LOCUS       Exported               10249 bp ds-DNA    circular SYN 13-8ÔÂ-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-PERK(LD)-EGFP-HOTag3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Likun
  TITLE     Direct Submission
  JOURNAL   Exported 2024Äê8ÔÂ13ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2811..5014
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    2821..2843
                     /note="mPERK_F"
     misc_feature    2821..2826
                     /note="KozakSequence"
     misc_feature    2827..4074
                     /note="mPERK_luminalDomain"
     misc_feature    4060..4086
                     /note="mPERKld_EGFP_F/R primer"
     misc_feature    4075..4086
                     /note="GSGSlinker"
     CDS             4087..4797
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     misc_feature    4087..4797
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    4087..4118
                     /note="mPERKld_EGFP_F/R primer"
     misc_feature    4897..4986
                     /note="HO-Tag3"
     misc_feature    4987..4998
                     /note="KDEL"
     promoter        5037..5536
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5557..6156
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6170..6758
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6641..6652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             6965..7598
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7727..7743)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7751..7767
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7775..7805)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7820..7841
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8129..8717)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8888..9748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9749..9853)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    9901..10035
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccgccatgg agcgcgccac ccggcccggg ccgcgcgcgc tgctgctgct tctgttcctg
     2881 ctgctgggct gcgcggcggg gatctcggcg gtcgcgcccg cccgcagttt gcttgctccc
     2941 gcgtcggaga cagtgtttgg cttaggggca gcggccgccc cgacttcggc cgcgcgggtg
     3001 cctgcggtgg caacggccga agtgaccgtg gaggacgccg aggcattgcc ggctgccgct
     3061 ggcgaaccgg agtcacgcgc gacggagccc gatgacgacg tggaactgcg gcctcgcggc
     3121 aggtccttgg taatcatcag cactttagat ggacgaatcg ctgcactgga tgccgagaat
     3181 gatgggaaaa agcagtggga tttggacgtg gggtctggtt ccttggtttc atctagcctc
     3241 agcaagccag aggtgtttgg gaacaagatg atcatcccct ccctggatgg agacctcttc
     3301 cagtgggacc gggaccgaga gagcatggag gccgtcccct tcacggtgga gtccctgctc
     3361 gaatcttcct acaagtttgg agatgatgtt gttctggttg gagggaaatc tctgactaca
     3421 tacggactca gtgcttacag tggaaagctg aggtatatct gttctgcctt gggatgtcgc
     3481 cgatgggata gtgatgaaat ggaagaagag gaagacatct tgcttctgca gcgtacgcag
     3541 aagactgtgc gagctgtcgg gcctcgaagc ggcagtgaga agtggaattt cagtgttggc
     3601 cactttgaac ttcggtatat tccagacatg gaaactagag ccggattcat tgaaagcacc
     3661 tttaaaccgg gtggaaacaa agaagactct aaaattattt cagatgtgga agaacaagaa
     3721 gccaccatgc tggacacagt gataaaagtt tccgttgctg attggaaggt catggcgttt
     3781 agtaggaagg gaggccgcct ggaatgggag taccagtttt gtactcccat cgcgtccgcc
     3841 tggctggtga gggatggcaa ggtcatcccc atcagcctgt ttgatgatac aagttacaca
     3901 gccagcgaag aagccttggg agacgaagaa gacattgtag aggctgctcg gggagccaca
     3961 gagaacagcg tgtacttagg gatgtacaga ggccagctgt acctgcagtc gtccgtcagg
     4021 gtctcagaaa agttccctac aagcccaaag gccttggagt ctgtaaatgg cgaaggtagt
     4081 ggaagcagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4141 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4201 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4261 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4321 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4381 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4441 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4501 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4561 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4621 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4681 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4741 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
     4801 tccggcagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggcagtgct
     4861 ggtggtagtg ctggtggtag tgctggtggc tctagaggag aaatcgcaaa gagtctgaaa
     4921 gaaattgcaa aatccttgaa ggaaatagcg tggtcactga aagaaattgc caagtccctc
     4981 aaagggaaag atgaactgta agggcccggg atcccgcgac tctagataat tctaccgggt
     5041 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     5101 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     5161 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     5221 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     5281 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     5341 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     5401 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     5461 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     5521 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     5581 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5641 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5701 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5761 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5821 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5881 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5941 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6001 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6061 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6121 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct
     6181 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6241 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6301 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6361 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6421 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6481 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6541 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6601 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6661 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6721 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     6781 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     6841 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6901 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     6961 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7021 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7081 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7141 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7201 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7261 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7321 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7381 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7441 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7501 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7561 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7621 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     7681 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7741 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7801 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7861 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7921 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8341 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8401 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8461 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8521 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8581 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8641 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8761 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9001 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9841 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9901 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9961 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10021 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10081 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10141 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10201 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
