LOCUS       Exported                7862 bp ds-DNA     circular SYN 06-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(677..781)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      808..1236
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1267..1421)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     promoter        complement(1460..1481)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     polyA_signal    complement(1485..1696)
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.2"
                     /note="/other=polyA_signal"
     CDS             complement(1756..2355)
                     /codon_start=1
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=100.2"
                     /note="/other=CDS"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(2371..2871)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.2"
                     /note="/other=promoter"
     polyA_signal    complement(2876..3010)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(3010..3222)
                     /codon_start=1
                     /label=lacY (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.0"
                     /note="/other=CDS"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
                     GIIFAAISLFSLLFQPLFGLLSDKLG"
     CDS             complement(3373..4686)
                     /codon_start=1
                     /label=Usp27
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=96.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGE
                     KFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRD
                     FFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
                     DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
                     IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAK
                     IKCGSCQSYQESTKQLTMNKLPVVACFHFKRVEHSAKQRRKITTYISFPLELDMTPFMA
                     SSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAV
                     ITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY"
     CDS             complement(4694..4719)
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=32.1"
                     /note="/other=CDS"
                     /translation="YPYDVPDY"
     CDS             complement(4718..4780)
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=76.2"
                     /note="/match_length=51.2"
                     /note="/other=CDS"
                     /translation="LGNVLVIVLSHHFGKEFNSNV"
     intron          complement(4784..5800)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        complement(5801..6078)
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        complement(6080..6459)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        complement(6469..6487)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(6541..6557)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(6565..6595)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(6610..6631)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(6644..6736)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(6919..7507)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(7678..7862,1..676))
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
       61 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
      361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      781 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
      841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1261 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1321 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1381 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga cttggtcagc
     1441 ggccggccgc gtcgagcccc agctggttct ttccgcctca gaagccatag agcccaccgc
     1501 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
     1561 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
     1621 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
     1681 aacagatggc tggcaaatcg atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg
     1741 gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     1801 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     1861 gggcgcggag gtctcgagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     1921 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     1981 cgggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     2041 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     2101 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     2161 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     2221 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     2281 ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     2341 cttgtactcg gtcatggtaa gctagcttgt cgaaaggccc ggagatgagg aagaggagaa
     2401 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
     2461 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     2521 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
     2581 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
     2641 catttgtcac gtcctgcacg acgcgagctg cggggcgggg ggggaacttc ctgactaggg
     2701 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
     2761 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
     2821 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagatcc
     2881 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2941 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3001 taaacaagtt ccgagtttgt cagaaagcag accaaacagc ggttggaata atagcgagaa
     3061 cagagaaata gcggcaaaaa taatacccgt atcacttttg ctgatatggt tgatgtcatg
     3121 tagccaaatc gggaaaaacg ggaagtaggc tcccatgata aaaaagtaaa agaaaaagaa
     3181 taaaccgaac atccaaaagt ttgtgttttt taaatagtac ataatggatt tccttacgcg
     3241 aaatacgggc agacatggcc tgcccggtta ttattatttt tgacaccaga ccaactggta
     3301 atggtagcga ccggcgctca gctggaattg gcggccgcat gcatttgcta gcccttaatt
     3361 aaggctcgat cagtaggctt gtgtgttcat ttctttcact ttttctgact catgttctag
     3421 cacctgtttg tgatagaaca gtaaataccc ttcactgtcc agtacgtcct taatactggc
     3481 cttagtgatg acggcatcat cacacttgaa ccactggtcc ttgtggtgcc ggatgaagct
     3541 ggtatagtgg ccactctcca aggttccttg gtgattaacc acagcaaaca aggaatactt
     3601 attttcgttg tttccactat tggttggcag ctgcaattgt ccattcattc tgctctcttt
     3661 acttgaggcc ataaacggcg tcatatccag ctccagagga aaggaaatgt atgtagtgat
     3721 cttgcgcctc tgtttcgctg aatgttcaac ccgtttgaaa tgaaaacagg caacgacagg
     3781 taatttattc attgtgagct gtttggtaga ttcctggtag ctttggcaac taccacattt
     3841 gattttggca ctgcttccta agtgctctgg cctcgtaaac ctccgcaagc agtccgtgag
     3901 ggtggtgatt cctggtatgt ggctttcccc gttcacactg ctctccctcc ctgggctcat
     3961 gggccagaag gaggtgcaag agccaggcaa gtccaaacta atgtcccagc atgggtctat
     4021 cgtggtggag acgccatggc aggcttgaca ggtgacatca gactgcaggc cacctgtgaa
     4081 gatttggtct atgatgcagt tacagtggtt gggattgttg gccgccttcc cgacatcatc
     4141 acctttgcag tgcctgtgca ggacatctaa cgctgcaatg aggaactcgt gggcatcctg
     4201 ttgcctgtac cctgctaaat ggcgggcatg tatccacacc aggtgcagta acttataggg
     4261 cacatgagga gacgggtttc cagaatacaa ctcccgaaac agcgacgaca tctcacagac
     4321 cagacacaac tcgggactcg gcatctcaca tcggtgcctg tcagagagaa agaaatctct
     4381 cagtatcggc gtgtgggtga gggcctggac aatgcagttc ataaagcacg tgttgccaag
     4441 attgatgagt cctcttaaac cgatcgtaaa gctggaggtg attcttcttc tcctcgggtt
     4501 gtgccccagc agttctaatt ctggtttggt tgtttcccag gttgggaatt tctcaccaag
     4561 gcctggcact gaacactgct ggtgagaaac ctctgttgag gtggaggctt gtaatttcaa
     4621 agcttctcct tgctcttctt tggcaatttg ctcaatgtct ttgtcatata catagtcctt
     4681 acacatggat ccggcgtaat ctggcacatc gtatgggtac attcgagttg aattctttgc
     4741 caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg aaaaagaaga
     4801 aggcatgaac atggttagca gaggctctag agccgccggt cacacgccag aagccgaacc
     4861 ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg cggacagccc cgaggctgga
     4921 gagggagaag gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc atttccttcc
     4981 tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg ggtgcggcgg cgcctcccag
     5041 atttcggctc cgcacagatt tgggacaaag gaagtccctg cgccctctcg cacgattacc
     5101 ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc gccggcgctc cgggggccgc
     5161 cgcgcccctc ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca
     5221 cctgccgcca ccccccgccc ggcacggcga gccccgcgcc acgccccgta cggagccccg
     5281 cacccgaagc cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg ggttgcagcc
     5341 cgaccgacgc gcccacaccc cctgctcacc cccccacgca cacaccccgc acgcagcctt
     5401 tgttcccctc gcagcccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc
     5461 gcacactgcg gagcgcacaa agccccgcgc cgcgcccgca gcgctcacag ccgccgggca
     5521 gcgcggagcc gcacgcggcg ctccccacgc acacacacac gcacgcaccc cccgagccgc
     5581 tccccccgca caaagggccc tcccggagcc cctcaaggct ttcacgcagc cacagaaaag
     5641 aaacaagccg tcattaaacc aagcgctaat tacagcccgg aggagaaggg ccgtcccgcc
     5701 cgctcacctg tgggagtaac gcggtcagtc agagccgggg cgggcggcgc gaggcggcgg
     5761 cggagcgggg cacggggcga aggcagcgcg cagcgactcc cgcccgccgc gcgcttcgct
     5821 ttttataggg ccgccgccgc cgccgcctcg ccataaaagg aaactttcgg agcgcgccgc
     5881 tctgattggc tgccgccgca cctctccgcc tcgccccgcc ccgcccctcg ccccgccccg
     5941 ccccgcctgg cgcgcgcccc cccccccccc ccgcccccat cgctgcacaa aataattaaa
     6001 aaataaataa atacaaaatt gggggtgggg agggggggga gatggggaga gtgaagcaga
     6061 acgtggggct cacctcgacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     6121 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     6181 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     6241 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     6301 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     6361 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     6421 taattgatta ctattaataa ctagtcaata atcaatgtcg acgagctccc tatagtgagt
     6481 cgtattagag gccgacttgg ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     6541 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6601 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6721 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6781 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6901 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7321 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7681 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7741 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7801 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7861 gc
//
