LOCUS       pLenti-MCS-3AID-HA-2A-mTurquoiseBSD 9723 bp    DNA     circular SYN 27-AUG-2024
DEFINITION  pLenti-MCS-3AID-HA-2A-mTurquoiseBSD.
ACCESSION   pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
VERSION     pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(2474..3190)
                     /note="pLannotate"
                     /label="mTurquoise"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(1360..1948)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(2057..2455)
                     /note="pLannotate"
                     /label="BSD"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(7943..8322)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    complement(6643..6876)
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             complement(7545..7725)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(651..831)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(7373..7498)
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(5999..6116)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        complement(4717..5888)
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="99.5"
                     /fragment="False"
                     /other="promoter"
     promoter        8588..8692
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             8693..9553
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(2004..2037)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(838..1338)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="95.8"
                     /match_length="82.7"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(7743..7942)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(7051..7389)
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="22.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3512..3736)
                     /note="pLannotate"
                     /label="IAA17 (fragment)"
                     /database="swissprot"
                     /identity="96.0"
                     /match_length="32.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6166..6266)
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4187..4435)
                     /note="pLannotate"
                     /label="IAA14 (fragment)"
                     /database="swissprot"
                     /identity="62.2"
                     /match_length="36.4"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3989..4168)
                     /note="pLannotate"
                     /label="IAA4 (fragment)"
                     /database="swissprot"
                     /identity="71.0"
                     /match_length="32.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3314..3493)
                     /note="pLannotate"
                     /label="IAA4 (fragment)"
                     /database="swissprot"
                     /identity="71.0"
                     /match_length="32.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    8477..8529
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3719..3982)
                     /note="pLannotate"
                     /label="IAA8 (fragment)"
                     /database="swissprot"
                     /identity="51.1"
                     /match_length="27.4"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1948..1971)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="22.2"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(1340..1359)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.5"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc
     2101 caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat
     2161 cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga
     2221 tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga
     2281 gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt
     2341 cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg
     2401 tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatgctac
     2461 cgctgccgct acccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     2521 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     2581 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     2641 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
     2701 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atgtagttgt
     2761 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     2821 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     2881 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     2941 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg
     3001 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3061 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3121 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3181 tgctcaccat tgggccagga ttctcctcga catctccgca agttaggagg cttccccttc
     3241 cctctccaga tccagcataa tcaggcacat cataagggta tccggcgctg ccagcgtagt
     3301 caggcacatc ataggggtat ttatacattc gcagatcaat tttccgcaag tagggggctc
     3361 cgtccatact tacttttaca aatgctgccg cctcggggcc tccagaactc ttttggcaac
     3421 tgaccattac attcttgcgg tatgatctga cggggggcca accgactact tgagcttttg
     3481 ccgggggttt agctgggtct ttcggacatg cactcttctc tttggaagat ttatacatgc
     3541 ggagatctat tttcctaagg tacggggcgc cgtccatgct aactttaacg aatgctgcag
     3601 cctctggtcc gccactagac ttttgacaag ataccataac attcttgcga taggatctga
     3661 caggcggcca accaactact tgagctttgg cgggtggctt cgcagggtcc ttcgggcagg
     3721 cgctcttttc tttgctgctc ttatacatcc taaggtcgat tttcctcaag tacggcgcac
     3781 catccatgga aacctttaca aacgctgcgg cctctggacc tccgcttgat ttttgacatg
     3841 ataccataac atttttcctg tatgagcgca caggaggcca accgacgacc tgagccttcg
     3901 ccggcggctt tgcagggtct tttggacagg cacttttctc cttactcaat tggccggaaa
     3961 tcgatggtgc ataatcaggc acatcatagg ggtatttata cattcgcaga tcaattttcc
     4021 gcaagtaggg ggctccgtcc atacttactt ttacaaatgc tgccgcctcg gggcctccag
     4081 aactcttttg gcaactgacc attacattct tgcggtatga tctgacgggg ggccaaccga
     4141 ctacttgagc ttttgccggg ggtttagctg ggtctttcgg acatgcactc ttctctttgg
     4201 aagatttata catgcggaga tctattttcc taaggtacgg ggcgccgtcc atgctaactt
     4261 taacgaatgc tgcagcctct ggtccgccac tagacttttg acaagatacc ataacattct
     4321 tgcgatagga tctgacaggc ggccaaccaa ctacttgagc tttggcgggt ggcttcgcag
     4381 ggtccttcgg gcaggcgctc ttttctttgc tgctcttata catcctaagg tcgattttcc
     4441 tcaagtacgg cgcaccatcc atggaaacct ttacaaacgc tgcggcctct ggacctccgc
     4501 ttgatttttg acatgatacc ataacatttt tcctgtatga gcgcacagga ggccaaccga
     4561 cgacctgagc cttcgccggc ggctttgcag ggtcttttgg acaggcactt ttctccttac
     4621 tcaattggcc ggaaatcgat ggtgcataat caggaacatc ataaggatag ccaccgttaa
     4681 cagagacgct tgacccgtct ccatttaaat gctagctcac gacacctgaa atggaagaaa
     4741 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     4801 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     4861 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     4921 aaagcgcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     4981 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     5041 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     5101 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     5161 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     5221 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     5281 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     5341 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     5401 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     5461 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     5521 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     5581 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     5641 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     5701 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     5761 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     5821 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     5881 acgggcacat gcatggcggt aatacggtta tccacgcggc cgcctgagtc aaatgggatc
     5941 tctagagcgg gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa
     6001 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     6061 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
     6121 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
     6181 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     6241 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     6301 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     6361 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     6421 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     6481 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     6541 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     6601 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     6661 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     6721 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     6781 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     6841 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     6901 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     6961 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     7021 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     7081 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     7141 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     7201 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     7261 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     7321 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     7381 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     7441 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     7501 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     7561 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     7621 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     7681 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg
     7741 cgctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     7801 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     7861 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     7921 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     7981 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8041 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8101 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8161 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8221 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     8281 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     8341 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     8401 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     8461 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     8521 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     8581 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8641 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8701 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8761 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8821 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8881 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8941 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9001 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9061 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9121 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9181 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9241 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9721 ttc
//
