LOCUS       Exported                7860 bp ds-DNA     circular SYN 28-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    111..245
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        250..749
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             765..1365
                     /codon_start=1
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=100.2"
                     /note="/other=CDS"
                     /translation="MTEYKPTVRLATRDDVPGAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLGDL
                     RAPQPPLLRAARLHRHRRRRGARRTAHLVHDPQARCL"
     polyA_signal    1425..1636
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.2"
                     /note="/other=polyA_signal"
     promoter        1640..1661
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     CDS             1700..1854
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     rep_origin      1885..2313
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        2340..2444
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2445..3305
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3476..4064
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             4247..4339
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    4352..4373
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4388..4418
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4426..4442
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4496..4514
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        4524..4903
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        4905..5180
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          5181..6197
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             6201..6263
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=76.2"
                     /note="/match_length=51.2"
                     /note="/other=CDS"
                     /translation="LGNVLVIVLSHHFGKEFNSNV"
     CDS             6262..6287
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=32.1"
                     /note="/other=CDS"
                     /translation="YPYDVPDY"
     CDS             6295..7608
                     /codon_start=1
                     /label=Usp27
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=96.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGE
                     KFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRD
                     FFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPCKLLHLVWIHARHLAGYRQQ
                     DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
                     IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAK
                     IKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMA
                     SSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAV
                     ITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY"
     CDS             join(7759..7860,1..111)
                     /codon_start=1
                     /label=lacY (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.0"
                     /note="/other=CDS"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
                     GIIFAAISLFSLLFQPLFGLLSDKLG"
ORIGIN
        1 catgacatca accatatcag caaaagtgat acgggtatta tttttgccgc tatttctctg
       61 ttctcgctat tattccaacc gctgtttggt ctgctttctg acaaactcgg aacttgttta
      121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      241 ggatctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      301 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      361 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      421 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      481 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      541 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      601 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      661 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      721 tctcctcttc ctcatctccg ggcctttcga caagctagct taccatgacc gagtacaagc
      781 ccacggtgcg cctcgccacc cgcgacgacg tccccggggc cgtacgcacc ctcgccgccg
      841 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
      901 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
      961 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     1021 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     1081 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcccgg
     1141 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     1201 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctcggagac ctccgcgccc
     1261 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     1321 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     1381 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatttgcca gccatctgtt
     1441 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1501 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1561 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1621 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac gcggccggcc
     1681 gctgaccaag tcagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1741 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1801 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa
     1861 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     1921 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     1981 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2041 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     2101 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2161 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2221 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2281 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     2341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2461 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2521 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2581 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2641 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2701 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2761 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2821 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2881 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2941 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3001 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3061 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3121 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3181 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3241 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3301 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3361 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3601 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4261 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4321 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4381 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4441 aatttcacac aggaaacagc tatgacatga ttacgaattt ggccaagtcg gcctctaata
     4501 cgactcacta tagggagctc gtcgacattg attattgact agttattaat agtaatcaat
     4561 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4621 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4681 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4741 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4801 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4861 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     4921 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     4981 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg
     5041 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     5101 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     5161 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     5221 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     5281 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     5341 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     5401 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     5461 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     5521 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     5581 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     5641 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     5701 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     5761 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     5821 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     5881 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     5941 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     6001 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     6061 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     6121 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     6181 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     6241 gcaaagaatt caactcgaat gtacccatac gatgtgccag attacgccgg atccatgtgt
     6301 aaggactatg tatatgacaa agacattgag caaattgcca aagaagagca aggagaagct
     6361 ttgaaattac aagcctccac ctcaacagag gtttctcacc agcagtgttc agtgccaggc
     6421 cttggtgaga aattcccaac ctgggaaaca accaaaccag aattagaact gctggggcac
     6481 aacccgagga gaagaagaat cacctccagc tttacgatcg gtttaagagg actcatcaat
     6541 cttggcaaca cgtgctttat gaactgcatt gtccaggccc tcacccacac gccgatactg
     6601 agagatttct ttctctctga caggcaccga tgtgagatgc cgagtcccga gttgtgtctg
     6661 gtctgtgaga tgtcgtcgct gtttcgggag ttgtattctg gaaacccgtc tcctcatgtg
     6721 ccctgtaagt tactgcacct ggtgtggata catgcccgcc atttagcagg gtacaggcaa
     6781 caggatgccc acgagttcct cattgcagcg ttagatgtcc tgcacaggca ctgcaaaggt
     6841 gatgatgtcg ggaaggcggc caacaatccc aaccactgta actgcatcat agaccaaatc
     6901 ttcacaggtg gcctgcagtc tgatgtcacc tgtcaagcct gccatggcgt ctccaccacg
     6961 atagacccat gctgggacat tagtttggac ttgcctggct cttgcacctc cttctggccc
     7021 atgagcccag ggagggagag cagtgtgaac ggggaaagcc acataccagg aatcaccacc
     7081 ctcacggact gcttgcggag gtttacgagg ccagagcact taggaagcag tgccaaaatc
     7141 aaatgtggta gttgccaaag ctaccaggaa tctaccaaac agctcacaat gaataaatta
     7201 cctgtcgttg cctgttttca tttcaaacgg tttgaacatt cagcgaaaca gaggcgcaag
     7261 atcactacat acatttcctt tcctctggag ctggatatga cgccgtttat ggcctcaagt
     7321 aaagagagca gaatgaatgg acaattgcag ctgccaacca atagtggaaa caacgaaaat
     7381 aagtattcct tgtttgctgt ggttaatcac caaggaacct tggagagtgg ccactatacc
     7441 agcttcatcc ggcaccacaa ggaccagtgg ttcaagtgtg atgatgccgt catcactaag
     7501 gccagtatta aggacgtact ggacagtgaa gggtatttac tgttctatca caaacaggtg
     7561 ctagaacatg agtcagaaaa agtgaaagaa atgaacacac aagcctactg atcgagcctt
     7621 aattaagggc tagcaaatgc atgcggccgc caattccagc tgagcgccgg tcgctaccat
     7681 taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct gcccgtattt
     7741 cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat gttcggttta
     7801 ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc gatttggcta
//
