LOCUS       pEMS2374        4380 bp    DNA     circular     12-SEP-2018
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12462655|
COMMENT     VNTDBDATE|-12462655|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2374|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMS2374" 0 4380 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT         "NotI" "SalI" "SmaI" "EcoRV" "HindIII" "ScaI" "XbaI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1555 1555 1 4380 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 891 0 1671 0 3394 0 
COMMENT            0 "") #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2135 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 442 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 408 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2132 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2142 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 3874 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 637 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 494 0 982 0 1257 0 1450 
COMMENT             0 0 "") 
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COMMENT             0 0 "") 
COMMENT        #17=(CRSite (CStringList) "cat" "cat" 1 0 51 38 0 154 0 183 0 230 0 240 
COMMENT             0 247 0 508 0 584 0 725 0 895 0 955 0 1585 0 1591 0 1653 0 2155 0 
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COMMENT             0 4208 0 4229 0 4241 0 4288 0 4325 0 4334 0 4339 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "gtg" "gtg" 1 0 72 13 0 22 0 118 0 128 0 177 0 
COMMENT             191 0 193 0 282 0 325 0 394 0 515 0 531 0 533 0 625 0 630 0 665 0 
COMMENT             761 0 861 0 871 0 929 0 1020 0 1025 0 1051 0 1096 0 1161 0 1191 0 
COMMENT             1193 0 1195 0 1462 0 1555 0 1734 0 1737 0 1795 0 1833 0 1835 0 1839 
COMMENT             0 1850 0 1958 0 2055 0 2175 0 2177 0 2231 0 2245 0 2256 0 2318 0 
COMMENT             2504 0 2618 0 2676 0 2742 0 2784 0 2812 0 2814 0 2953 0 2972 0 2975 
COMMENT             0 3097 0 3188 0 3337 0 3399 0 3442 0 3529 0 3645 0 3648 0 3728 0 
COMMENT             3789 0 3861 0 3868 0 3899 0 3975 0 4060 0 4109 0 4302 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "cac" "cac" 1 0 62 36 0 59 0 180 0 204 0 370 0 
COMMENT             551 0 577 0 706 0 715 0 785 0 804 0 842 0 847 0 855 0 1029 0 1102 0 
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COMMENT             3955 0 4055 0 4063 0 4094 0 4159 0 4286 0 4306 0 4364 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "taa" "taa" 1 0 50 72 0 143 0 214 0 340 0 349 
COMMENT             0 380 0 447 0 921 0 1079 0 1115 0 1242 0 1246 0 2151 0 2227 0 2234 
COMMENT             0 2250 0 2262 0 2272 0 2331 0 2458 0 2486 0 2537 0 2640 0 2858 0 
COMMENT             2884 0 2921 0 2981 0 3207 0 3259 0 3264 0 3278 0 3288 0 3303 0 3331 
COMMENT             0 3406 0 3496 0 3566 0 3589 0 3605 0 3776 0 3821 0 3847 0 3944 0 
COMMENT             3970 0 4050 0 4150 0 4265 0 4317 0 4342 0 4349 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "tta" "tta" 1 0 49 142 0 300 0 339 0 500 0 555 
COMMENT             0 570 0 619 0 920 0 1114 0 1125 0 1821 0 1824 0 2186 0 2271 0 2330 
COMMENT             0 2466 0 2704 0 2850 0 2895 0 2929 0 3037 0 3124 0 3206 0 3228 0 
COMMENT             3258 0 3263 0 3277 0 3320 0 3330 0 3426 0 3486 0 3543 0 3565 0 3604 
COMMENT             0 3709 0 3742 0 3792 0 3805 0 3829 0 3969 0 4023 0 4087 0 4199 0 
COMMENT             4211 0 4220 0 4256 0 4327 0 4330 0 4341 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "tga" "tga" 1 0 64 14 0 17 0 23 0 33 0 172 0 
COMMENT             194 0 395 0 430 0 456 0 534 0 603 0 626 0 631 0 666 0 750 0 884 0 
COMMENT             910 0 915 0 1196 0 1203 0 1209 0 1229 0 1457 0 1556 0 1570 0 1629 0 
COMMENT             1647 0 1669 0 1719 0 1761 0 1796 0 2178 0 2253 0 2257 0 2334 0 2399 
COMMENT             0 2505 0 2582 0 2870 0 2968 0 3028 0 3066 0 3160 0 3179 0 3223 0 
COMMENT             3270 0 3299 0 3314 0 3338 0 3387 0 3451 0 3714 0 3862 0 3869 0 3891 
COMMENT             0 4033 0 4071 0 4110 0 4170 0 4203 0 4231 0 4247 0 4310 0 4336 0 0 
COMMENT             "") 
COMMENT        #23=(CRSite (CStringList) "tca" "tca" 1 0 83 58 0 101 0 111 0 156 0 232 
COMMENT             0 250 0 369 0 462 0 714 0 724 0 800 0 808 0 894 0 985 0 1062 0 1101 
COMMENT             0 1121 0 1169 0 1489 0 1529 0 1538 0 1549 0 1595 0 1600 0 1674 0 
COMMENT             1768 0 1814 0 1878 0 1921 0 1983 0 2052 0 2072 0 2154 0 2160 0 2194 
COMMENT             0 2266 0 2286 0 2395 0 2439 0 2444 0 2448 0 2478 0 2591 0 2607 0 
COMMENT             2612 0 2753 0 2760 0 2776 0 2831 0 3146 0 3185 0 3201 0 3220 0 3231 
COMMENT             0 3244 0 3282 0 3334 0 3351 0 3372 0 3471 0 3489 0 3651 0 3675 0 
COMMENT             3679 0 3699 0 3769 0 3795 0 3799 0 3835 0 3876 0 3885 0 3933 0 3979 
COMMENT             0 3982 0 4014 0 4077 0 4093 0 4177 0 4193 0 4214 0 4228 0 4333 0 
COMMENT             4363 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "tag" "tag" 1 0 34 443 0 512 0 556 0 571 0 620 
COMMENT             0 638 0 644 0 757 0 832 0 1472 0 1641 0 1655 0 2163 0 2567 0 2756 0 
COMMENT             2768 0 2787 0 2930 0 2947 0 2996 0 3059 0 3092 0 3249 0 3379 0 3402 
COMMENT             0 3584 0 3593 0 3608 0 3743 0 3897 0 3959 0 4257 0 4273 0 4355 0 0 
COMMENT             "") 
COMMENT        #25=(CRSite (CStringList) "cta" "cta" 1 0 30 146 0 297 0 442 0 511 0 637 
COMMENT             0 756 0 1245 0 1497 0 1650 0 1913 0 2249 0 2261 0 2637 0 2861 0 
COMMENT             2956 0 2980 0 2984 0 2990 0 2995 0 3169 0 3248 0 3287 0 3346 0 3363 
COMMENT             0 3409 0 3562 0 3583 0 3634 0 4316 0 4346 0 0 "")) 
COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "bla(ApR)" 4 0 1 3320 4180 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3320..4180)") 
COMMENT        #27=(CFSignal (CObList) "rep(pMB1)" 33 0 1 2546 3160 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2546..3160)") 
COMMENT        #28=(CFSignal (CObList) "Ple391" 21 0 0 402 2140 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "402..2140")) (CObList) (CObList) (CObList) 
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COMMENT       (CTextView 0 
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COMMENT                          "DNA Plasmid 'pEMS2374'" 1) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 09/12/18 02:10PM" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/12/18 02:10PM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4380 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 7-SEP-2018" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 732-885-9188  FAX: 732-210-0262" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:gene@genscript.com\">gene@genscript.com</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genscript.com\">www.genscript.com</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                        "Start: 3320  End: 4180 (Complementary)" 
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COMMENT                                        "   complement(3320..4180)" 1))) 
COMMENT                                 "bla(ApR)"))) "CDS (1 total)") 
COMMENT                     #58=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
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COMMENT                                   #60=(CLinePar 
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COMMENT                                        "Start: 402   End: 2140" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   402..2140" 1))) "Ple391"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #63=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 2546  End: 3160 (Complementary)" 
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COMMENT                                        "   complement(2546..3160)" 1))) 
COMMENT                                 "rep(pMB1)"))) "Replication Origin (1 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 891 " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1671 " 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 3394 " 1))))) "AhdI: 3 sites") 6 5 
COMMENT                          "GACNNNNNGTC" "CTGNNNNNCAG") 
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COMMENT                          "GGCGCGCC" "CCGCGCGG") 
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COMMENT                          "CCTAGG" "GGATCC") 
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COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                          "GGCCGGCC" "CCGGCCGG") 
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COMMENT                                        " N4: 1450 " 1))))) "PstI: 4 sites") 5 1 
COMMENT                          "CTGCAG" "GACGTC") 
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COMMENT                           3 "AGTACT" "TCATGA") 
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FEATURES             Location/Qualifiers
     CDS             complement(3320..4180)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(2546..3160)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    402..2140
                     /vntifkey="21"
                     /label=Ple391
BASE COUNT     1081 a      1120 c      1198 g       981 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggccggcca aggcagcagg 
      421 acaggcagct gatgggcagg cctaggtaaa ggctctgact ctcaaggttg caaggttgcc 
      481 caagtttcct gcagtctctt tacctggcat ctaggtggca gggggcaggg gtgtgagaag 
      541 gcagaaaggg cactttagaa gcaaggaagt tagagccacc aagcatgggc tgggggctgg 
      601 gctgaccttt cctttccttt agctgtgacg tgagctctag aaatagaggc aggaaggaag 
      661 gagggtgaag ggcaggggcg ggaggcgggg caggggaaag ttgcccacag ttgtcacgag 
      721 gcttcatgtc tttctgccag acagcagatt gacagctaga gtgggcaggg ggagggctgg 
      781 gctccaccct ctccccctct cagcacttca ggggcaaaga aatgggagga gtaggacccg 
      841 acacgacacg ggaacacaag gtggaagggg gtggcccagg ctctgactct ctgtcattgg 
      901 aggaaatgct gagatgaagt taatccttgt ggcccagaga ggctggattc cagccatgca 
      961 gggccgactc cctgctctgc agagtcagtc ctctgcccct gctcttccct ctccctggag 
     1021 tgttgtgcca ccttctcccc ttgggaactg gtgttgctcc ttcaatgccc ggttcctgta 
     1081 aagtctcgct tttttgtgct tcacagcacc tgtttaagcc tcagttattt ccctgggggt 
     1141 cccctctctg gattgccagg gtgcgggatc aggggcctgg ttttctttct gtgtgtgact 
     1201 tctgaatctg acacagaatc tggcaaactg aggacagttg gtaactaatt gctgcagaag 
     1261 aaagaaaaga aagagaaaga aagaaaaaga aaggaaggaa gaaagaaaga aagaaaggaa 
     1321 agaaagaaag agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag 
     1381 aaagaaaaga aaagaaaaga aagaaaagga gagaggaaga aaaggggaga agaacgaggt 
     1441 tcctctgcag cccaaatgac agtgggcagg gtagcagcgg aacacccttc accactctat 
     1501 ctctttctcc ctctccccac tccctgcctc acacacctca gtttggagtc aagggtgagc 
     1561 tggaacccct gaggcctcct tttgcatttc catgtcaggt caactccaaa caggcttttt 
     1621 cctttggatg agtccagcaa tagaactgac tacatagatt ccaagacctg aagtcagaaa 
     1681 acggatgcgg acctcttcca acacctcctc tccttgcctg acttgctgga tgggtggtgc 
     1741 ctcccgcaac ccttggcttc tgagtcctca aaggagaaga ctggcgagca gacggtgagg 
     1801 acccagcctg caatcacagc ttattactct gggtgtgggt gggagcgcag tgggcggtcg 
     1861 agggttgcgt tcccgcctca aaacaaggga tggcggaatc ccccaacccc tcctacccgt 
     1921 tcaggccggg gatcgccgag gaggtacagc tcctttggtg gggggcgggg gcggggcctg 
     1981 tctcaggggc ggggaccggg gcacctccct cccgcgctcc ccgcgctcgg gcgccgcaga 
     2041 gctgtccagc ttcagtgccg aaccggcagc ctcacgcgcg caccgcgccg cctccgcccc 
     2101 gtccccgcgc tccctcctgc ccgcccgccc cgggcgcgcc aagcttggcg taatcatggt 
     2161 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     2221 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     2281 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     2341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     2401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     2461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     2521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     2581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     2641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     2701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     2761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     2821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     2881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     2941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     3001 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     3061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     3121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     3181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     3241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     3301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     3361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     3421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     3481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     3541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     3601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     3661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     3721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     3781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     3841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     3901 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     3961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     4021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     4081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     4141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     4201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     4261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 
     4321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
//
