LOCUS       pEND-418_Cacnes_LysA    4523 bp ds-DNA     circular     02-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    983..988
                     /label="XbaI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     terminator      989..1024
                     /label="ECK120030221"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="ECK120030221"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNR159"
                     /Transferred_Similarity="100.00%"
     attP1           1065..1122
                     /label="BxbIP-GT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /created_by="User"
                     /modified_by="User"
     misc_feature    1163..1168
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1169..1198
                     /label="VL2017mar22_22"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1199..1213
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1199..1728
                     /label="MG-230"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1214..1713
                     /label="HR LysALysinePPA_RS06345 PPA1259 AAT83006 Q6A8A9"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            1284..1713
                     /label="PPA_RS06340"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /old_locus_tag="PPA1258"
     CDS             1284..1713
                     /label="PPA_RS06340"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /old_locus_tag="PPA1258"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AAsequence:RefSeq:WP_002551109.1"
                     /product="homoserine dehydrogenase"
                     /protein_id="WP_002517921.1"
                     /transl_table=11
                     /translation="MTNTTDSASAQPIRVALLGCGVVGSQVARMIESRTEDIAARVGRQVELAGIAVAHPDRPRQGLDSRLFTDDPTVLVNRDDVDIVIELIGGIDPARQLLTAALGKGHSVVTANKALLAAHGTELHAVAESNGVDLYYEAAVAGA"
     misc_feature    1714..1728
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1729..1748
                     /label="Overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1752..1757
                     /label="BclI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1752..1757
                     /label="Blocked by dam/dcm methylation !!!"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(1776..2364)
                     /label="PBR322 ori with no Rop (low-copy version of pUC ori)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2217..2217
                     /label="A->G change to reduce copy number"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2441..2446
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2447..3307)
                     /label="AmpR (codon-optimized for C. acnes)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(2447..3307)
                     /label="Translation 3307-2447"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    complement(3308..3412)
                     /label="P(AmpR)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3413..3418
                     /label="SpeI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3439..3458
                     /label="Overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3459..3473
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3459..3988
                     /label="MG-229"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            complement(3474..3627)
                     /label="PPA_RS06355"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /old_locus_tag="PPA1260"
     CDS             complement(3474..3627)
                     /label="PPA_RS06355"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /old_locus_tag="PPA1260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AAsequence:RefSeq:WP_002545928.1"
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="WP_002524923.1"
                     /transl_table=11
                     /translation="MLHVFIQPSSNYITGDLRYYLWWMSSGQPDSSVLPEYPLPVVWFMRTLYWA"
     misc_feature    3474..3973
                     /label="HL LysALysinePPA_RS06345 PPA1259 AAT83006 Q6A8A9"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(3781..3852)
                     /label="PPA_RS06350"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /old_locus_tag="PPA2429"
     tRNA            complement(3781..3852)
                     /label="PPA_RS06350"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Derived by automated computational analysis usinggene prediction method: tRNAscan-SE."
                     /old_locus_tag="PPA2429"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /anticodon="(pos:1364645..1364647,aa:Arg,seq:ccg)"
     misc_feature    3974..3988
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3989..4018
                     /label="VL2017mar22_22"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4019..4024
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     attB1           4062..4110
                     /label="BxbIB-GT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /created_by="User"
     misc_feature    4151..4359
                     /label="P(roxP) + RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4360..982
                     /label="ermE (codon-optimized for C. acnes)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4360..982
                     /label="Translation 4360-982"
                     /translation="MSSSDEQPRPRRRNQDRQHPNQNRPVLGRTERDRNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPGEGLLTRELADRARQVTSYEIDPRLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETATMVTQLEFARKRTGDYGRWSRLTVMTWPLFEWEFVEKVDRRLFKPVPKVDSAIMRLRRRAEPLLEGAALERYESMVELCFTGVGGNIQASLLRKYPRRRVEAALDHAGVGGGAVVAYVRPEQWLRLFERLDQKNEPRGGQPQRGRRTGGQDRGDRRTGGQDRGDRRTGGQDRRDRQASGHGDRRSSGRNRDDGRTGEREQGDQGGRRGPSGGGRTGGRPGRRGGPGQR*"
ORIGIN
        1 AACGGCCGAA CTGCGCCCCG ACCTTCCGGT CCTGGAGGCT GGCCCAGGCG AGGGTCTGCT
       61 GACCCGTGAG CTTGCCGATC GGGCCCGCCA GGTGACGAGC TACGAGATCG ACCCGCGCTT
      121 GGCGAAGTCC CTGCGTGAGA AGCTGTCCGG CCACCCGAAT ATCGAGGTCG TCAACGCGGA
      181 CTTCCTGACC GCCGAGCCCC CTCCCGAGCC GTTTGCCTTC GTCGGCGCGA TCCCGTACGG
      241 CATCACGTCT GCGATCGTCG ATTGGTGCCT GGAGGCTCCG ACGATCGAaA CCGCCACGAT
      301 GGTTACCCAA CTCGAATTTG CCCGTAAGCG CACCGGTGAT TATGGCCGCT GGTCCCGCCT
      361 CACGGTCATG ACTTGGCCGC TCTTCGAGTG GGAGTTCGTC GAGAAGGTCG ATCGCCGCTT
      421 GTTCAAGCCG GTCCCGAAGG TCGATTCCGC CATCATGCGT CTGCGTCGCC GCGCTGAACC
      481 GCTGTTGGAG GGTGCTGCCC TTGAGCGCTA CGAGAGCATG GTGGAGTTGT GTTTTACTGG
      541 TGTCGGCGGC AACATTCAAG CCTCCCTCCT GCGCAAGTAC CCtCGtCGtC GCGTGGAGGC
      601 TGCGCTGGAC CATGCCGGCG TGGGTGGTGG TGCTGTCGTG GCCTACGTCC GGCCGGAGCA
      661 ATGGCTGCGG CTCTTCGAGC GCCTTGACCA GAAGAACGAG CCGCGCGGTG GCCAACCGCA
      721 GCGCGGCCGT CGTACGGGTG GTCAGGATCG TGGTGACCGC CGTACGGGTG GCCAGGATCG
      781 TGGCGACCGT CGGACGGGTG GTCAGGACCG TCGCGACCGT CAGGCCTCCG GCCACGGTGA
      841 CCGTCGGTCC TCTGGCCGTA ATCGCGACGA TGGTCGTACC GGGGAGCGCG AGCAGGGCGA
      901 CCAGGGTGGT CGTCGTGGCC CTTCGGGTGG TGGTCGTACC GGCGGTCGTC CAGGCCGTCG
      961 TGGCGGTCCG GGTCAGCGCT GAtctagacc cgcacttaac ccgcttcggc gggtttttgt
     1021 tttttggggt gcaggtaggc catattgtct tcggggcccc actcgtcgtg gtttgtctgg
     1081 tcaaccaccg cggtctcagt ggtgtacggt acaaaccccg acgaggcgat aagctcggca
     1141 aggctgatta cgcctgctcg tgGAATTCTA GAACTCTGTG AGGATAAAGT CTCCCTTACA
     1201 GCCTGCGGTC CGGcgcatcg aaaagtccta ttacgcacta tctgtgaact ttcatcagct
     1261 gacaacaacg aggtgaggaa gagatgacga ataccaccga ttccgcgtca gcccagccga
     1321 tacgcgtggc cttgctgggg tgcggggttg tggggtccca ggtggcccgg atgattgaat
     1381 cgaggacgga ggatatcgcc gctcgagtgg ggcggcaggt cgagctcgct ggtattgctg
     1441 tcgcccatcc tgatcgcccg cgccagggcc ttgactcgcg tctttttacc gatgatccca
     1501 cagtgctcgt taaccgggac gatgtggata tcgtcatcga gctcatcggt ggcatcgacc
     1561 ctgcccgtca gctcctgaca gctgccctcg gcaaggggca ctccgttgtg acggcgaata
     1621 aggccctgtt ggcagctcac gggactgagt tacacgctgt ggccgagagc aacggcgtcg
     1681 atctgtacta cgaagctgcg gtagccgggg caaTCGCTGG GACGCCCGCT AGGCTGGCGG
     1741 GGTTGCCTTA CtgatcaGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     1801 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     1861 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     1921 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     1981 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     2041 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     2101 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     2161 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGgACA
     2221 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     2281 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     2341 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2401 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GGCGCCTCAC CAaTGCTTGA
     2461 TgAGGGAGGC ACCGATCTCG GCGATCTGAC GGTTGCGCTC ATCCATCGTG GCCTGGGAGC
     2521 CGGTCGTGTA GATCACCACG ATGCGGCTGG GCTTACCGTC gGGACCgAGA GCCGCGATGA
     2581 TGCCACGCGA GCCGCGCTCA CCAGCACCCG ACTTGTCCGC GATGAACCAA CCGGCCGGGA
     2641 GCGCGCTGCG CAACAGCGGG CCAGCGACCT TGTCGGCCTC CATCCAGTCG ATCAGTTGCT
     2701 GACGGGAGGC CAGAGTGAGG AGTTCACCGG TCAGGAGTTT ACGGAGGGTG GTAGCCATAG
     2761 CCGCGGGCAT AGTCGTGTCG CGCTCGTCgT TcGGgATAGC CTCATTAAGT TCGGGTTCCC
     2821 AACGGTCCAG ACGGGTCACG TGGTCGCCCA TGTTGTGGAG AAAAGCCGTC AGCTCTTTCG
     2881 GGCCACCGAT GGTCGTCAAC AGCAGATTGG CGGCAGTGTT GTCGGACATA GTGATGGCCG
     2941 CGGAGCACAG CTCGCGCACG GTCATCCCAT CGGTCAGATG TTTCTCCGTG ACCGGGGAGT
     3001 ATTCGACCAG GTCGTTCTGG GAGTAATGAA TGCGACGGCC CAGTTGCTCC TGCCCAGCAT
     3061 CCACCCGGCT AAGCACAGCG CCGCAGAGGA GGACCTTGAA CGTGGACATC ATGGGGAAAC
     3121 GCTCCTCAGG GCGGAAAGAC TCCAGAATCT TACCGGAGTT CAGGTCCAAC TCGATATAAC
     3181 CGACGCGCGC CCCCAGCTGA TCCTCGGCGT CCTTGACCTT GACCAGGGTt TCCGGATGGG
     3241 CAAAAACCGG CAAGCAAAAG GCCGCAAAAA ATGGGATAAG GGCAACGCGG AAGTGCTGGA
     3301 TcGACATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
     3361 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGactagtTC
     3421 CCCGAAAAGT GCCACCTGAC GTTAAGAAAC CATTATTACA GCCTGCGGTC CGGcggccca
     3481 atagagggtt cgcatgaacc acacaacagg cagcgggtat tccggcagga cggacgaatc
     3541 tggttgtccg ctcgacatcc accacaggta gtaccggagg tcacccgtga tgtagttcga
     3601 tgacggctgg ataaagacgt gaagcatgac gagtcgaccc actgtccaca gcacgatggc
     3661 acctgccgca gaaaacagcg cagctcggga tccttggcga gcggttttgg cgtcacgaaa
     3721 tggcacgaac tgacacccta cctgataaat ccaggtacgc cgctaaggac atagctcgag
     3781 cgctcccgga gggattcgaa ccctcgctcc cgcctccgga gggcggtgct ctatcccctg
     3841 agctacggga gccgacaggc ccgaaggccc cgaccacctg tggagtttag cgcaccggtg
     3901 ggctgctcgc cattcaagat cgcgagcatg gacactctgc gatcgtgtcg gtggcctctg
     3961 gcaaggtgta accTCGCTGG GACGCCCGTA GAACTCTGTG AGGATAAAGT CTCCCTTAAA
     4021 GCTTgcgcca accatgcacc atagccagcc tgacagtagc ccggccggct tgtcgacgac
     4081 ggcggtctcc gtcgtcagga tcatccgggc gcagctgaga gcgacacagt cctgtacttg
     4141 ggatgctggc ACACCGACGA TCTGCACGGG CGAGTCTAGG GGTTATCGGC TACATGCGCG
     4201 TCGCCGCATC AGGTTCACGA AACCGAAAGG CAGACCCACC CCCTACCGGG CATACCGTTG
     4261 CCGCCGACAC TTCGATGACA CTATGCAGTG CTACACTTCA ACTGAAAATC TATATGATTT
     4321 TCGTCAATTC CCATACGTAC GACACATCGG GAGATCGTCA TGTCGTCGTC CGACGAGCAA
     4381 CCGCGCCCGC GTCGCCGCAA CCAGGACCGC CAGCACCCGA ACCAGAACCG CCCGGTCCTG
     4441 GGCCGTACTG AGCGGGACCG CAACCGCCGt CAaTTcGGTC AGAATTTCCT TCGTGACCGT
     4501 AAGACGATCG CCCGCATCGC GGA
//