LOCUS       pEMS2387        6337 bp    DNA     circular     11-SEP-2018
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12442594|
COMMENT     VNTDBDATE|-12461378|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2387|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                     "  Tm: 75.9 C  TaOpt: 53.7 C  GC: 42.8" 1) 
COMMENT                               #264=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #265=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #266=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #267=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #268=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #269=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #270=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #271=(COlyLinePar 
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COMMENT                                      (CParagraph 0 
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COMMENT                                        #272=(COligo "" "TCCTGTGGGACTCGCAAACA" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  TCCTGTGGGACTCGCAAACA" 1)) 
COMMENT                               #273=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #274=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 56.2 C  GC: 55.0" 1) 
COMMENT                               #275=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -149.3 kcal/mol  dS: -378.7 cal/mol  dG: -34.6 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #276=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 4.7" 1) 
COMMENT                               #277=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#5: Product of length 449 (rating: 171)")) 
COMMENT                      #278=(CPCRFolderPar 
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COMMENT                                #279=(CPCRData (CStringList) (CStringList) 
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COMMENT                                      "ctcctgtgggactcgcaaac" 0 171 620 450 0 0) 
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COMMENT                              (CObjectList 
COMMENT                               #280=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 620" 
COMMENT                                     1) 
COMMENT                               #281=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.0 C  TaOpt: 53.7 C  GC: 42.9" 1) 
COMMENT                               #282=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #283=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #284=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #285=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #286=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #287=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #288=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #289=(COlyLinePar 
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COMMENT                                        #290=(COligo "" "CTCCTGTGGGACTCGCAAAC" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CTCCTGTGGGACTCGCAAAC" 1)) 
COMMENT                               #291=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #292=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 54.4 C  GC: 60.0" 1) 
COMMENT                               #293=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -151.3 kcal/mol  dS: -386.6 cal/mol  dG: -34.3 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #294=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 2.9" 1) 
COMMENT                               #295=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 5.0" 1))) 
COMMENT                             "#6: Product of length 450 (rating: 171)")) 
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COMMENT                                     "  Contains region of the molecule from 171 to 644" 
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COMMENT                               #299=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.4 C  TaOpt: 54.0 C  GC: 43.7" 1) 
COMMENT                               #300=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
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COMMENT                                        #302=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #303=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
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COMMENT                               #304=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #305=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #306=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
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COMMENT                                        #308=(COligo "" "AACAGGGAGTTGGCATTGCC" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  AACAGGGAGTTGGCATTGCC" 1)) 
COMMENT                               #309=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #310=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 56.2 C  GC: 55.0" 1) 
COMMENT                               #311=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -159.5 kcal/mol  dS: -407.8 cal/mol  dG: -36.1 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #312=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 4.7" 1) 
COMMENT                               #313=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#7: Product of length 474 (rating: 171)")) 
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COMMENT                                #315=(CPCRData (CStringList) (CStringList) 
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COMMENT                              (CObjectList 
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COMMENT                                     "  Contains region of the molecule from 171 to 645" 
COMMENT                                     1) 
COMMENT                               #317=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.4 C  TaOpt: 54.1 C  GC: 43.8" 1) 
COMMENT                               #318=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
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COMMENT                                        #320=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #321=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #322=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #323=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #324=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #325=(COlyLinePar 
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COMMENT                                        #326=(COligo "" "CAACAGGGAGTTGGCATTGC" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  CAACAGGGAGTTGGCATTGC" 1)) 
COMMENT                               #327=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #328=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 55.3 C  GC: 55.0" 1) 
COMMENT                               #329=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -154.3 kcal/mol  dS: -394.1 cal/mol  dG: -35.0 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #330=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.8" 1) 
COMMENT                               #331=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#8: Product of length 475 (rating: 171)")) 
COMMENT                      #332=(CPCRFolderPar 
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COMMENT                                #333=(CPCRData (CStringList) (CStringList) 
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COMMENT                              (CObjectList 
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COMMENT                                     "  Contains region of the molecule from 171 to 646" 
COMMENT                                     1) 
COMMENT                               #335=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.5 C  TaOpt: 54.1 C  GC: 43.9" 1) 
COMMENT                               #336=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
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COMMENT                                        #338=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #339=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #340=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #341=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #342=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
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COMMENT                                        #344=(COligo "" "GCAACAGGGAGTTGGCATTG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  GCAACAGGGAGTTGGCATTG" 1)) 
COMMENT                               #345=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #346=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 55.3 C  GC: 55.0" 1) 
COMMENT                               #347=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -154.3 kcal/mol  dS: -394.1 cal/mol  dG: -35.0 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #348=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.8" 1) 
COMMENT                               #349=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#9: Product of length 476 (rating: 171)")) 
COMMENT                      #350=(CPCRFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     "  Contains region of the molecule from 171 to 647" 
COMMENT                                     1) 
COMMENT                               #353=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 76.5 C  TaOpt: 54.1 C  GC: 43.8" 1) 
COMMENT                               #354=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
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COMMENT                                        #356=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #357=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #358=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #359=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #360=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #361=(COlyLinePar 
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COMMENT                                        #362=(COligo "" "AGCAACAGGGAGTTGGCATT" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2387" 
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COMMENT                                      "  AGCAACAGGGAGTTGGCATT" 1)) 
COMMENT                               #363=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #364=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 53.6 C  GC: 50.0" 1) 
COMMENT                               #365=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -156.3 kcal/mol  dS: -402.0 cal/mol  dG: -34.7 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #366=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 2.1" 1) 
COMMENT                               #367=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 5.0" 1))) 
COMMENT                             "#10: Product of length 477 (rating: 171)")))) 
COMMENT                    "PCR Analysis")))) 
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FEATURES             Location/Qualifiers
     CDS             4290..5147
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    5321..5909
                     /vntifkey="21"
                     /label=Origin
     misc_feature    3352..3397
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3360..3527)
                     /vntifkey="34"
                     /label=3'\ITR
     intron          1561..1733
                     /vntifkey="15"
                     /label=Intron
     polyA_signal    3067..3318
                     /vntifkey="25"
                     /label=SV40pA
     misc_RNA        2480..3064
                     /vntifkey="53"
                     /label=WPRE\-\mut6
     primer          complement(1657..1678)
                     /vntifkey="27"
                     /label=oEMS5695
     exon            1734..2473
                     /vntifkey="61"
                     /label=emGFP
     primer          complement(1862..1882)
                     /vntifkey="27"
                     /label=oEMS5954
     misc_feature    209..419
                     /vntifkey="21"
                     /label=RR25
     misc_feature    420..777
                     /vntifkey="21"
                     /label=RR18
     misc_feature    778..1006
                     /vntifkey="21"
                     /label=RR7
     promoter        1007..1552
                     /vntifkey="29"
                     /label=RR13
     misc_feature    209..1552
                     /vntifkey="21"
                     /label=Ple389
     primer          complement(652..671)
                     /vntifkey="27"
                     /label=oEMS6325
     primer          complement(598..617)
                     /vntifkey="27"
                     /label=oEMS6326
BASE COUNT     1431 a      1689 c      1695 g      1522 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccag gaggggctga gtaaatctgc gcctgatttt 
      241 catctgccta cactgaatta tgccatccat ttggaggcaa ttttcaagtt aaaatgttgt 
      301 cagaaggctg tcacagtttt tatctttctt ttctgttctt tctcaaccca cccacatttt 
      361 ctagttaggt tggaaagggg agtgaggggg atagagcctg gtttatattc agagatttct 
      421 taacagggcc agaacccagg aagggatatg cttttctcag gaataatgtc aaaattaata 
      481 tagcttccca gtcaatgagg actatataaa ttactatttg caaagcacta agaacagtgt 
      541 ccggcacaga gtcagtgcta tgtaatgtag atgtgagaat gagagagcct gagattttat 
      601 gtttgcgagt cccacaggag gcagggcaat gccaactccc tgttgcttgg aagcaatggg 
      661 caagtctatg accctcctgt tgaggccctg ttacaggtaa ccaagccccc acggaatgga 
      721 gctgaggagc tcctgagaat gggcttgtcc ggttactgtt tggattcaga actgaccgtc 
      781 cacggccacg gtgtcagtcc ctccttgttc tgcgcttgct ttttggggtc tcccagcctc 
      841 tccctagtcc tagagtttct caggcaaatc ccagccacta cccctccatc cgtgtcccct 
      901 gtttccccag tgctgagctg gctgagctgt gagctttcct gctccttgcc tcaggaagcc 
      961 aggggcagcc cagtcacctc tggccagagg cgggccttag gccaagcctg gttccgtacc 
     1021 tgctttctgc cagggaccaa actcccgtta gtcattcctg gtgtcagcca ggcagaggag 
     1081 caggtggggg agagggcgtg ggaggaggga ggtgaggcag ctccctccga gtggaagctc 
     1141 acagtcagct gcagcgccgc ccctgccctg actgcagctg actggcaggg gcacctggag 
     1201 gcagcgggtt cgggcgcagt atgagagggc ttaccctctg cggcagtggg agacaggacc 
     1261 aggcagctcc ccacatcctt ccacatccga gctccagggg tctctggctt gtcctttcac 
     1321 agggcggctg gtgtccctta ggtgggtgct ggtagttcct gggagagttg acgggatgga 
     1381 gcaggccagg ggggctgaga agttggcctg gcgagagaag gtgggtggga ctgggagagc 
     1441 ctgggcaggg aggggccttt ctgggaggga gtgtcatggg gctggccgag atccaggacc 
     1501 caggagactg gccaagctgc cacaggaggc ctgagaggct gagcatgggg ggggcgcgcc 
     1561 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag 
     1621 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta 
     1681 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg 
     1741 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
     1801 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
     1861 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
     1921 ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cgcccgctac cccgaccaca 
     1981 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
     2041 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     2101 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     2161 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga 
     2221 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc 
     2281 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 
     2341 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 
     2401 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 
     2461 agtaagcggc cgcgatcgct caacctctgg attacaaaat ttgtgaaaga ttgactggta 
     2521 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 
     2581 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 
     2641 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 
     2701 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 
     2761 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 
     2821 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 
     2881 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 
     2941 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 
     3001 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 
     3061 ccccgcgatc gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat 
     3121 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 
     3181 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 
     3241 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtagtcgaaa 
     3301 ttcccgataa ggatcttcgt cgacgaattc ccgataagga tcttcctaga gcatggctac 
     3361 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg 
     3421 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 
     3481 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct 
     3541 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 
     3601 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 
     3661 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 
     3901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     4021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 
     4081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 
     4141 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
     4201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
     4261 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
     4321 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
     4381 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
     4441 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
     4501 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 
     4561 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
     4621 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
     4681 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
     4741 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
     4801 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 
     4861 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
     4921 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
     4981 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
     5041 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     5101 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     5161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     5221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     5281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     5341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     5401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     5461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     5521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     5581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     5641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     5701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     5761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     5821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     5881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     5941 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     6001 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 
     6061 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 
     6121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 
     6181 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 
     6241 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 
     6301 acagctatga ccatgattac gccagattta attaagg 
//
