LOCUS       159_pAAV-CaMKII(        6723 bp DNA     circular SYN 15-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Lab of Structural Physiology
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    156..442
                     /label=CaMKII(0.3)
     CDS             468..1184
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1197..1517
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     gene            1587..2819
                     /gene="Kalirin"
                     /label=Kalirin-7 C region
     misc_feature    2840..3448
                     /label=WPRE
     misc_feature    2850..3438
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    3469..3946
                     /label=hGHpolyA
     polyA_signal    3470..3946
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3986..4126
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        4938..5042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5043..5903
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6074..6662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttct gggcccacac agtcctgcag
      181 tattgtgtat ataaggccag ggcaaagagg agcaggtttt aaagtgaaag gcaggcaggt
      241 gttggggagg cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg
      301 gacctggatg ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag
      361 ccccaagctc gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt
      421 ggcccctagt tctgggggca gcggggctag cgctaccggt cgccaccatg gtgagcaagg
      481 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      541 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      601 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      661 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      721 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      781 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      841 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      901 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      961 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     1021 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     1081 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1141 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagagtgct ggtggtggag
     1201 tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct
     1261 gcgtggtgca ctacaccggg atgcttgaag atggaaagaa atttgattcc tcccgggaca
     1321 gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag
     1381 gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct
     1441 atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg
     1501 agcttctaaa actggaaagt gctggcggta gtgctggtgg tagtgctggc ggaagtgcag
     1561 gtggcagtgc tggaggtggg atcctaatcc cagcaagcct ttcagaccgg gaggtcaagc
     1621 tccgggatgc taaccacgag gtcaatgaag agaaacggaa gtcagctcga aagaaagaat
     1681 tcattatggc tgaactgctc cagaccgaga aggcttatgt aagagaccta cacgagtgct
     1741 tggagaccta cctgtgggaa atgaccagtg gtgtggaaga gataccgcct gggatcctca
     1801 ataaggagca catcatcttc ggcaacatcc aggagatcta tgacttccac aacaacatct
     1861 tcctcaaaga gctggagaag tacgagcagc tgcctgagga tgtgggacac tgctttgtta
     1921 cctgggcaga caaatttcag atgtatgtca cctactgtaa aaacaagcct gattccaacc
     1981 agctgatcct ggagcacgca ggcaccttct ttgatgagat ccagcagcgg cacgggctgg
     2041 ccaactccat ctcttcctac ttaattaagc ctgtccagag agttacgaag taccagctgc
     2101 tcctgaagga acttttaact tgctgtgaag aagggaaagg ggagctcaag gatggcctgg
     2161 aggtgatgct cagtgtcccc aagaaagcca atgatgccat gcatgtcagc atgcttgaag
     2221 ggttcgatga gaacttggat gtacaggggg agttgattct ccaggatgcc tttcaagtgt
     2281 gggacccaaa gtcgctgatc cggaaggggc gggaacggca tctgttcctc tttgagatct
     2341 ccttggtgtt tagcaaagag atcaaagact cttcaggaca cacgaaatat gtttacaaga
     2401 acaagctact gacctcagag ctgggtgtga ccgagcacgt agaaggtgac ccctgcaaat
     2461 tcgccttgtg gtctgggcgc accccatcct cagacaataa aacagtgctc aaagcttcca
     2521 acatcgaaac caagcaggaa tggatcaaga acattcggga agtgattcag gaaaggatca
     2581 ttcatcttaa aggagcttta aaggagccaa tccagctccc caaaacacca gccaaactga
     2641 ggaacaatag taagagggat ggggtagagg atggtgacag ccagggggac gggagcagcc
     2701 agcccgatac catatccatt gcctccagga cctctcagaa cacagtggag agtgacaagg
     2761 atggcaacct tgttcctcgg tggcacctgg gacctggaga tcctttctcc acttacgttt
     2821 agtaagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     2881 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2941 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3001 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3061 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3121 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3181 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3241 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga
     3301 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3361 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3421 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc
     3481 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     3541 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     3601 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     3661 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     3721 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     3781 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     3841 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     3901 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt
     3961 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     4021 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4081 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     4141 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4201 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4261 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4321 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4381 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4441 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4501 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     4561 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4621 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     4681 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4741 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4801 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6181 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6721 tgt
//
