LOCUS       Exported               10060 bp ds-DNA    circular SYN 13-8ÔÂ-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-PERK(LD)-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Likun
  TITLE     Direct Submission
  JOURNAL   Exported 2024Äê8ÔÂ13ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2811..4825
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    2821..2843
                     /note="mPERK_F"
     misc_feature    2821..2826
                     /note="KozakSequence"
     misc_feature    2827..4074
                     /note="mPERK_luminalDomain"
     misc_feature    4060..4118
                     /note="mPERKld_EGFP_F/R primer"
     misc_feature    4075..4086
                     /note="GSGSlinker"
     CDS             4087..4797
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     misc_feature    4798..4809
                     /note="KDEL"
     promoter        4848..5347
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5368..5967
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5981..6569
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6452..6463)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             6776..7409
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7538..7554)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7562..7578
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7586..7616)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7631..7652
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7940..8528)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8699..9559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9560..9664)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    9712..9846
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccgccatgg agcgcgccac ccggcccggg ccgcgcgcgc tgctgctgct tctgttcctg
     2881 ctgctgggct gcgcggcggg gatctcggcg gtcgcgcccg cccgcagttt gcttgctccc
     2941 gcgtcggaga cagtgtttgg cttaggggca gcggccgccc cgacttcggc cgcgcgggtg
     3001 cctgcggtgg caacggccga agtgaccgtg gaggacgccg aggcattgcc ggctgccgct
     3061 ggcgaaccgg agtcacgcgc gacggagccc gatgacgacg tggaactgcg gcctcgcggc
     3121 aggtccttgg taatcatcag cactttagat ggacgaatcg ctgcactgga tgccgagaat
     3181 gatgggaaaa agcagtggga tttggacgtg gggtctggtt ccttggtttc atctagcctc
     3241 agcaagccag aggtgtttgg gaacaagatg atcatcccct ccctggatgg agacctcttc
     3301 cagtgggacc gggaccgaga gagcatggag gccgtcccct tcacggtgga gtccctgctc
     3361 gaatcttcct acaagtttgg agatgatgtt gttctggttg gagggaaatc tctgactaca
     3421 tacggactca gtgcttacag tggaaagctg aggtatatct gttctgcctt gggatgtcgc
     3481 cgatgggata gtgatgaaat ggaagaagag gaagacatct tgcttctgca gcgtacgcag
     3541 aagactgtgc gagctgtcgg gcctcgaagc ggcagtgaga agtggaattt cagtgttggc
     3601 cactttgaac ttcggtatat tccagacatg gaaactagag ccggattcat tgaaagcacc
     3661 tttaaaccgg gtggaaacaa agaagactct aaaattattt cagatgtgga agaacaagaa
     3721 gccaccatgc tggacacagt gataaaagtt tccgttgctg attggaaggt catggcgttt
     3781 agtaggaagg gaggccgcct ggaatgggag taccagtttt gtactcccat cgcgtccgcc
     3841 tggctggtga gggatggcaa ggtcatcccc atcagcctgt ttgatgatac aagttacaca
     3901 gccagcgaag aagccttggg agacgaagaa gacattgtag aggctgctcg gggagccaca
     3961 gagaacagcg tgtacttagg gatgtacaga ggccagctgt acctgcagtc gtccgtcagg
     4021 gtctcagaaa agttccctac aagcccaaag gccttggagt ctgtaaatgg cgaaggtagt
     4081 ggaagcagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4141 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4201 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4261 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4321 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4381 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4441 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4501 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4561 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4621 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4681 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4741 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagaaa
     4801 gatgaactgt aagggcccgg gatcccgcga ctctagataa ttctaccggg taggggaggc
     4861 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     4921 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     4981 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5041 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5101 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5161 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5221 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     5281 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5341 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     5401 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5461 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5521 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5581 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5641 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5701 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5761 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5821 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5881 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5941 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
     6001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6361 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6541 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     6601 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     6661 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     6721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     6781 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     6841 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     6901 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     6961 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     7021 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     7081 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     7141 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     7201 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     7261 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7321 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7381 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7441 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     7501 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7561 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7621 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7681 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7741 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9661 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
     9721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9841 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     9901 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     9961 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
    10021 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
