LOCUS       pTipi2.1                3840 bp    DNA     circular UNA 23-OCT-2024
DEFINITION  .
ACCESSION   urn.local...f-i2b0ih7
VERSION     urn.local...f-i2b0ih7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Regulatory      149..528
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV enhancer (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV enhancer (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_enhancer (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="CMV enhancer"
     promoter        150..726
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:379/6;
                     CMV_promoter (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="CMV_immearly_promoter"
     promoter        696..815
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /label="CMV2_promoter"
     intron          828..960
                     /note="/vntifkey=15"
                     /note="Derived using Geneious Prime 2021.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     beta globin/IgG chimaeric intron (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     beta globin/IgG chimaeric intron (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:yw-g6w6vhi"">MCS_design_w_intron_w_AZU1
                     _SP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:xk-g6vvcqy"">PDL_fragement_with_Intron_
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:x3-g6vtng2"">MCS_design_w_intron</a>)"
                     /label="beta globin/IgG chimaeric intron"
     regulatory      1031..1619
                     /created_by="Haisun"
                     /note="Derived using Geneious Prime 2021.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     WPRE (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     WPRE (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; WPRE (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a href=""urn:local:.:y-g61avr6"">3_pTipi3-PDL1 -
                     WPRE</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="WPRE"
     polyA_signal    1685..1733
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation
                     signal"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; HSV TK
                     poly(A) signal polyA signal (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; HSV TK
                     poly(A) signal polyA signal (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; HSV
                     TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV_TK_polyA (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="HSV TK poly(A) signal polyA signal"
     rep_origin      complement(1962..2635)
                     /created_by="User"
                     /X-Transferred_From="pUC57"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pUC origin (<a href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /label="pUC origin"
     CDS             complement(2780..3640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="2_pTipi1_1_AMP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/3;
                     AmpR (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/3;
                     AmpR (<a href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/3; AmpR
                     (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/3; AmpR
                     (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="AmpR"
     Promoter        complement(3641..3745)
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pUC57"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="AmpR promoter"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactaga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      781 gacctccata gaagacaccg ggaccgatcc agcctccgga cctgcaggta agtatcaagg
      841 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      901 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      961 gtcgactagt gaattctcta gaggtaccct cgagaagctt gctagcggcc gcgagctctg
     1021 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1081 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1141 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     1201 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     1261 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     1321 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     1381 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     1441 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     1501 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     1561 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     1621 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     1681 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcattgcgt
     1741 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2461 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3781 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
