LOCUS       Exported                7859 bp ds-DNA     circular SYN 11-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      775..1363
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1546..1638
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1651..1672
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1687..1717
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1725..1741
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1795..1813
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        1823..2202
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2204..2479
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          2480..3496
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             3500..3562
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=76.2"
                     /note="/match_length=51.2"
                     /note="/other=CDS"
                     /translation="LGNVLVIVLSHHFGKEFNSNV"
     CDS             3561..3586
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=32.1"
                     /note="/other=CDS"
                     /translation="YPYDVPDY"
     CDS             3594..4907
                     /codon_start=1
                     /label=Usp27
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=96.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGE
                     KFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRD
                     FFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
                     DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
                     IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAK
                     IKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMA
                     SSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAV
                     ITKANIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY"
     CDS             5058..5270
                     /codon_start=1
                     /label=lacY (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.0"
                     /note="/other=CDS"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
                     GIIFAAISLFSLLFQPLFGLLSDKLG"
     polyA_signal    5270..5404
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        5409..5908
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5924..6523
                     /codon_start=1
                     /label=PuroR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MTEYKPTVRLATRDDVPGAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    6583..6794
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.2"
                     /note="/other=polyA_signal"
     promoter        6798..6819
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     CDS             6858..7012
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     rep_origin      7043..7471
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        7498..7602
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(7603..7859,1..604)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
       61 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1441 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1501 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1561 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1621 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1681 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1741 atttcacaca ggaaacagct atgacatgat tacgaatttg gccaagtcgg cctctaatac
     1801 gactcactat agggagctcg tcgacattga ttattgacta gttattaata gtaatcaatt
     1861 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1921 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1981 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2041 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2101 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2161 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     2221 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     2281 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcgc caggcggggc
     2341 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     2401 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     2461 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     2521 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     2581 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     2641 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     2701 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     2761 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     2821 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     2881 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     2941 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     3001 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     3061 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     3121 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3181 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3241 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3301 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3361 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     3421 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     3481 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     3541 caaagaattc aactcgaatg tacccatacg atgtgccaga ttacgccgga tccatgtgta
     3601 aggactatgt atatgacaaa gacattgagc aaattgccaa agaagagcaa ggagaagctt
     3661 tgaaattaca agcctccacc tcaacagagg tttctcacca gcagtgttca gtgccaggcc
     3721 ttggtgagaa attcccaacc tgggaaacaa ccaaaccaga attagaactg ctggggcaca
     3781 acccgaggag aagaagaatc acctccagct ttacgatcgg tttaagagga ctcatcaatc
     3841 ttggcaacac gtgctttatg aactgcattg tccaggccct cacccacacg ccgatactga
     3901 gagatttctt tctctctgac aggcaccgat gtgagatgcc gagtcccgag ttgtgtctgg
     3961 tctgtgagat gtcgtcgctg tttcgggagt tgtattctgg aaacccgtct cctcatgtgc
     4021 cctataagtt actgcacctg gtgtggatac atgcccgcca tttagcaggg tacaggcaac
     4081 aggatgccca cgagttcctc attgcagcgt tagatgtcct gcacaggcac tgcaaaggtg
     4141 atgatgtcgg gaaggcggcc aacaatccca accactgtaa ctgcatcata gaccaaatct
     4201 tcacaggtgg cctgcagtct gatgtcacct gtcaagcctg ccatggcgtc tccaccacga
     4261 tagacccatg ctgggacatt agtttggact tgcctggctc ttgcacctcc ttctggccca
     4321 tgagcccagg gagggagagc agtgtgaacg gggaaagcca cataccagga atcaccaccc
     4381 tcacggactg cttgcggagg tttacgaggc cagagcactt aggaagcagt gccaaaatca
     4441 aatgtggtag ttgccaaagc taccaggaat ctaccaaaca gctcacaatg aataaattac
     4501 ctgtcgttgc ctgttttcat ttcaaacggt ttgaacattc agcgaaacag aggcgcaaga
     4561 tcactacata catttccttt cctctggagc tggatatgac gccgtttatg gcctcaagta
     4621 aagagagcag aatgaatgga caattgcagc tgccaaccaa tagtggaaac aacgaaaata
     4681 agtattcctt gtttgctgtg gttaatcacc aaggaacctt ggagagtggc cactatacca
     4741 gcttcatccg gcaccacaag gaccagtggt tcaagtgtga tgatgccgtc atcactaagg
     4801 ccaatattaa ggacgtactg gacagtgaag ggtatttact gttctatcac aaacaggtgc
     4861 tagaacatga gtcagaaaaa gtgaaagaaa tgaacacaca agcctactga tcgagcctta
     4921 attaagggct agcaaatgca tgcggccgcc aattccagct gagcgccggt cgctaccatt
     4981 accagttggt ctggtgtcaa aaataataat aaccgggcag gccatgtctg cccgtatttc
     5041 gcgtaaggaa atccattatg tactatttaa aaaacacaaa cttttggatg ttcggtttat
     5101 tctttttctt ttactttttt atcatgggag cctacttccc gtttttcccg atttggctac
     5161 atgacatcaa ccatatcagc aaaagtgata cgggtattat ttttgccgct atttctctgt
     5221 tctcgctatt attccaaccg ctgtttggtc tgctttctga caaactcgga acttgtttat
     5281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5401 gatctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     5461 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     5521 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     5581 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     5641 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     5701 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     5761 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     5821 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     5881 ctcctcttcc tcatctccgg gcctttcgac aagctagctt accatgaccg agtacaagcc
     5941 cacggtgcgc ctcgccaccc gcgacgacgt ccccggggcc gtacgcaccc tcgccgccgc
     6001 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6061 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6121 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6181 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6241 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcccggc
     6301 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6361 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6421 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6481 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     6541 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atttgccagc catctgttgt
     6601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6721 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6781 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacgc ggccggccgc
     6841 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     7021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7141 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7201 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7261 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7321 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7381 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7441 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     7501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggca
//
