LOCUS       pAAV-hSyn-ASAP5fKv-WPRE 6074 bp ds-DNA     circular     20-AUG-2023
DEFINITION  .
ACCESSION   urn.local...14-4c2f3f0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            paav-hsyn-asap3kv-wpre.gb
FEATURES             Location/Qualifiers
     primer_bind     45..62
                     /label="primer_bind"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(67..84)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(346..365)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     511..528
                     /label="primer_bind"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(513..530)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     603..622
                     /label="primer_bind"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          623..642
                     /label="5ITRfwd"
                     /note="sequence: gccttttgctggccttttgc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     source          651..650
                     /label="source"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    651..791
                     /label="misc_feature"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(769..828)
                     /label="5ITRrev"
                     /note="sequence: agggccctctgcagtctagacacacgcgtgcggccgcaggaacccctagtgatggagttg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    806..1290
                     /label="misc_feature"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1280..1333
                     /label=201
                     /note="sequence: gcagtcgagaaggtaccggatccgccaccatggagacgactgtgaggtatgaac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1298..1301
                     /label="infusion"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1309..2586
                     /label="Translation 1309-2586"
                     /translation="METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQDSCSNTIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFFLVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSAGTFKGDNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNTDQMPQMVTLLRVLRIVILIRVIRLASQKKQLEVVT"
     misc_feature    1309..2586
                     /label="ASAP5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1486..1488
                     /label="60R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1498..1500
                     /label="64V"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1507..1509
                     /label="67I"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1516..1518
                     /label="70D"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1519..1521
                     /label="71I"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1528..1530
                     /label="74V"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1531..1533
                     /label="75I"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1600..1602
                     /label="98I"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1675..1677
                     /label="123L"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1687..1689
                     /label="127D"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1696..1698
                     /label="130I"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1708..1710
                     /label="134T"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1717..1719
                     /label="137I"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1729..1731
                     /label="141Y"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1750..2478
                     /label="cpsfGFP3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2482..2484
                     /label="392D"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2513..2547
                     /label="R5-TAIL1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2551..2564
                     /label="R5-TAIL2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2558..2607)
                     /label=207
                     /note="sequence: tgaacctgaagaacccttaagggttaccacttcaagttgtttcttctgtg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2587..2615
                     /label=208
                     /note="sequence: cttaagggttcttcaggttcatctggatc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2587..2820
                     /label="Translation 2587-2820"
                     /translation="LKGSSGSSGSSTRQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRSMSSIDSFISCATDFPEATRF"
     misc_feature    2593..2619
                     /label="GSS*3 linker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2626..2820
                     /label="kv2.1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2803..2848)
                     /label=203
                     /note="sequence: cgataagcttgatatcgaattcttatcaaaagcgggtcgcttccgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2841..3449
                     /label="misc_feature"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2926..2943)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     polyA_signal    3469..3947
                     /label="polyA_signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3484..3501)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3531..3554)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(3854..3871)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     3856..3873
                     /label="primer_bind"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3987..4127
                     /label="misc_feature"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      4219..4525
                     /label="rep_origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     4301..4318
                     /label="primer_bind"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(4345..4362)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4720..4737)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     4793..4815
                     /label="primer_bind"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(4846..4862)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(4913..4930)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5044..5901
                     /label="misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(5051..5068)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     5227..5244
                     /label="primer_bind"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(5230..5247)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     5670..5687
                     /label="primer_bind"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5676..5693)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      6052..645
                     /label="rep_origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      721 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      781 taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc gtatgagtgc
      841 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
      901 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
      961 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     1021 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     1081 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     1141 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     1201 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     1261 tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat ggagacgact
     1321 gtgaggtatg aacaggggtc agagctcact aaaacttcga gctctccaac agcagatgag
     1381 cccacgataa agattgatga tggtcgtgat gagggtaatg aacaagacag ctgttccaat
     1441 accattagga gaaaaatttc cccgtttgtg atgtcatttg gattcagagt atttggagtt
     1501 gtgcttatca ttgtagacAT Catagtggtg attgtggatc tggccatcag tgagaagaaa
     1561 agaggcatta gagagattct tgaaggtgtt TCCctggcta tagcactctt cttccttgtt
     1621 gatgttctca tgagagtgtt tgttgaaggc ttcaagaact atttccggtc caaactgaat
     1681 actttggatg cagtcatagt agtgggcact ctgctaattA ATatgaccta ctccttctct
     1741 GCTggtacat ttAAAggtga taacgtgtat attaccgcgg ataaacagaa aaacggcatt
     1801 aaagcgaact ttaccgtgcg ccataacgtg gaagatggca gcgtgcagct ggcggatcat
     1861 tatcagcaga acaccccgat tggcgatggc ccggtgctgc tgccggataa ccattatctg
     1921 agcacccaga ccgtgctgag caaagatccg aacgaaaaac gcgatcacat ggtgctgctg
     1981 gaatttgtga ccgcagcggg cattacacac ggcatggatg aactgtatgg cggcaccggc
     2041 ggcagcgcga gccagggcga agaactgttt accggcgtgg tgccgattct ggtggaactg
     2101 gatggcgatg tgaacggcca taaatttagc gtgcgcggcg aaggcgaagg cgatgcgacc
     2161 attggcaaac tgaccctgaa atttatttgc accaccggca aactaccggt gccgtggccg
     2221 accctggtga ccaccttaac ctatggcgtg cagtgcttta gccgctatcc ggatcatatg
     2281 aaacgccatg atttttttaa aagcgcgatg ccggaaggct atgtgcagga acgcaccatt
     2341 agctttaaag atgatggcaa atataaaacc cgcgcggtgg tgaaatttga aggcgatacc
     2401 ctggtgaacc gcattgaact gaaaggcacc gattttaaag aagatggcaa cattctgggg
     2461 cataaactgg aatataacac agatcagatg ccgcagatgg ttactcttct tCGAgttctg
     2521 AGAattgtta tcttaataag aGTGATTCGC ctggcttcac agaagaaaca acttgaagtg
     2581 gtaaccctta agggttcttc aggttcatct ggatcttcca cgcgtcagag ccagccgatt
     2641 ctgaacacca aagaaatggc gccgcagagc aaaccgccgg aagaactgga aatgagcagc
     2701 atgccgagcc cggtggcgcc gctgccggcg cgcaccgaag gcgtgattga tatgcgcagc
     2761 atgagcagca ttgatagctt tattagctgc gcgaccgatt ttccggaagc gacccgcttt
     2821 tgataagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     2881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3241 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3361 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3421 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc
     3481 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     3541 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     3601 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     3661 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     3721 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3781 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3841 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3901 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
     3961 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     4021 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     4081 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     4141 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4201 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4261 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4321 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4381 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     4441 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4501 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     4561 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4621 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     4681 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4741 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4801 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4861 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5161 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5221 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5281 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5341 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5401 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5461 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5521 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5581 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5641 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5701 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5761 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5821 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5881 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6061 atcaaaggat cttc
//