LOCUS       HA-USP27X_Y381H         7863 bp    DNA     circular SYN 02-AUG-2024
DEFINITION  HA-USP27X_Y381H.
ACCESSION   HA-USP27X_Y381H
VERSION     HA-USP27X_Y381H
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        4639..5138
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(6273..6701)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        1049..1428
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    4500..4634
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6728..6832
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1710..2726
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             6833..7693
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2824..4137
                     /note="pLannotate"
                     /label="Usp27"
                     /database="swissprot"
                     /identity="96.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             5154..5753
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1430..1709
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.7"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     polyA_signal    5813..6024
                     /note="pLannotate"
                     /label="bGH poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.2"
                     /fragment="True"
                     /other="polyA_signal"
     promoter        1021..1039
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6088..6242
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     CDS             4288..4500
                     /note="pLannotate"
                     /label="lacY (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="17.0"
                     /fragment="True"
                     /other="CDS"
     CDS             2791..2816
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="32.1"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             2724..2792
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="82.6"
                     /match_length="15.6"
                     /fragment="True"
                     /other="CDS"
     promoter        6028..6049
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aatttggcca agtcggcctc
     1021 taatacgact cactataggg agctcgtcga cattgattat tgactagtta ttaatagtaa
     1081 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1141 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1201 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1261 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1321 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1381 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     1441 cccacgttct gcttcactct ccccatctcc ccccccctcc ccacccccaa ttttgtattt
     1501 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg gggcgcgcgc
     1561 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     1621 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     1681 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     1741 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     1801 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     1861 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     1921 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     1981 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     2041 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     2101 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     2161 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2221 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2281 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2341 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     2401 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     2461 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     2521 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     2581 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     2641 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     2701 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     2761 atcattttgg caaagaattc aactcgaatg tacccatacg atgtgccaga ttacgccgga
     2821 tccatgtgta aggactatgt atatgacaaa gacattgagc aaattgccaa agaagagcaa
     2881 ggagaagctt tgaaattaca agcctccacc tcaacagagg tttctcacca gcagtgttca
     2941 gtgccaggcc ttggtgagaa attcccaacc tgggaaacaa ccaaaccaga attagaactg
     3001 ctggggcaca acccgaggag aagaagaatc acctccagct ttacgatcgg tttaagagga
     3061 ctcatcaatc ttggcaacac gtgctttatg aactgcattg tccaggccct cacccacacg
     3121 ccgatactga gagatttctt tctctctgac aggcaccgat gtgagatgcc gagtcccgag
     3181 ttgtgtctgg tctgtgagat gtcgtcgctg tttcgggagt tgtattctgg aaacccgtct
     3241 cctcatgtgc cctataagtt actgcacctg gtgtggatac atgcccgcca tttagcaggg
     3301 tacaggcaac aggatgccca cgagttcctc attgcagcgt tagatgtcct gcacaggcac
     3361 tgcaaaggtg atgatgtcgg gaaggcggcc aacaatccca accactgtaa ctgcatcata
     3421 gaccaaatct tcacaggtgg cctgcagtct gatgtcacct gtcaagcctg ccatggcgtc
     3481 tccaccacga tagacccatg ctgggacatt agtttggact tgcctggctc ttgcacctcc
     3541 ttctggccca tgagcccagg gagggagagc agtgtgaacg gggaaagcca cataccagga
     3601 atcaccaccc tcacggactg cttgcggagg tttacgaggc cagagcactt aggaagcagt
     3661 gccaaaatca aatgtggtag ttgccaaagc taccaggaat ctaccaaaca gctcacaatg
     3721 aataaattac ctgtcgttgc ctgttttcat ttcaaacggt ttgaacattc agcgaaacag
     3781 aggcgcaaga tcactacata catttccttt cctctggagc tggatatgac gccgtttatg
     3841 gcctcaagta aagagagcag aatgaatgga caattgcagc tgccaaccaa tagtggaaac
     3901 aacgaaaata agtattcctt gtttgctgtg gttaatcacc aaggaacctt ggagagtggc
     3961 caccatacca gcttcatccg gcaccacaag gaccagtggt tcaagtgtga tgatgccgtc
     4021 atcactaagg ccagtattaa ggacgtactg gacagtgaag ggtatttact gttctatcac
     4081 aaacaggtgc tagaacatga gtcagaaaaa gtgaaagaaa tgaacacaca agcctactga
     4141 tcgagcctta attaagggct agcaaatgca tgcggccgcc aattccagct gagcgccggt
     4201 cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag gccatgtctg
     4261 cccgtatttc gcgtaaggaa atccattatg tactatttaa aaaacacaaa cttttggatg
     4321 ttcggtttat tctttttctt ttactttttt atcatgggag cctacttccc gtttttcccg
     4381 atttggctac atgacatcaa ccatatcagc aaaagtgata cgggtattat ttttgccgct
     4441 atttctctgt tctcgctatt attccaaccg ctgtttggtc tgctttctga caaactcgga
     4501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4621 atcatgtctg gatctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4681 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4741 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4801 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4861 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4921 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4981 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5041 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5101 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac aagctagctt accatgaccg
     5161 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5221 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5281 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5341 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5401 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5461 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5521 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5581 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5641 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5701 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     5761 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atttgccagc
     5821 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5881 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5941 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6001 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacgc
     6061 ggccggccgc tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     6121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6241 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6301 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6361 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6421 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6481 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6541 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6601 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6661 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     6721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7681 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7741 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7861 ttc
//
