LOCUS       pGLU_9                  5548 bp ds-DNA     circular     19-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..17
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     prokaryote t... 70..99
                     /label="L2U5H11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     miscellaneous   100..139
                     /label="UNS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     miscellaneous   complement(159..404)
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     prokaryote t... complement(405..585)
                     /label="TAmarelleT9"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(590..1636)
                     /label="Himar_Tnp"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     prokaryote p... 1649..1778
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     RBS             1779..1814
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1815..2513
                     /label="mScarlet-I"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 2514..2603
                     /label="ECK120029600"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2617..3351
                     /label="ermC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="codon-optimized to allow Twist synthesis"
     prokaryote t... 3356..3441
                     /label="TermC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     miscellaneous   3442..3471
                     /label="Himar_RE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   complement(3442..3471)
                     /label="Himar_LE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   3491..3530
                     /label="UNSX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     prokaryote p... 3531..3562
                     /label="L2U8H11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(3619..3635)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     ORI             3825..4213
                     /label="R6K ORI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(4470..5330)
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(5331..5435)
                     /label="Pamp"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 GTAAAACGAC GGCCAGTCAT GCATAATCCG CACGCATCTG GAATAAGGAA GTGCCATTCC
       61 GCCTGACCTT AGCGTGCGAA CAGCACGCTA TTGTTGTATC ATTACTCGCA TCCATTCTCA
      121 GGCTGTCTCG TCTCGTCTCC GAGCTCTTCA ATGAGGAGTA CGGCCAGCCT CGCAGAGCAG
      181 GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG
      241 CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT
      301 CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA
      361 AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGTCGTTG GGATTGTCCT
      421 GACCGAAGCG GGACAGGTCC ACGAGCAGGC AATGCTTGTT GGGCATGGTG AGGTGGATGT
      481 CTTTCACTTC CGGCACGCAG CCGAGGACTT TTTGCGCCAT ATCGTAAAGT GTGTGCTGAA
      541 CGGATTCGCT TTCGTGTTCA GCGAAAGAGG CGAGAAGGAG ATTCCAAGCT TATTCAACAT
      601 AGTTCCCTTC AAGAGCGATA CAACGATTAT AACGACCTTC CAATTTTTTG ATACCATTTT
      661 GGTAGTACTC CTTCGGTTTT GCCTCAAAAT AGGCCTCAGT TTCGGCGATC ACCTCTTCAT
      721 TGCAGCCAAA TTTTTTCCCT GCGAGCATCC TTTTGAGGTC TGAGAACAAG AAAAAGTCGC
      781 TGGGGGCCAG ATCTGGAGAA TACGGCGGGT GGGGAAGCAA TTCGAAGCCC AATTCATGAA
      841 TTTTTGCCAT CGTTCTCAAT GACTTGTGGC ACGGTGCGTT GTCTTGGTGG AACAACACTT
      901 TTTTCTTCTT CATGTGGGGC CGTTTTGCCG CGATTTCGAC CTTCAAACGC TCCAATAACG
      961 CCATATAATA GTCACTGTTG ATGGTTTTTC CCTTCTCAAG ATAATCGATA AAAATTATTC
     1021 CATGCGCATC CCAAAAAACA GAGGCCATTA CTTTGCCAGC GGACTTTTGA GTCTTTCCAC
     1081 GCTTCGGAGA CGGTTCACCG GTCGCTGTCC ACTCAGCCGA CTGTCGATTG GACTCAGGAG
     1141 TGTAGTGATG GAGCCATGTT TCATCCATTG TCACATATCG ACGGAAAAAC TCGGGTGTAT
     1201 TACGAGTTAA CAGCTGCAAA CACCGCTTAG AATCATCAAC ACGTCGTTGT TTTTGGTCAA
     1261 ATGTGAGCTC GCGCGGCACC CATTTCGCAC AGAGCTTCCG CATATCCAAA TATTGATGAA
     1321 TGATATGACC AACACGTTCC TTTGATATCT TTAAGGCCTC TGCTATCTCG ATCAACTTCA
     1381 TTTTACGGTC ATTCAAAATC ATTTTGTGGA TTTTTTTGAT GTTTTCGTCG GTAACCACCT
     1441 CTTTCGGGCG TCCACTGCGT TCACCGTCCT CCGTGCTCAT TTCACCACGC TTGAATTTTG
     1501 CATACCAATC AATTATTGTT GATTTCCCTG GGGCAGAGTC CGGAAACTCA TTATCAAGCC
     1561 AAGTTTTTGC TTCCACTGTA TTTTTTCCCT TCAGAAAACA GTATTTTATC AAAACACGAA
     1621 ATTCCTTTTT TTCCATTGAG ACCCAGTCGA AAGTGAAACG TGATTTCATG CGTCATTTTG
     1681 AACATTTTGT AAATCTTATT TAATAATGTG TGCGGCAATT CACATTTAAT TTATGAATGT
     1741 TTTCTTAACA TCGCGGCAAC TCAAGAAACG GCAGGTTCGG ATCTTAGCTA CTAGAGAAAG
     1801 AGGAGAAATA CTAGATGGTG AGTAAGGGTG AAGCCGTGAT TAAAGAGTTC ATGCGCTTCA
     1861 AAGTGCATAT GGAGGGCTCA ATGAACGGCC ATGAATTTGA AATTGAAGGT GAAGGAGAAG
     1921 GCCGTCCGTA TGAGGGCACC CAGACCGCCA AACTGAAAGT CACAAAGGGT GGCCCGCTGC
     1981 CGTTTTCGTG GGATATTTTA TCCCCTCAAT TCATGTACGG CAGCCGGGCG TTTATTAAAC
     2041 ACCCGGCGGA TATCCCGGAT TACTATAAGC AATCTTTTCC AGAAGGCTTT AAATGGGAGC
     2101 GCGTGATGAA TTTTGAAGAC GGAGGTGCTG TTACCGTGAC CCAGGACACG AGCTTAGAAG
     2161 ACGGTACCTT GATCTATAAG GTAAAGCTGC GTGGTACCAA TTTCCCGCCG GATGGGCCAG
     2221 TTATGCAGAA AAAGACTATG GGCTGGGAAG CATCGACGGA GCGTCTTTAC CCCGAAGACG
     2281 GCGTCCTGAA AGGCGATATT AAAATGGCCC TGCGACTGAA AGACGGCGGT CGCTACCTTG
     2341 CAGATTTCAA AACAACGTAT AAAGCGAAAA AACCTGTGCA GATGCCCGGG GCGTACAACG
     2401 TTGATCGCAA ACTAGATATC ACTTCACACA ACGAAGATTA CACGGTAGTC GAACAGTATG
     2461 AACGTAGCGA GGGGCGCCAT AGCACCGGAG GCATGGATGA ACTCTATAAA TAATTCAGCC
     2521 AAAAAACTTA AGACCGCCGG TCTTGTCCAC TACCTTGCAG TAATGCGGTG GACAGGATCG
     2581 GCGGTTTTCT TTTCTCTTCT CAAGACTGGG TCTCAAATGA ACGAGAAAAA TATAAAACAC
     2641 AGTCAAAACT TTATTACTTC TAAGCATAAT ATCGACAAGA TTATGACAAA CATAAGATTA
     2701 AATGAACATG ATAATATCTT TGAAATCGGC TCAGGAAAAG GCCATTTTAC CCTTGAATTA
     2761 GTAAAGAGGT GTAATTTCGT AACTGCCATT GAAATAGACC ATAAATTATG CAAAACTACA
     2821 GAAAATAAAC TTGTTGATCA CGATAATTTC CAAGTTTTAA ACAAGGATAT ATTGCAGTTT
     2881 AAATTTCCTA AAAACCAATC CTATAAAATA TATGGTAATA TACCTTATAA CATAAGTACG
     2941 GATATAATAC GCAAAATTGT TTTTGATAGT ATAGCTAATG AGATTTATTT AATCGTGGAA
     3001 TACGGGTTTG CTAAAAGATT ATTAAATACA AAACGCTCAT TGGCATTACT TTTAATGGCA
     3061 GAAGTTGATA TTTCTATATT AAGTATGGTT CCAAGAGAAT ATTTTCATCC TAAACCTAAA
     3121 GTGAATAGCT CACTTATCAG ATTAAGTAGA AAAAAATCAA GAATATCACA CAAAGATAAA
     3181 CAAAAGTATA ATTATTTCGT TATGAAATGG GTTAACAAAG AATACAAGAA AATATTTACA
     3241 AAAAATCAAT TTAACAATTC CTTAAAACAT GCAGGAATTG ACGATTTAAA CAATATTAGC
     3301 TTTGAACAAT TCTTATCTCT TTTCAATAGC TATAAATTAT TTAATAAGTA AGCTTGTTAA
     3361 GGGATGCATA AACTGCATCC CTTAACTTGT TTTTCGTGTG CCTATTTTTT GTGAATCGAT
     3421 TATGTCTTTT GCGCAGTCGG CTAGACCGGG GACTTATCAT CCAACCTGTT ACGCTAGCAT
     3481 GAAGAGCACC CCAGGATACA TAGATTACCA CAACTCCGAG CCCTTCCACC TAGCGTGCTA
     3541 ACCACGCACG CTATTGTTGT ATAGGCTAGG TGGAGGCTCA GTGATGATAA GTCTGCGATG
     3601 GTGGATGCAT GTGTCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCAGAGG
     3661 GCACAATCCT ATTCCGCGCT ATCCGACAAT CTCCAAGACA TTAGGTGGAG TTCAGTTCGG
     3721 CGTATGGCAT ATGTCGCTGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     3781 CGTAAAAAGG CCGCGTTGCT GGCGTTACAG CTGGGCGCGC CCCATGTCAG CCGTTAAGTG
     3841 TTCCTGTGTC ACTCAAAATT GCTTTGAGAG GCTCTAAGGG CTTCTCAGTG CGTTACATCC
     3901 CTGGCTTGTT GTCCACAACC GTTAAACCTT AAAGGCTTTA AAAGCCTTAT ATATTCTTTT
     3961 TTTTCTTATA AAACTTAAAA CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA
     4021 TTGTTCAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA
     4081 GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG TACGTTAAAC
     4141 ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA CTATCAACAG GTTGAACTGC
     4201 TGATCTTCAG ATCGGCGCGC CGGCCGGCCG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
     4261 ACGCTCTATT CAACAAAGCC GCCGTCCCGT CAAGTCAGCG TAAATGGGTA GGGGGCTTCA
     4321 AATCGTCCTC GTGATACCAA TTCGGAGCCT GCTTTTTTGT ACAAACTTGT TGATAATGGC
     4381 AATTCAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     4441 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     4501 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     4561 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAGCCACGC
     4621 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     4681 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     4741 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     4801 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     4861 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     4921 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     4981 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     5041 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     5101 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     5161 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     5221 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     5281 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     5341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     5401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCAGAT
     5461 ACCTGAAACA AAACCCATCG TACGGCCAAG GAAGTCTCCA ATAACTGTGA TCCACCACAA
     5521 GCGCCAGGGT TTTCCCAGTC ACGACGTT
//