LOCUS       hCD12_plasmid_04        8737 bp    DNA     circular SYN 16-JUL-2024
DEFINITION  hCD12_plasmid_04.
ACCESSION   hCD12_plasmid_04
VERSION     hCD12_plasmid_04
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      7253..7681
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1016..1207)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3611..4711
                     /note="pLannotate"
                     /label="MBP"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2016..3098)
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(7801..8616)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3099..3176)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4715..7015
                     /note="pLannotate"
                     /label="HDAC6 (fragment)"
                     /database="swissprot"
                     /identity="98.4"
                     /match_length="63.1"
                     /fragment="True"
                     /other="CDS"
     terminator      7169..7216
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    3504..3528
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     RBS             3543..3565
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        3485..3503
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3584..3601
                     /note="pLannotate"
                     /label="6xHis"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(775..914)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(1676..1967)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3184..3463)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.5"
                     /fragment="True"
                     /other="CDS"
     promoter        7735..7792
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="56.3"
                     /fragment="True"
                     /other="promoter"
     CDS             1224..1604
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(1982..2003)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             7112..7161
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             7085..7102
                     /note="pLannotate"
                     /label="9xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat accctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     3541 attttgttta actttaagaa ggagatatac catgggttct tctcaccatc accatcacca
     3601 tggttcttct atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg
     3661 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
     3721 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg
     3781 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt
     3841 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
     3901 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat
     3961 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
     4021 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt
     4081 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
     4141 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
     4201 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
     4261 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga
     4321 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa
     4381 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
     4441 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga
     4501 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg
     4561 tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat
     4621 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
     4681 tgtcgatgaa gccctgaaag acgcgcagac taatgggatc gaggaaaacc tgtacttcca
     4741 atccaatgca agtagtggcg ctggcactgg cttggtgttg gatgagcagt taaatgaatt
     4801 ccattgcctc tgggatgaca gcttcccgga aggccctgag cggctccatg ccatcaagga
     4861 gcaactgatc caggagggcc tcctagatcg ctgcgtgtcc tttcaggccc ggtttgctga
     4921 aaaggaagag ctgatgttgg ttcacagcct agaatatatt gatctgatgg aaacaaccca
     4981 gtacatgaat gagggagaac tccgtgtcct agcagacacc tacgactcag tttatctgca
     5041 tccgaactca tactcctgtg cctgcctggc ctcaggctct gtcctcaggc tggtggatgc
     5101 ggtcctgggg gctgagatcc ggaatggcat ggccatcatt aggcctcctg gacatcacgc
     5161 ccagcacagt cttatggatg gctattgcat gttcaaccac gtggctgtgg cagcccgcta
     5221 tgctcaacag aaacaccgca tccggagggt ccttatcgta gattgggatg tgcaccacgg
     5281 tcaaggaaca cagttcacct tcgaccagga ccccagtgtc ctctatttct ccatccaccg
     5341 ctacgagcag ggtaggttct ggccccacct gaaggcctct aactggtcca ccacaggttt
     5401 cggccaaggc caaggatata ccatcaatgt gccttggaac caggtgggga tgcgggatgc
     5461 tgactacatt gctgctttcc tgcacgtcct gctgccagtc gccctcgagt tccagcctca
     5521 gctggtcctg gtggctgctg gatttgatgc cctgcaaggg gaccccaagg gtgagatggc
     5581 cgccactccg gcagggttcg cccagctaac ccacctgctc atgggtctgg caggaggcaa
     5641 gctgatcctg tctctggagg gtggctacaa cctccgcgcc ctggctgaag gcgtcagtgc
     5701 ttcgctccac acccttctgg gagacccttg ccccatgctg gagtcacctg gtgccccctg
     5761 ccggagtgcc caggcttcag tttcctgtgc tctggaagcc cttgagccct tctgggaggt
     5821 tcttgtgaga tcaactgaga ccgtggagag ggacaacatg gaggaggaca atgtagagga
     5881 gagcgaggag gaaggaccct gggagccccc tgtgctccca atcctgacat ggccagtgct
     5941 acagtctcgc acagggctgg tctatgacca aaatatgatg aatcactgca acttgtggga
     6001 cagccaccac cctgaggtac cccagcgcat cttgcggatc atgtgccgtc tggaggagct
     6061 gggccttgcc gggcgctgcc tcaccctgac accgcgccct gccacagagg ctgagctgct
     6121 cacctgtcac agtgctgagt acgtgggtca tctccgggcc acagagaaaa tgaaaacccg
     6181 ggagctgcac cgtgagagtt ccaactttga ctccatctat atctgcccca gtaccttcgc
     6241 ctgtgcacag cttgccactg gcgctgcctg ccgcctggtg gaggctgtgc tctcaggaga
     6301 ggttctgaat ggtgctgctg tggtgcgtcc cccaggacac cacgcagagc aggatgcagc
     6361 ttgcggtttt tgctttttca actctgtggc tgtggctgct cgccatgccc agactatcag
     6421 tgggcatgcc ctacggatcc tgattgtgga ttgggatgtc caccacggta atggaactca
     6481 gcacatgttt gaggatgacc ccagtgtgct atatgtgtcc ctgcaccgct atgatcatgg
     6541 caccttcttc cccatggggg atgagggtgc cagcagccag atcggccggg ctgcgggcac
     6601 aggcttcacc gtcaacgtgg catggaacgg gccccgcatg ggtgatgctg actacctagc
     6661 tgcctggcat cgcctggtgc ttcccattgc ctacgagttt aacccagaac tggtgctggt
     6721 ctcagctggc tttgatgctg cacgggggga tccgatgggg ggctgccagg tgtcacctga
     6781 gggttatgcc cacctcaccc acctgctgat gggccttgcc agtggccgca ttatccttat
     6841 cctagagggt ggctataacc tgacatccat ctcagagtcc atggctgcct gcactcgctc
     6901 cctccttgga gacccaccac ccctgctgac cctgccacgg cccccactat caggggccct
     6961 ggcctcaatc actgagacca tccaagtcca tcgcagatac tggcgcagct tacggtaata
     7021 acattggaag tggataacgg atccgaattc gagcgccgtc gacaagcttg cggccgcact
     7081 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     7141 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     7201 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     7261 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7321 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7381 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7441 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7501 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7561 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7621 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7681 tttaacaaac tagtaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     7741 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     7801 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     7861 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     7921 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     7981 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     8041 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     8101 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     8161 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     8221 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaatg ttttcacctg aatcaggata
     8281 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     8341 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     8401 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     8461 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     8521 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     8581 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     8641 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     8701 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
