LOCUS       pEND-350_Cacnes_ori     6029 bp ds-DNA     circular     02-AUG-2024
DEFINITION  .
COMMENT     !
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label="XbaI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    117..122
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    123..152
                     /label="VL2017mar22_14"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    153..158
                     /label="SacI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    159..170
                     /label="REspacer2017mar27_8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    171..176
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    177..206
                     /label="VL2017mar22_0"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    207..212
                     /label="NcoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    213..302
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    303..502
                     /label="2017mar28_200bpSpacer1* (C->G change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    425..425
                     /label="C->G change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    503..508
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    509..520
                     /label="REspacer2017mar27_7"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    521..526
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    527..556
                     /label="VL2017mar22_10"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    557..564
                     /label="SbfI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    565..625
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    626..825
                     /label="2018mar28_200bpSpacer2.5* (A->T change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    687..687
                     /label="A->T change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    826..831
                     /label="SalI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    832..843
                     /label="REspacer2017mar27_5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    844..849
                     /label="AatII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    850..879
                     /label="VL2017mar22_11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    880..887
                     /label="NotI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    888..944
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    945..989
                     /label="VL2017mar15_8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    990..995
                     /label="NheI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    996..1007
                     /label="REspacer2017mar27_15"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1008..1014
                     /label="BbvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1015..1022
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1075..2813
                     /label="p545 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1094..1099
                     /label="former AGCAGY"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1346..2257
                     /label="Rep Prot1 - REs removed"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1346..2257
                     /label="Translation 1346-2257"
                     /translation="MDSFETLFPESWLPRKPLASAEKSGAYRHVTRQRALELPYIEANPLVMQSLVITDRDASDADWAADLAGLPSPSYVSMNRVTTTGHIVYALKNPVCLTDAARRRPINLLARVEQGLCDVLGGDASYGHRITKNPLSTAHATLWGPADALYELRALAHTLDEIHALPEAGNPRRNVTRSTVGRNVTLFDTTRMWAYRAVRHSWGGPVAEWEHTVFEHIHLLNETIIADEFATGPLGLNELKHLSRSISRWVWRNFTPETFRARQKAISLRGASKGGKEGGHKGGIASGASRRAHTRQQFLEGLS*"
     misc_feature    2254..2511
                     /label="Rep Prot2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2254..2511
                     /label="Translation 2254-2511"
                     /translation="MTTRERLPRNGYSIAAAAKKLGVSESTVKRWTSEPREEFVARVAARHARIRELRSEGQSMRAIAAEVGVSVGTVHYALNKNRTDA*"
     misc_feature    complement(2299..2304)
                     /label="former AGCAGY"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2412..2412
                     /label="C->G change to remove SacI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2915..2920
                     /label="BclI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2915..2920
                     /label="Blocked by dam/dcm methylation !!!"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(2939..3527)
                     /label="PBR322 ori with no Rop (low-copy version of pUC ori)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3380..3380
                     /label="A->G change to reduce copy number"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3604..3609
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3610..4470)
                     /label="AmpR (codon-optimized for C. acnes)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(3610..4470)
                     /label="Translation 4470-3610"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    complement(4471..4575)
                     /label="P(AmpR)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4576..4581
                     /label="SpeI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4675..5820)
                     /label="ermE (codon-optimized for C. acnes)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(4675..5820)
                     /label="Translation 5820-4675"
                     /translation="MSSSDEQPRPRRRNQDRQHPNQNRPVLGRTERDRNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPGEGLLTRELADRARQVTSYEIDPRLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETATMVTQLEFARKRTGDYGRWSRLTVMTWPLFEWEFVEKVDRRLFKPVPKVDSAIMRLRRRAEPLLEGAALERYESMVELCFTGVGGNIQASLLRKYPRRRVEAALDHAGVGGGAVVAYVRPEQWLRLFERLDQKNEPRGGQPQRGRRTGGQDRGDRRTGGQDRGDRRTGGQDRRDRQASGHGDRRSSGRNRDDGRTGEREQGDQGGRRGPSGGGRTGGRPGRRGGPGQR*"
     misc_feature    complement(5821..6029)
                     /label="P(roxP) + RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tctagaGGAA ATCAGAGCTT GAGGCTGCCG GAGCGACGGT AGACCCGTCC GGTTGGGTGG
       61 TGCCACTGCG TGCACTCAAG GTCGTTTTTG GGGTGTCAGA TGtGACCTCG AATGCGGAAT
      121 TCATAGGATA GATTCTGGAA ACTTTACCGT CCGAGCTCAG GCTTACCTTA CTCGAGCAAT
      181 AAACAGTTGA TAGGGCTTCT CCGTTACCAT GGTTCAGCCA AAAAACTTAA GACCGCCGGT
      241 CTTGTCCACT ACCTTGCAGT AATGCGGTGG ACAGGATCGG CGGTTTTCTT TTCTCTTCTC
      301 AATTCTTCTG ACCTGTAACG AATAATAGAT AGTAAAGTAG TCTCCGATTG AGTTTTCTCT
      361 GCCGAGTCCC ACCCAGTTCT GTGATTTCAG TAAGTTGGTA ATTGATACAC TGTTGCGAGA
      421 ACTGgTGCCT GGTAGTAGAT AGGTTGTTAT TGAGTAAGAA GGTAAAGTGA ACGAAATCCC
      481 TGAAACTGAG ACTGTAGAAA ATAAGCTTGT CCAGACTATT GGATCCAAGA GATTTCTACA
      541 CGATTGAGCA CTGTCTCCTG CAGGCTCGGT ACCAAATTCC AGAAAAGAGG CCTCCCGAAA
      601 GGGGGGCCTT TTTTCGTTTT GGTCCTAATA GATAAAGGAT AGGTCTGGTA GTGTTGTTCG
      661 TTCTCGCAGG TAAATCAATA ATACTCtGCA GTTCCGTAGA CTTTTCAGTG GGACAGGGTA
      721 GCGATAACAG ATAGATTGTA ATAAGACACA GTAGGTGCTC GTAGTTGCGT GAAGAGAACC
      781 GCTCAGGAAA TCCAGTCAGA AGTATTGGTA ATCGTTGAAA ACTCAGTCGA CGCACTTACT
      841 GAAGACGTCC TATTACACTC GTCGTTGGAA ACTGAAGATG CGGCCGCgga aacacagAAA
      901 AAAGCCCGCA CCTGACAGTG CGGGCTTTTT TTTTcgacca aaggTAGCGA ACGACGAGTC
      961 ACTGTTGAGG ATAAATACTT TCTCTACTAG CTAGCTGTTA CACAGGTCCT CAGCGGCGCG
     1021 CCcgCCCGGT CATGACGCTG AGTTAGTGGC GCAGCTGCGC TCTGAGAACG AGTTTCGACC
     1081 CTGACAGCCG GCGAGCtGTT CAGGCGAAGA TCGCACAGCT GCGCGAGGAA CTAGCCGCAA
     1141 TGCCCGAACA CGCCCCAGCC ATCCCTTGGA GCAGGTGGCA GCGTCAGGGG AGTCGGGGGA
     1201 TGTTTGGCAG GGGATGTGGA AAGAGAGTTC GCTTTGCTCA CATGGCTCAA CCGGGTAACT
     1261 AACTGATATG GGcTCTTCGT CGCCCACTTT GAACACGCCG AGGAATGGAC CACGCTGAAC
     1321 GTGACTCGCA TGCTTCACTG CATGTATGGA TTCGTTCGAG ACtTTGTTCC CTGAGAGCTG
     1381 GCTGCCACGC AAGCCGCTGG CGTCAGCCGA GAAGTCTGGG GCGTACCGGC ACGTGACTCG
     1441 GCAGAGGGCG CTGGAGCTGC CTTACATCGA AGCGAACCCG TTGGTCATGC AGTCCTTGGT
     1501 CATCACCGAT CGAGATGCTT CGGATGCTGA CTGGGCCGCA GACCTCGCTG GGCTGCCTTC
     1561 ACCGTCCTAC GTGTCCATGA ACCGTGTCAC GACCACCGGA CACATCGTCT ATGCCTTGAA
     1621 GAACCCTGTG TGTCTGACCG ATGCCGCGCG GCGACGGCCT ATCAACCTGC TCGCCCGCGT
     1681 CGAGCAGGGC CTATGCGACG TTCTCGGCGG CGATGCATCC TACGGGCACC GGATCACAAA
     1741 GAACCCGCTC AGCACCGCCC ATGCGACCCT CTGGGGCCCC GCAGACGCGC TCTACGAGCT
     1801 GCGCGCCCTC GCACACACCC TCGACGAGAT CCACGCACTG CCGGAGGCAG GGAACCCGCG
     1861 TCGCAACGTC ACCCGATCAA CGGTCGGCCG CAACGTCACC CTGTTCGACA CCACCCGCAT
     1921 GTGGGCATAC CGGGCCGTCC GGCACTCCTG GGGCGGCCCG GTCGCCGAAT GGGAGCACAC
     1981 CGTATTCGAG CACATCCACC TACTGAACGA aACGATCATC GCCGACGAgT TCGCCACAGG
     2041 CCCCCTCGGC TTGAACGAAC TTAAGCACTT ATCTCGATCC ATTTCCCGAT GGGTCTGGCG
     2101 CAACTTCACC CCCGAAACCT TCCGCGCACG CCAGAAAGCG ATCAGCCTCC GTGGAGCATC
     2161 CAAAGGCGGC AAAGAAGGCG GCCACAAAGG CGGCATTGCC AGTGGCGCAT CACGGCGtGC
     2221 CCATACCCGT CAACAGTTCT TGGAGGGTCT gTCATGACCA CACGTGAACG cCTCCCCCGC
     2281 AACGGCTACA GCATCGCCGC cGCTGCGAAA AAGCTCGGTG TCTCCGAGTC CACCGTCAAG
     2341 CGGTGGACTT CCGAGCCACG CGAGGAGTTC GTGGCCCGCG TTGCCGCACG CCACGCGCGG
     2401 ATTCGTGAGC TgCGCTCGGA GGGTCAGAGC ATGCGTGCGA TTGCTGCCGA GGTCGGGGTT
     2461 TCCGTGGGCA CCGTGCACTA CGCGCTGAAC AAGAATCGAA CTGACGCATG ACCGTAACGC
     2521 CGCACGATGA GCATTTTCTT GATCGTGCAC CGCTTGGCAC TACGTTCGCG TGCGGTTGCA
     2581 CAGTGCGCGC CACGTTCTTA TCCTGCGGCC ATTGTGGCTA CAGCCAATGG GGGGCATCAG
     2641 CAACGGACGT TGAACCCGGT GGGCAAGTGT TACTCAGGGG GACATGCCCA GTCTGCGGCG
     2701 CTCGGATTGA CGGTATGGCA GTCGTGCATG CGGCCCCACC GTCAAACTCA TTCAGGTATC
     2761 AGTGAGAACC CTCATGGCAC CCCCTCGTGA CACGTTCTCG TTGCGATCAG CTGCGGCCAA
     2821 GCTGATCTTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG CATGATCGTG CTCCTGTCGT
     2881 TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACtgatca GGCCGCGTTG CTGGCGTTTT
     2941 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
     3001 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
     3061 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
     3121 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     3181 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     3241 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     3301 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     3361 ACTACGGCTA CACTAGAAGg ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     3421 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     3481 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     3541 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     3601 TGAGGCGCCT CACCAaTGCT TGATgAGGGA GGCACCGATC TCGGCGATCT GACGGTTGCG
     3661 CTCATCCATC GTGGCCTGGG AGCCGGTCGT GTAGATCACC ACGATGCGGC TGGGCTTACC
     3721 GTCgGGACCg AGAGCCGCGA TGATGCCACG CGAGCCGCGC TCACCAGCAC CCGACTTGTC
     3781 CGCGATGAAC CAACCGGCCG GGAGCGCGCT GCGCAACAGC GGGCCAGCGA CCTTGTCGGC
     3841 CTCCATCCAG TCGATCAGTT GCTGACGGGA GGCCAGAGTG AGGAGTTCAC CGGTCAGGAG
     3901 TTTACGGAGG GTGGTAGCCA TAGCCGCGGG CATAGTCGTG TCGCGCTCGT CgTTcGGgAT
     3961 AGCCTCATTA AGTTCGGGTT CCCAACGGTC CAGACGGGTC ACGTGGTCGC CCATGTTGTG
     4021 GAGAAAAGCC GTCAGCTCTT TCGGGCCACC GATGGTCGTC AACAGCAGAT TGGCGGCAGT
     4081 GTTGTCGGAC ATAGTGATGG CCGCGGAGCA CAGCTCGCGC ACGGTCATCC CATCGGTCAG
     4141 ATGTTTCTCC GTGACCGGGG AGTATTCGAC CAGGTCGTTC TGGGAGTAAT GAATGCGACG
     4201 GCCCAGTTGC TCCTGCCCAG CATCCACCCG GCTAAGCACA GCGCCGCAGA GGAGGACCTT
     4261 GAACGTGGAC ATCATGGGGA AACGCTCCTC AGGGCGGAAA GACTCCAGAA TCTTACCGGA
     4321 GTTCAGGTCC AACTCGATAT AACCGACGCG CGCCCCCAGC TGATCCTCGG CGTCCTTGAC
     4381 CTTGACCAGG GTtTCCGGAT GGGCAAAAAC CGGCAAGCAA AAGGCCGCAA AAAATGGGAT
     4441 AAGGGCAACG CGGAAGTGCT GGATcGACAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     4501 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     4561 AATAGGGGTT CCGCGactag tTCCCCGAAA AGTGCCACCT GACGTTAAGA AACCATTATT
     4621 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT aCAATCAGCG
     4681 CTGACCCGGA CCGCCACGAC GGCCTGGACG ACCGCCGGTA CGACCACCAC CCGAAGGGCC
     4741 ACGACGACCA CCCTGGTCGC CCTGCTCGCG CTCCCCGGTA CGACCATCGT CGCGATTACG
     4801 GCCAGAGGAC CGACGGTCAC CGTGGCCGGA GGCCTGACGG TCGCGACGGT CCTGACCACC
     4861 CGTCCGACGG TCGCCACGAT CCTGGCCACC CGTACGGCGG TCACCACGAT CCTGACCACC
     4921 CGTACGACGG CCGCGCTGCG GTTGGCCACC GCGCGGCTCG TTCTTCTGGT CAAGGCGCTC
     4981 GAAGAGCCGC AGCCATTGCT CCGGCCGGAC GTAGGCCACG ACAGCACCAC CACCCACGCC
     5041 GGCATGGTCC AGCGCAGCCT CCACGCGaCG aCGaGGGTAC TTGCGCAGGA GGGAGGCTTG
     5101 AATGTTGCCG CCGACACCAG TAAAACACAA CTCCACCATG CTCTCGTAGC GCTCAAGGGC
     5161 AGCACCCTCC AACAGCGGTT CAGCGCGGCG ACGCAGACGC ATGATGGCGG AATCGACCTT
     5221 CGGGACCGGC TTGAACAAGC GGCGATCGAC CTTCTCGACG AACTCCCACT CGAAGAGCGG
     5281 CCAAGTCATG ACCGTGAGGC GGGACCAGCG GCCATAATCA CCGGTGCGCT TACGGGCAAA
     5341 TTCGAGTTGG GTAACCATCG TGGCGGTtTC GATCGTCGGA GCCTCCAGGC ACCAATCGAC
     5401 GATCGCAGAC GTGATGCCGT ACGGGATCGC GCCGACGAAG GCAAACGGCT CGGGAGGGGG
     5461 CTCGGCGGTC AGGAAGTCCG CGTTGACGAC CTCGATATTC GGGTGGCCGG ACAGCTTCTC
     5521 ACGCAGGGAC TTCGCCAAGC GCGGGTCGAT CTCGTAGCTC GTCACCTGGC GGGCCCGATC
     5581 GGCAAGCTCA CGGGTCAGCA GACCCTCGCC TGGGCCAGCC TCCAGGACCG GAAGGTCGGG
     5641 GCGCAGTTCG GCCGTTTCCG CGATGCGGGC GATCGTCTTA CGGTCACGAA GGAAATTCTG
     5701 ACCgAAtTGa CGGCGGTTGC GGTCCCGCTC AGTACGGCCC AGGACCGGGC GGTTCTGGTT
     5761 CGGGTGCTGG CGGTCCTGGT TGCGGCGACG CGGGCGCGGT TGCTCGTCGG ACGACGACAT
     5821 GACGATCTCC CGATGTGTCG TACGTATGGG AATTGACGAA AATCATATAG ATTTTCAGTT
     5881 GAAGTGTAGC ACTGCATAGT GTCATCGAAG TGTCGGCGGC AACGGTATGC CCGGTAGGGG
     5941 GTGGGTCTGC CTTTCGGTTT CGTGAACCTG ATGCGGCGAC GCGCATGTAG CCGATAACCC
     6001 CTAGACTCGC CCGTGCAGAT CGTCGGTGT
//