LOCUS       Exported                6307 bp ds-DNA     circular SYN 28-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    539..560
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=95.5"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        575..605
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    613..629
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             649..822
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     CDS             complement(1086..1202)
                     /codon_start=1
                     /label=yciV (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=84.6"
                     /note="/match_length=13.3"
                     /note="/other=CDS"
                     /translation="LSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVDKLIPT"
     promoter        1278..1306
                     /label=tac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1314..1330
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             1353..2006
                     /codon_start=1
                     /label=GST26_SCHJA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             2046..3359
                     /codon_start=1
                     /label=Usp27
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=96.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGE
                     KFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRD
                     FFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
                     DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
                     IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAK
                     IKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMA
                     SSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHHTSFIRHHKDQWFKCDDAV
                     ITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY"
     CDS             3419..3458
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=20.8"
                     /note="/other=CDS"
                     /translation="LPRAFR**R*KPL"
     misc_feature    3560..3600
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=misc_feature"
     promoter        3705..3809
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3810..4670
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4841..5429
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    5615..5666
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     promoter        5673..5750
                     /label=lacI promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(5751..6307,1..526)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
       61 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
      121 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
      181 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
      241 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
      301 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
      361 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
      421 aaaaaccacg ctagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      541 atgtaagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      601 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      661 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
      841 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
      901 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg
      961 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
     1021 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
     1081 gatggcgttg gaattagctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag
     1141 ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc
     1201 aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa
     1261 tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
     1321 cggataacaa tttcacacag gaaacagtat tcatgtcccc tatactaggt tattggaaaa
     1381 ttaagggcct tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag
     1441 agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt
     1501 tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg
     1561 ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg
     1621 cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg
     1681 catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc
     1741 tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc
     1801 atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc
     1861 tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg
     1921 ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt
     1981 ttggtggtgg cgaccatcct ccaaaatcgg atctggaagt tctgttccag gggcccctgg
     2041 gatccatgtg taaggactat gtatatgaca aagacattga gcaaattgcc aaagaagagc
     2101 aaggagaagc tttgaaatta caagcctcca cctcaacaga ggtttctcac cagcagtgtt
     2161 cagtgccagg ccttggtgag aaattcccaa cctgggaaac aaccaaacca gaattagaac
     2221 tgctggggca caacccgagg agaagaagaa tcacctccag ctttacgatc ggtttaagag
     2281 gactcatcaa tcttggcaac acgtgcttta tgaactgcat tgtccaggcc ctcacccaca
     2341 cgccgatact gagagatttc tttctctctg acaggcaccg atgtgagatg ccgagtcccg
     2401 agttgtgtct ggtctgtgag atgtcgtcgc tgtttcggga gttgtattct ggaaacccgt
     2461 ctcctcatgt gccctataag ttactgcacc tggtgtggat acatgcccgc catttagcag
     2521 ggtacaggca acaggatgcc cacgagttcc tcattgcagc gttagatgtc ctgcacaggc
     2581 actgcaaagg tgatgatgtc gggaaggcgg ccaacaatcc caaccactgt aactgcatca
     2641 tagaccaaat cttcacaggt ggcctgcagt ctgatgtcac ctgtcaagcc tgccatggcg
     2701 tctccaccac gatagaccca tgctgggaca ttagtttgga cttgcctggc tcttgcacct
     2761 ccttctggcc catgagccca gggagggaga gcagtgtgaa cggggaaagc cacataccag
     2821 gaatcaccac cctcacggac tgcttgcgga ggtttacgag gccagagcac ttaggaagca
     2881 gtgccaaaat caaatgtggt agttgccaaa gctaccagga atctaccaaa cagctcacaa
     2941 tgaataaatt acctgtcgtt gcctgttttc atttcaaacg gtttgaacat tcagcgaaac
     3001 agaggcgcaa gatcactaca tacatttcct ttcctctgga gctggatatg acgccgttta
     3061 tggcctcaag taaagagagc agaatgaatg gacaattgca gctgccaacc aatagtggaa
     3121 acaacgaaaa taagtattcc ttgtttgctg tggttaatca ccaaggaacc ttggagagtg
     3181 gccaccatac cagcttcatc cggcaccaca aggaccagtg gttcaagtgt gatgatgccg
     3241 tcatcactaa ggccagtatt aaggacgtac tggacagtga agggtattta ctgttctatc
     3301 acaaacaggt gctagaacat gagtcagaaa aagtgaaaga aatgaacaca caagcctact
     3361 gaggatcccc ggaattcccg ggtcgactcg agcggccgca tcgtgactga ctgacgatct
     3421 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3541 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     3601 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4021 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     4081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4321 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     4381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5581 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     5641 atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggcgc aaaacctttc
     5701 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     5761 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     5821 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     5881 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     5941 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     6001 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     6061 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     6121 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     6181 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     6241 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     6301 tgttagc
//
