LOCUS       Exported                3827 bp ds-DNA     circular SYN 11-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2024 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(49..153)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(487..555)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     627..643
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    671..785
                     /locus_tag="PydfJ115"
                     /label=PydfJ115
     misc_feature    696..715
                     /locus_tag="YdfI binding site (inverted repeat)"
                     /label=YdfI binding site (inverted repeat)
     misc_feature    716..721
                     /locus_tag="-35 region"
                     /label=-35 region
     misc_feature    716..720
                     /locus_tag="-35"
                     /label=-35
     misc_feature    730..735
                     /locus_tag="-35 region(1)"
                     /label=-35 region
                     /label=-35 region(1)
     misc_feature    738..743
                     /locus_tag="-10 region"
                     /label=-10 region
     misc_feature    738..742
                     /locus_tag="-10(1)"
                     /label=-10
                     /label=-10(1)
     misc_feature    750
                     /locus_tag="+1 site"
                     /label=+1 site
     misc_feature    753..758
                     /locus_tag="-10 region(1)"
                     /label=-10 region
                     /label=-10 region(1)
     misc_feature    753..758
                     /locus_tag="-10"
                     /label=-10
     misc_feature    769
                     /locus_tag="+1 site(1)"
                     /label=+1 site
                     /label=+1 site(1)
     misc_feature    786..826
                     /locus_tag="pHP14 mRNA stability hairpin"
                     /label=pHP14 mRNA stability hairpin
     misc_feature    complement(827..832)
                     /locus_tag="BamHI-BglII Scar(4)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(4)
     misc_feature    833..846
                     /locus_tag="RBS-Strong"
                     /label=RBS-Strong
     misc_feature    complement(841..846)
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     CDS             847..1359
                     /codon_start=1
                     /label=nanoLuc
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1372..1401
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    complement(1405..1410)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    1411..1778
                     /locus_tag="TrrnB"
                     /label=TrrnB
                     /note="Terminator"
     misc_feature    complement(1447..1452)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     terminator      1712..1758
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1854..1870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1902..1932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1947..1968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2238..2920)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(join(3015..3827,1..48))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
       61 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
      121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
      181 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
      241 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      301 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      361 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
      421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
      481 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      541 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      601 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtaagatct cggctgaatt
      661 ctaaagatct actgcatatt tgaaaattgc ccaaacgtac atgcccgaat gtacgttttt
      721 ttcatttcat tgtcaactac aatgagaaag aatgtgatca agcaatgtgt tgaaaggaga
      781 ttatcacgtc gactctcgag tgagattgtt gacggtaccg tattttggat ctaggaggaa
      841 ggatctatgg tgtttacgct ggaggatttc gtcggtgact ggcgtcagac agctgggtat
      901 aaccttgacc aggtacttga acaaggcggc gtttccagct tatttcaaaa tctgggggtg
      961 tctgtcacac caattcagcg cattgtcttg tctggggaga atggtcttaa aattgatatt
     1021 catgttatca tcccttacga aggtttgtcc ggtgaccaaa tgggacaaat cgagaaaatt
     1081 ttcaaagtgg tttatccagt ggatgatcat cactttaaag tcattttaca ctatggtacg
     1141 ctggtaatcg acggtgtgac accgaacatg attgattatt tcgggcgtcc gtatgaagga
     1201 atcgccgttt ttgatgggaa gaaaatcacc gtaacaggta ctctgtggaa cggaaacaaa
     1261 atcatcgacg aacgcttgat taaccccgat ggtagtttgt tgttccgtgt tacgattaac
     1321 ggagttacgg gttggcgcct ttgtgagcgt atcttggccg gaagcggaag cgagcagaaa
     1381 ctcatctctg aagaggatct gtaaggatct gaagcttggg cccgaacaaa aactcatctc
     1441 agaagaggat ctgaatagcg ccgtcgacca tcatcatcat catcattgag tttaaacggt
     1501 ctccagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
     1561 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     1621 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     1681 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1741 ctgggccttt cgttttatct gttgtttgtc ggtgaactct gaaaggagga actatatccg
     1801 gatcgagatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
     1861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaa
//
