LOCUS       pEMS2382        6005 bp    DNA     circular      5-JUL-2018
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12354486|
COMMENT     VNTDBDATE|-12363420|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2382|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                     "  Contains region of the molecule from 171 to 709" 
COMMENT                                     1) 
COMMENT                               #264=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 83.4 C  TaOpt: 58.9 C  GC: 60.3" 1) 
COMMENT                               #265=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #266=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #267=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #268=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #269=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #270=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #271=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #272=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #273=(COligo "" "GGCCAGTTGTGCTTTCATGG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GGCCAGTTGTGCTTTCATGG" 1)) 
COMMENT                               #274=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #275=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 55.3 C  GC: 55.0" 1) 
COMMENT                               #276=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -154.3 kcal/mol  dS: -394.1 cal/mol  dG: -35.0 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #277=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.8" 1) 
COMMENT                               #278=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#5: Product of length 539 (rating: 171)")) 
COMMENT                      #279=(CPCRFolderPar 
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COMMENT                                #280=(CPCRData (CStringList) (CStringList) 
COMMENT                                      "gccatgctctaggaagatcg" 0 
COMMENT                                      "gggccagttgtgctttcatg" 0 171 710 540 0 0) 
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COMMENT                              (CObjectList 
COMMENT                               #281=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 710" 
COMMENT                                     1) 
COMMENT                               #282=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 83.4 C  TaOpt: 58.9 C  GC: 60.4" 1) 
COMMENT                               #283=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #284=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #285=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #286=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #287=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #288=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #289=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #290=(COlyLinePar 
COMMENT                                     (CLinePar 
COMMENT                                      (CParagraph 0 
COMMENT                                       (17 
COMMENT                                        #291=(COligo "" "GGGCCAGTTGTGCTTTCATG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GGGCCAGTTGTGCTTTCATG" 1)) 
COMMENT                               #292=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #293=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 55.3 C  GC: 55.0" 1) 
COMMENT                               #294=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -154.3 kcal/mol  dS: -394.1 cal/mol  dG: -35.0 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #295=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.8" 1) 
COMMENT                               #296=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 0.0" 1))) 
COMMENT                             "#6: Product of length 540 (rating: 171)")) 
COMMENT                      #297=(CPCRFolderPar 
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COMMENT                                #298=(CPCRData (CStringList) (CStringList) 
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COMMENT                                0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #299=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 763" 
COMMENT                                     1) 
COMMENT                               #300=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 82.7 C  TaOpt: 57.5 C  GC: 58.3" 1) 
COMMENT                               #301=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
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COMMENT                                        #303=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #304=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #305=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #306=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #307=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #308=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #309=(COligo "" "CAGCAAATGTCTTCCACTGA" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CAGCAAATGTCTTCCACTGA" 1)) 
COMMENT                               #310=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #311=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 48.3 C  GC: 45.0" 1) 
COMMENT                               #312=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -140.2 kcal/mol  dS: -362.0 cal/mol  dG: -30.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #313=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.2" 1) 
COMMENT                               #314=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 10.0" 1))) 
COMMENT                             "#7: Product of length 593 (rating: 171)")) 
COMMENT                      #315=(CPCRFolderPar 
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COMMENT                                #316=(CPCRData (CStringList) (CStringList) 
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COMMENT                                      "tcagcaaatgtcttccactg" 0 171 764 594 0 0) 
COMMENT                                0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #317=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 764" 
COMMENT                                     1) 
COMMENT                               #318=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 82.6 C  TaOpt: 57.4 C  GC: 58.2" 1) 
COMMENT                               #319=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #320=(COlyLinePar 
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COMMENT                                        #321=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #322=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #323=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #324=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #325=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #326=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #327=(COligo "" "TCAGCAAATGTCTTCCACTG" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  TCAGCAAATGTCTTCCACTG" 1)) 
COMMENT                               #328=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #329=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 48.3 C  GC: 45.0" 1) 
COMMENT                               #330=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -140.2 kcal/mol  dS: -362.0 cal/mol  dG: -30.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #331=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 3.2" 1) 
COMMENT                               #332=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 10.0" 1))) 
COMMENT                             "#8: Product of length 594 (rating: 171)")) 
COMMENT                      #333=(CPCRFolderPar 
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COMMENT                               (20 
COMMENT                                #334=(CPCRData (CStringList) (CStringList) 
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COMMENT                                      "cctttcagcaaatgtcttcc" 0 171 768 598 0 0) 
COMMENT                                0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #335=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 768" 
COMMENT                                     1) 
COMMENT                               #336=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 82.6 C  TaOpt: 57.6 C  GC: 58.2" 1) 
COMMENT                               #337=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #338=(COlyLinePar 
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COMMENT                                        #339=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #340=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #341=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #342=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #343=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #344=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #345=(COligo "" "CCTTTCAGCAAATGTCTTCC" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CCTTTCAGCAAATGTCTTCC" 1)) 
COMMENT                               #346=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #347=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 49.0 C  GC: 45.0" 1) 
COMMENT                               #348=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -151.3 kcal/mol  dS: -393.5 cal/mol  dG: -32.2 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #349=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 2.6" 1) 
COMMENT                               #350=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 10.0" 1))) 
COMMENT                             "#9: Product of length 598 (rating: 171)")) 
COMMENT                      #351=(CPCRFolderPar 
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COMMENT                                #352=(CPCRData (CStringList) (CStringList) 
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COMMENT                                      "ccggatgtggatttaacctt" 0 171 784 614 0 0) 
COMMENT                                0) 1 2 1 0 182) 
COMMENT                              (CObjectList 
COMMENT                               #353=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Contains region of the molecule from 171 to 784" 
COMMENT                                     1) 
COMMENT                               #354=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Tm: 82.5 C  TaOpt: 58.1 C  GC: 57.8" 1) 
COMMENT                               #355=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Sense Primer: " 1) 
COMMENT                               #356=(COlyLinePar 
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COMMENT                                        #357=(COligo "" "GCCATGCTCTAGGAAGATCG" 
COMMENT                                              "Sense primer for PCR amplification of a region of pEMS2382" 
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COMMENT                                      "  GCCATGCTCTAGGAAGATCG" 1)) 
COMMENT                               #358=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #359=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 51.5 C  GC: 55.0" 1) 
COMMENT                               #360=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -153.6 kcal/mol  dS: -396.9 cal/mol  dG: -33.5 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #361=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Antisense Primer: " 1) 
COMMENT                               #362=(COlyLinePar 
COMMENT                                     (CLinePar 
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COMMENT                                        #363=(COligo "" "CCGGATGTGGATTTAACCTT" 
COMMENT                                              "Antisense primer for PCR amplification of a region of pEMS2382" 
COMMENT                                              0 (CStringList) 0) 1) 1 2 2 0 181) 
COMMENT                                      "  CCGGATGTGGATTTAACCTT" 1)) 
COMMENT                               #364=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Similarity: 100.0%" 1) 
COMMENT                               #365=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  Length: 20  Tm: 50.7 C  GC: 45.0" 1) 
COMMENT                               #366=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "  dH: -159.1 kcal/mol  dS: -413.9 cal/mol  dG: -33.9 kcal/mol" 
COMMENT                                     1) 
COMMENT                               #367=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "Tm Difference: 0.8" 1) 
COMMENT                               #368=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                     "GC Difference: 10.0" 1))) 
COMMENT                             "#10: Product of length 614 (rating: 171)")))) 
COMMENT                    "PCR Analysis")))) 
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COMMENT          #370=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT          #371=(CWidgetStyle "Molecule Label 2" 0 0 1 
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COMMENT                "@L bp" 0) 
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FEATURES             Location/Qualifiers
     CDS             3958..4815
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4989..5577
                     /vntifkey="21"
                     /label=Origin
     misc_feature    3020..3065
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3028..3195)
                     /vntifkey="34"
                     /label=3'\ITR
     intron          1229..1401
                     /vntifkey="15"
                     /label=Intron
     polyA_signal    2735..2986
                     /vntifkey="25"
                     /label=SV40pA
     misc_RNA        2148..2732
                     /vntifkey="53"
                     /label=WPRE\-\mut6
     primer          complement(1325..1346)
                     /vntifkey="27"
                     /label=oEMS5695
     exon            1402..2141
                     /vntifkey="61"
                     /label=emGFP
     primer          complement(1530..1550)
                     /vntifkey="27"
                     /label=oEMS5954
     enhancer        209..505
                     /vntifkey="9"
                     /label=RR5
     enhancer        506..687
                     /vntifkey="9"
                     /label=RR2
     enhancer        688..940
                     /vntifkey="9"
                     /label=E2
     promoter        941..1220
                     /vntifkey="29"
                     /label=P1
     misc_feature    209..1220
                     /vntifkey="21"
                     /label=Ple384
     primer          complement(655..674)
                     /vntifkey="27"
                     /label=oEMS6315
     primer          complement(749..768)
                     /vntifkey="27"
                     /label=oEMS6316
BASE COUNT     1366 a      1645 c      1612 g      1382 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggcctc ctgaggaaaa cctgggctta gtcctccctc 
      241 tggcccagga ggggaccgga ccccacagct ggagggagcc ggcttagctg acagcgagtg 
      301 tattaaaaac aagctttgga gcaaagcgga caagctcagg tgttggtaga gttcatccca 
      361 ggccccaaag taatcacatg gcaaacaagc cctgtctaaa tatcacggcg gctggggcag 
      421 cggcacgcag cggcctggaa atgtcagccg ggggtggggg ctcctccgag ccccgggatt 
      481 agcagaggta cctgaagtga atgcggcctg gtggcctcag agactcgccc tgtgggcgga 
      541 gcagcctcac agccaggtcg aagtcagcac tctgaccctg ccccacgcgg ggagtgggca 
      601 ccagtcccag ggcacagacg tgctgggtga ttaatctggg tgattaagcc tcgggctgag 
      661 aggctgttga gagagaacac gctccatttc catgaaagca caactggccc ggcaggaaac 
      721 cgaattaaaa agcaatattt gtatcagtgg aagacatttg ctgaaaggtt aaatccacat 
      781 ccggcagtgt gggccatgag cctccggcgt ggtgttcatc aggcatgtct ctcctcctgg 
      841 cctgggcacc tgagcactgg ggccgccctg ggcagagctg gggcggggtg ctggggggcc 
      901 tggagctgcc tcaccgaggg atcctcagca gccgaccctg gtctcagccc cccgcagagc 
      961 acgagcccct ggtccccgca agcccgcggg ctgaggatga ttcagacagg gctggggagt 
     1021 gaaggcaatt agattccacg gacgagccct ttctcctgcg cctccctcct tcctcaccca 
     1081 cccccgcctc catcaggcac agcaggcagg ggtgggggat gtaaggaggg gaaggtgggg 
     1141 gacccagagg gggctttgac gtcagctcag cttataagag gctgctgggc cagggctgtg 
     1201 gagacggagc ccggacctcc ggcgcgccga agttggtcgt gaggcactgg gcaggtaagt 
     1261 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga 
     1321 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc 
     1381 tccacaggtg tccactccca ggcggccgcc accatggtga gcaagggcga ggagctgttc 
     1441 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
     1501 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     1561 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg 
     1621 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
     1681 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
     1741 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
     1801 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 
     1861 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc 
     1921 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 
     1981 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 
     2041 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
     2101 atcactctcg gcatggacga gctgtacaag taagcggccg cgatcgctca acctctggat 
     2161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     2221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     2281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     2341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     2401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     2461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     2521 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 
     2581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     2641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     2701 acgagtcgga tctccctttg ggccgcctcc ccgcgatcgc agacatgata agatacattg 
     2761 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 
     2821 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 
     2881 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt 
     2941 aaaacctcta caaatgtggt agtcgaaatt cccgataagg atcttcgtcg acgaattccc 
     3001 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa 
     3061 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 
     3121 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 
     3181 cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga 
     3241 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     3301 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     3361 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     3421 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     3481 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     3541 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     3601 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     3661 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     3721 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     3781 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     3841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     3901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     3961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     4021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     4081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     4141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     4201 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     4261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     4321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     4381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     4441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     4501 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     4561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     4621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     4681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     4741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     4801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     4861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     4921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     4981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     5041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     5101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     5161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     5221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     5281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     5341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     5401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     5461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     5521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     5581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     5641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     5701 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     5761 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
     5821 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
     5881 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
     5941 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc cagatttaat 
     6001 taagg 
//
