LOCUS       pGLU_12                 5590 bp ds-DNA     circular     18-MAR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..17
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     prokaryote t... 70..99
                     /label="L2U5H11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     miscellaneous   100..139
                     /label="UNS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     miscellaneous   complement(159..404)
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     prokaryote t... complement(405..585)
                     /label="TAmarelleT9"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(590..1636)
                     /label="Himar_Tnp"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     prokaryote p... 1649..1778
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     RBS             1779..1814
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1815..2513
                     /label="mScarlet-I"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     prokaryote t... 2514..2603
                     /label="ECK120029600"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2617..3372
                     /label="aad(9)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="codons changed to allow synthesis"
     prokaryote t... 3377..3483
                     /label="Taad(9)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     miscellaneous   3484..3513
                     /label="Himar_RE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   complement(3484..3513)
                     /label="Himar_LE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     miscellaneous   3533..3572
                     /label="UNSX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     prokaryote p... 3573..3604
                     /label="L2U8H11"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(3661..3677)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     ORI             3867..4255
                     /label="R6K ORI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(4512..5372)
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(5373..5477)
                     /label="Pamp"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 GTAAAACGAC GGCCAGTCAT GCATAATCCG CACGCATCTG GAATAAGGAA GTGCCATTCC
       61 GCCTGACCTT AGCGTGCGAA CAGCACGCTA TTGTTGTATC ATTACTCGCA TCCATTCTCA
      121 GGCTGTCTCG TCTCGTCTCC GAGCTCTTCA ATGAGGAGTA CGGCCAGCCT CGCAGAGCAG
      181 GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG
      241 CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT
      301 CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA
      361 AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGTCGTTG GGATTGTCCT
      421 GACCGAAGCG GGACAGGTCC ACGAGCAGGC AATGCTTGTT GGGCATGGTG AGGTGGATGT
      481 CTTTCACTTC CGGCACGCAG CCGAGGACTT TTTGCGCCAT ATCGTAAAGT GTGTGCTGAA
      541 CGGATTCGCT TTCGTGTTCA GCGAAAGAGG CGAGAAGGAG ATTCCAAGCT TATTCAACAT
      601 AGTTCCCTTC AAGAGCGATA CAACGATTAT AACGACCTTC CAATTTTTTG ATACCATTTT
      661 GGTAGTACTC CTTCGGTTTT GCCTCAAAAT AGGCCTCAGT TTCGGCGATC ACCTCTTCAT
      721 TGCAGCCAAA TTTTTTCCCT GCGAGCATCC TTTTGAGGTC TGAGAACAAG AAAAAGTCGC
      781 TGGGGGCCAG ATCTGGAGAA TACGGCGGGT GGGGAAGCAA TTCGAAGCCC AATTCATGAA
      841 TTTTTGCCAT CGTTCTCAAT GACTTGTGGC ACGGTGCGTT GTCTTGGTGG AACAACACTT
      901 TTTTCTTCTT CATGTGGGGC CGTTTTGCCG CGATTTCGAC CTTCAAACGC TCCAATAACG
      961 CCATATAATA GTCACTGTTG ATGGTTTTTC CCTTCTCAAG ATAATCGATA AAAATTATTC
     1021 CATGCGCATC CCAAAAAACA GAGGCCATTA CTTTGCCAGC GGACTTTTGA GTCTTTCCAC
     1081 GCTTCGGAGA CGGTTCACCG GTCGCTGTCC ACTCAGCCGA CTGTCGATTG GACTCAGGAG
     1141 TGTAGTGATG GAGCCATGTT TCATCCATTG TCACATATCG ACGGAAAAAC TCGGGTGTAT
     1201 TACGAGTTAA CAGCTGCAAA CACCGCTTAG AATCATCAAC ACGTCGTTGT TTTTGGTCAA
     1261 ATGTGAGCTC GCGCGGCACC CATTTCGCAC AGAGCTTCCG CATATCCAAA TATTGATGAA
     1321 TGATATGACC AACACGTTCC TTTGATATCT TTAAGGCCTC TGCTATCTCG ATCAACTTCA
     1381 TTTTACGGTC ATTCAAAATC ATTTTGTGGA TTTTTTTGAT GTTTTCGTCG GTAACCACCT
     1441 CTTTCGGGCG TCCACTGCGT TCACCGTCCT CCGTGCTCAT TTCACCACGC TTGAATTTTG
     1501 CATACCAATC AATTATTGTT GATTTCCCTG GGGCAGAGTC CGGAAACTCA TTATCAAGCC
     1561 AAGTTTTTGC TTCCACTGTA TTTTTTCCCT TCAGAAAACA GTATTTTATC AAAACACGAA
     1621 ATTCCTTTTT TTCCATTGAG ACCCAGTCGA AAGTGAAACG TGATTTCATG CGTCATTTTG
     1681 AACATTTTGT AAATCTTATT TAATAATGTG TGCGGCAATT CACATTTAAT TTATGAATGT
     1741 TTTCTTAACA TCGCGGCAAC TCAAGAAACG GCAGGTTCGG ATCTTAGCTA CTAGAGAAAG
     1801 AGGAGAAATA CTAGATGGTG AGTAAGGGTG AAGCCGTGAT TAAAGAGTTC ATGCGCTTCA
     1861 AAGTGCATAT GGAGGGCTCA ATGAACGGCC ATGAATTTGA AATTGAAGGT GAAGGAGAAG
     1921 GCCGTCCGTA TGAGGGCACC CAGACCGCCA AACTGAAAGT CACAAAGGGT GGCCCGCTGC
     1981 CGTTTTCGTG GGATATTTTA TCCCCTCAAT TCATGTACGG CAGCCGGGCG TTTATTAAAC
     2041 ACCCGGCGGA TATCCCGGAT TACTATAAGC AATCTTTTCC AGAAGGCTTT AAATGGGAGC
     2101 GCGTGATGAA TTTTGAAGAC GGAGGTGCTG TTACCGTGAC CCAGGACACG AGCTTAGAAG
     2161 ACGGTACCTT GATCTATAAG GTAAAGCTGC GTGGTACCAA TTTCCCGCCG GATGGGCCAG
     2221 TTATGCAGAA AAAGACTATG GGCTGGGAAG CATCGACGGA GCGTCTTTAC CCCGAAGACG
     2281 GCGTCCTGAA AGGCGATATT AAAATGGCCC TGCGACTGAA AGACGGCGGT CGCTACCTTG
     2341 CAGATTTCAA AACAACGTAT AAAGCGAAAA AACCTGTGCA GATGCCCGGG GCGTACAACG
     2401 TTGATCGCAA ACTAGATATC ACTTCACACA ACGAAGATTA CACGGTAGTC GAACAGTATG
     2461 AACGTAGCGA GGGGCGCCAT AGCACCGGAG GCATGGATGA ACTCTATAAA TAATTCAGCC
     2521 AAAAAACTTA AGACCGCCGG TCTTGTCCAC TACCTTGCAG TAATGCGGTG GACAGGATCG
     2581 GCGGTTTTCT TTTCTCTTCT CAAGACTGGG TCTCAAATGT TGAATACATA CGAACAAATT
     2641 AATAAAGTGA AAAAAATACT TCGGAAACAT TTAAAAAATA ACCTTATTGG TACTTACATG
     2701 TTTGGATCAG GAGTTGAGAG TGGACTAAAA CCAAATAGTG ATCTTGACTT TTTAGTCGTC
     2761 GTATCTGAAC CATTGACAGA TCAAAGTAAA GAAATACTTA TACAAAAAAT TAGACCTATT
     2821 TCAAAGAAAA TAGGAGATAA AAGCAACTTA CGATATATTG AATTAACAAT TATTATTCAG
     2881 CAAGAAATGG TACCGTGGAA TCATCCTCCC AAACAAGAAT TTATTTATGG AGAATGGTTA
     2941 CAAGAGCTTT ATGAACAAGG ATACATTCCT CAGAAGGAAT TAAATTCAGA TTTAACCATA
     3001 ATGCTTTACC AAGCAAAACG AAAAAATAAA AGAATATACG GAAATTATGA CTTAGAGGAA
     3061 TTACTACCTG ATATTCCATT TTCTGATGTG AGACGCGCCA TTATGGATTC GTCAGAGGAA
     3121 TTAATAGATA ATTATCAGGA TGATGAAACC AACTCTATAT TAACTTTATG CCGTATGATT
     3181 TTAACTATGG ACACGGGTAA AATCATACCA AAAGATATTG CGGGAAATGC AGTGGCTGAA
     3241 TCTTCTCCAT TAGAACATAG GGAGAGAATT TTGTTAGCAG TTCGTAGTTA TCTTGGAGAG
     3301 AATATTGAAT GGACAAACGA GAACGTCAAC CTTACTATAA ATTACTTAAA TAACAGATTA
     3361 AAAAAATTAT AAGCTTAAAA ATTGAAAAAA TGGTGGAAAC ACTTTTTTCA ATTTTTTTGT
     3421 TTTATTATTT AATATTTGGG AAATATTCAT TCTAATTGGT AATCAGATTT TAGAAGTTTA
     3481 AACTAGACCG GGGACTTATC ATCCAACCTG TTACGCTAGC ATGAAGAGCA CCCCAGGATA
     3541 CATAGATTAC CACAACTCCG AGCCCTTCCA CCTAGCGTGC TAACCACGCA CGCTATTGTT
     3601 GTATAGGCTA GGTGGAGGCT CAGTGATGAT AAGTCTGCGA TGGTGGATGC ATGTGTCATG
     3661 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAGA GGGCACAATC CTATTCCGCG
     3721 CTATCCGACA ATCTCCAAGA CATTAGGTGG AGTTCAGTTC GGCGTATGGC ATATGTCGCT
     3781 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     3841 CTGGCGTTAC AGCTGGGCGC GCCCCATGTC AGCCGTTAAG TGTTCCTGTG TCACTCAAAA
     3901 TTGCTTTGAG AGGCTCTAAG GGCTTCTCAG TGCGTTACAT CCCTGGCTTG TTGTCCACAA
     3961 CCGTTAAACC TTAAAGGCTT TAAAAGCCTT ATATATTCTT TTTTTTCTTA TAAAACTTAA
     4021 AACCTTAGAG GCTATTTAAG TTGCTGATTT ATATTAATTT TATTGTTCAA ACATGAGAGC
     4081 TTAGTACGTG AAACATGAGA GCTTAGTACG TTAGCCATGA GAGCTTAGTA CGTTAGCCAT
     4141 GAGGGTTTAG TTCGTTAAAC ATGAGAGCTT AGTACGTTAA ACATGAGAGC TTAGTACGTG
     4201 AAACATGAGA GCTTAGTACG TACTATCAAC AGGTTGAACT GCTGATCTTC AGATCGGCGC
     4261 GCCGGCCGGC CGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCTA TTCAACAAAG
     4321 CCGCCGTCCC GTCAAGTCAG CGTAAATGGG TAGGGGGCTT CAAATCGTCC TCGTGATACC
     4381 AATTCGGAGC CTGCTTTTTT GTACAAACTT GTTGATAATG GCAATTCAAG GATCTTCACC
     4441 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     4501 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     4561 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     4621 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAGCCAC GCTCACCGGC TCCAGATTTA
     4681 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     4741 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     4801 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     4861 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     4921 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     4981 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     5041 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     5101 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     5161 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     5221 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     5281 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     5341 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     5401 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     5461 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAG ATACCTGAAA CAAAACCCAT
     5521 CGTACGGCCA AGGAAGTCTC CAATAACTGT GATCCACCAC AAGCGCCAGG GTTTTCCCAG
     5581 TCACGACGTT
//