LOCUS       pGLU_63                 2332 bp ds-DNA     circular     08-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label="upstream region"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter ID ... 20..39
                     /label=">skpp-1908-F_sk20mer-9990"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(40..69)
                     /label="R primer binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     prokaryote p... 70..253
                     /label="PtetL"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    254..319
                     /label="downstream region"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(376..392)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     ORI             complement(564..1152)
                     /label="ColE1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      1285..1379
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(1400..2059)
                     /label="cat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(2060..2162)
                     /label="Pcat"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2264..2280
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 GTGACTCAGG TCTCACGTAA GTATCTGCTA CGGCTAGCCG TTCCGCAGCC ACACGCCGTG
       61 TGAAGCTGGG GATTTTATGA CCGATGATGA AGAAAAGAAT CTGAAACTTA GTTTATATGT
      121 GGTAAAATGT TTTAATCAAG TTTAGGAGGA ATTAATTATG AAGTGTAATT AATGTAACAG
      181 GGTTCAATTA AAAGAGGGAA GCGTATCATT AACCCTATAA ACTACGTCTG CCCTCATTAT
      241 TGGAGGGTGA AATAATGTGA GACCGGGATT CACATTGGAT ACGAGATCCG TAGATTGATA
      301 AGAGGACACG GAATATCCCA GGCTAGGTGG AGGCTCAGTG ATGATAAGTC TGCGATGGTG
      361 GATGCATGTG TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCAGAGGGCA
      421 CAATCCTATT CCGCGCTATC CGACAATCTC CAAGACATTA GGTGGAGTTC AGTTCGGCGT
      481 ATGGCATATG TCGCTGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
      541 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
      601 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
      661 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
      721 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
      781 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
      841 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
      901 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
      961 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
     1021 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     1081 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     1141 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCT ATTCAACAAA
     1201 GCCGCCGTCC CGTCAAGTCA GCGTAAATGG GTAGGGGGCT TCAAATCGTC CCCCCATACG
     1261 ATATAAGTTG TTACTAGTGC TTGGATTCTC ACCAATAAAA AACGCCCGGC GGCAACCGAG
     1321 CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT GGATCTATCA ACAGGAGTCC
     1381 AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT
     1441 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA
     1501 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA
     1561 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG
     1621 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT
     1681 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC
     1741 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC
     1801 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG AAATTCCGGA TGAGCATTCA
     1861 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG
     1921 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG
     1981 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC
     2041 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA
     2101 ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT
     2161 CAACGTCTCA TTGATACCTG AAACAAAACC CATCGTACGG CCAAGGAAGT CTCCAATAAC
     2221 TGTGATCCAC CACAAGCGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT
     2281 CATGCATAAT CCGCACGCAT CTGGAATAAG GAAGTGCCAT TCCGCCTGAC CT
//