LOCUS       pcDNA4TO_T21R-TM        6078 bp    DNA     circular     09-Aug-2024
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     source          1..6078
                     /label="source"
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        236..812
                     /label="promoter "CMV_immearly_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     315..602
                     /label="misc_feature "CAG_enhancer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     769..789
                     /label="misc_feature "CMV_fwd_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        770..893
                     /label="promoter "CMV_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        782..955
                     /label="promoter "CMV2_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     820..859
                     /label="misc_feature "tetO" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     869..893
                     /label="misc_feature "LNCX_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     2089..2106
                     /label="misc_feature "BGH_rev_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     terminator      2092..2319
                     /label="terminator "bGH_PA_terminator" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffafaf"
     rep_origin      2382..2688
                     /label="rep_origin "f1_origin" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     misc_feature     2802..2822
                     /label="misc_feature "pBABE_3_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     misc_feature     2808..3023
                     /label="misc_feature "SV40_enhancer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        2820..3088
                     /label="promoter "SV40_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     rep_origin      2987..3064
                     /label="rep_origin "SV40_origin" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffff00"
     misc_feature     3049..3068
                     /label="misc_feature "SV40pro_F_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        3182..3249
                     /label="promoter "EM7_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     3250..3624
                     /label="misc_feature "sh_ble" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     gene            3250..3621
                     /label="gene "bleo" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffcc99"
     terminator      3757..3876
                     /label="terminator "SV40_PA_terminator" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffafaf"
     misc_feature     3845..3864
                     /label="misc_feature "EBV_rev_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        3920..3938
                     /label="promoter "M13_reverse_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature     3937..3959
                     /label="misc_feature "M13_pUC_rev_primer" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#c8c8ff"
     promoter        3973..4002
                     /label="promoter "lac_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     gene            5082..5942
                     /label="gene "Ampicillin" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ffcc99"
     CDS             complement(5082..5942)
                     /label="CDS "ORF frame 2" "
                     /ApEinfo_fwdcolor="#ffc800"
     promoter        5984..6012
                     /label="promoter "AmpR_promoter" "
                     /dnadynamo_directionless_feature="true"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             1347..2057
                     /label="mCherry"
                     /ApEinfo_fwdcolor="#ff3333"
     misc_feature     1284..1346
                     /label="T2A"
                     /ApEinfo_fwdcolor="#cc00cc"
     misc_feature     1002..1007
                     /label="Kozac"
                     /ApEinfo_fwdcolor="#00cccc"
     misc_feature     1053..1172
                     /label="T21 RING (UniProt)"
                     /ApEinfo_fwdcolor="#cc0000"
     CDS             1008..1262
                     /label="TRIM21 RING 1-85"
                     /ApEinfo_fwdcolor="#9933ff"
     misc_feature     1263..1277
                     /label="Linker"
                     /ApEinfo_fwdcolor="#ffff00"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgccaccatg gcttcagcag 
     1021 cacgcttgac aatgatgtgg gaggaggtca catgccctat ctgcctggac cccttcgtgg 
     1081 agcctgtgag catcgagtgt ggccacagct tctgccagga atgcatctct caggttggga 
     1141 aaggtggggg cagcgtctgt cctgtgtgcc ggcagcgctt tctgctcaag aatctccggc 
     1201 ccaatcgaca gctagccaac atggtgaaca accttaaaga aatcagccag gaggccagag 
     1261 agggtggagg cggttcagaa ttcggctccg gcgagggcag gggaagtctt ctaacatgcg 
     1321 gggacgtgga ggaaaatccc ggcccaatgg tgagcaaggg cgaggaggat aacatggcca 
     1381 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 
     1441 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 
     1501 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 
     1561 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 
     1621 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 
     1681 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 
     1741 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 
     1801 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 
     1861 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 
     1921 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 
     1981 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 
     2041 acgagctgta caagtgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc 
     2101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     2161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     2221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     2281 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     2341 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     2581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     2701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     2761 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     2821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     2881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     2941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     3061 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3121 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca 
     3181 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg 
     3241 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc 
     3301 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac 
     3361 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg 
     3421 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag 
     3481 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc 
     3541 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac 
     3601 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc 
     3661 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     3721 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 
     3781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     3841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     3901 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     3961 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     4021 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     4081 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     4141 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     4201 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     4261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     4321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     4381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     4441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     4501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     4561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     4621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     4681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     4741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     4801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     4921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     4981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     5041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     5101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     5161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     5221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     5281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     5341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     5401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     5461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     5521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     5581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     5641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     5701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     5761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     5821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     5881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     5941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     6001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     6061 aaagtgccac ctgacgtc
//