg gtattttggt cattttaata gatagtggaa aatgacgtgg aatttactta
     2821 aagacgaagt ctttgcgaca agggggggcc cacgccgaat ttaatattac cggcgtggcc
     2881 cccccttatc gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aatttcccgc
     2941 ccactagggt taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg
     3001 agcgtatatt gtatcaggta tttccgttgg atacgaatta ttcgtacgac cctccgcttg
     3061 agacgaagct ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     3121 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     3181 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3241 agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag
     3301 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3361 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3421 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3481 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3541 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3601 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3661 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3721 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3781 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3841 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3901 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3961 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4021 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4081 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4141 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct
     4201 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4261 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4321 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat ccgatgagta aataaacaaa
     4381 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4441 gacgaccatc ggaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4501 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4561 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4621 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4681 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4741 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4801 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4861 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4921 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4981 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5041 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5101 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5161 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5221 accgagctgc tatctgaata gatcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5281 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5341 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5401 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     5461 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     5521 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     5581 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     5641 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     5701 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     5761 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     5821 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     5881 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     5941 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     6001 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     6061 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     6121 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     6181 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     6241 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     6301 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     6361 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     6421 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     6481 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     6541 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata
     6601 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     6661 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     6721 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     6781 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     6841 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     6901 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     6961 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     7021 aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg
     7081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7141 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     7201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7261 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8101 gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga
     8161 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     8221 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     8281 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     8341 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     8401 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     8461 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg
     8521 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     8581 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     8641 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     8701 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     8761 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     8821 aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     8881 aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata
     8941 tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca
     9001 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     9061 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     9121 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     9181 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     9241 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     9301 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     9361 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac aagcttcgtc
     9421 tcagtca
//