LOCUS       Exported                7864 bp ds-DNA     circular SYN 23-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      784..1372
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1555..1647
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1660..1681
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1696..1726
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1734..1750
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1804..1822
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        1832..2211
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2213..2490
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          2491..3509
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.2"
                     /note="/other=intron"
     CDS             3513..3575
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=76.2"
                     /note="/match_length=51.2"
                     /note="/other=CDS"
                     /translation="LGNVLVIVLSHHFGKEFNSNV"
     CDS             3574..3599
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=32.1"
                     /note="/other=CDS"
                     /translation="YPYDVPDY"
     CDS             3607..4920
                     /codon_start=1
                     /label=USP27X
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGE
                     KFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRD
                     FFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
                     DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
                     IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAK
                     IKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMA
                     SSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAV
                     ITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY"
     CDS             5071..5283
                     /codon_start=1
                     /label=lacY (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.0"
                     /note="/other=CDS"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
                     GIIFAAISLFSLLFQPLFGLLSDKLG"
     polyA_signal    5283..5417
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        5422..5921
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5937..6537
                     /codon_start=1
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=100.3"
                     /note="/other=CDS"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLGDL
                     RAPQPPLLRAARLHRHRRRRGARRTAHLVHDPQARCL"
     polyA_signal    6597..6808
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.2"
                     /note="/other=polyA_signal"
     promoter        6812..6833
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     CDS             6872..7026
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     rep_origin      7057..7485
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        7512..7616
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(7617..7864,1..613)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
       61 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      121 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      181 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      241 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      301 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      361 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      421 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      481 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      541 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      601 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1441 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1501 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1621 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1681 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1741 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttgg ccaagtcggc
     1801 ctctaatacg actcactata gggagctcgt cgacattgat tattgactag ttattaatag
     1861 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1921 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1981 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2041 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     2101 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     2161 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     2221 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     2281 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
     2341 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     2401 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     2461 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
     2521 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     2581 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     2641 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
     2701 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     2761 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     2821 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     2881 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg gggtgagcag ggggtgtggg
     2941 cgcgtcggtc gggctgcaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     3001 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     3061 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     3121 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     3181 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     3241 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     3301 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     3361 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     3421 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     3481 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     3541 ctcatcattt tggcaaagaa ttcaactcga atgtacccat acgatgtgcc agattacgcc
     3601 ggatccatgt gtaaggacta tgtatatgac aaagacattg agcaaattgc caaagaagag
     3661 caaggagaag ctttgaaatt acaagcctcc acctcaacag aggtttctca ccagcagtgt
     3721 tcagtgccag gccttggtga gaaattccca acctgggaaa caaccaaacc agaattagaa
     3781 ctgctggggc acaacccgag gagaagaaga atcacctcca gctttacgat cggtttaaga
     3841 ggactcatca atcttggcaa cacgtgcttt atgaactgca ttgtccaggc cctcacccac
     3901 acgccgatac tgagagattt ctttctctct gacaggcacc gatgtgagat gccgagtccc
     3961 gagttgtgtc tggtctgtga gatgtcgtcg ctgtttcggg agttgtattc tggaaacccg
     4021 tctcctcatg tgccctataa gttactgcac ctggtgtgga tacatgcccg ccatttagca
     4081 gggtacaggc aacaggatgc ccacgagttc ctcattgcag cgttagatgt cctgcacagg
     4141 cactgcaaag gtgatgatgt cgggaaggcg gccaacaatc ccaaccactg taactgcatc
     4201 atagaccaaa tcttcacagg tggcctgcag tctgatgtca cctgtcaagc ctgccatggc
     4261 gtctccacca cgatagaccc atgctgggac attagtttgg acttgcctgg ctcttgcacc
     4321 tccttctggc ccatgagccc agggagggag agcagtgtga acggggaaag ccacatacca
     4381 ggaatcacca ccctcacgga ctgcttgcgg aggtttacga ggccagagca cttaggaagc
     4441 agtgccaaaa tcaaatgtgg tagttgccaa agctaccagg aatctaccaa acagctcaca
     4501 atgaataaat tacctgtcgt tgcctgtttt catttcaaac ggtttgaaca ttcagcgaaa
     4561 cagaggcgca agatcactac atacatttcc tttcctctgg agctggatat gacgccgttt
     4621 atggcctcaa gtaaagagag cagaatgaat ggacaattgc agctgccaac caatagtgga
     4681 aacaacgaaa ataagtattc cttgtttgct gtggttaatc accaaggaac cttggagagt
     4741 ggccactata ccagcttcat ccggcaccac aaggaccagt ggttcaagtg tgatgatgcc
     4801 gtcatcacta aggccagtat taaggacgta ctggacagtg aagggtattt actgttctat
     4861 cacaaacagg tgctagaaca tgagtcagaa aaagtgaaag aaatgaacac acaagcctac
     4921 tgatcgagcc ttaattaagg gctagcaaat gcatgcggcc gccaattcca gctgagcgcc
     4981 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     5041 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg
     5101 atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc
     5161 ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc
     5221 gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc
     5281 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5401 cttatcatgt ctggatctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     5461 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     5521 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     5581 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     5641 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     5701 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     5761 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     5821 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5881 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacaagctag cttaccatga
     5941 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6001 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6061 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6121 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6181 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6241 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6301 cgtggttccc ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6361 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctcggag
     6421 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6481 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6541 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatttgc
     6601 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6661 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6721 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6781 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     6841 acgcggccgg ccgctgacca agtcagcttg gcactggccg tcgttttaca acgtcgtgac
     6901 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6961 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7021 ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7081 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7141 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7201 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7261 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7321 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7381 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7441 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     7501 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7561 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7621 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7681 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7741 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7801 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7861 ttga
//
