LOCUS       Exported                9473 bp ds-DNA     circular SYN 22-JUL-2024
DEFINITION  hR2-20.
ACCESSION   hR2-20
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..826
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    complement(828..962)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(982..1117)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(1141..1163)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             1170..1209
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1258..1288
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1296..1312
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(1697..1752)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1824..1895)
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     CDS             complement(2313..3813)
                     /label=SEAP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
     misc_feature    complement(3826..4288)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4399..4417
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.4"
                     /note="/other=promoter"
     CDS             complement(4429..5148)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(5156..5182)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=34.6"
                     /note="/other=misc_feature"
     promoter        complement(5189..5385)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    5402..8304
                     /label=hRegion2
     promoter        8338..8442
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             8443..9303
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagattc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     1861 accaccttct gataggcagc ctgcacctga ggagtgcggc cggccgcgat atcctcgacc
     1921 ccactgggct gtaggtggtg gctggatggc ctgagccccc tcctttcctg gggtgcaggc
     1981 agccacaggg cacaacctgc agtccctggg gttggggtag ggagtccgag agcagccaag
     2041 gggcaggttt ggtgtcatgg gctcagtccc cagagcgcac ggggagggga aggttccatg
     2101 gcaggacctc tgtataggga ctccggggat gacgactcgg gctggaggcc aggcaggacg
     2161 acaggaggtg gggagcagga gaactccggg atggggaagc aggagcccca gggacgggac
     2221 actcagggag cagtggccgt ctccagcagc agcagggtcc cggccagcag aggaagcaac
     2281 gcggggacca cggaccgccc cgggtgcgcg gcgtcggtgg tgccggcggg gggcgccagg
     2341 tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc tatgaaggtc
     2401 tgctcctgca cgccgtgaac caggtgcgcc tgcgggccgc gcgcgaacac cgccacgtcc
     2461 tcgcctgcgt gggtctcttc gtccaggggc actgctgact gctgccgata ctcggggctc
     2521 ccgctctcgc tctcggtaac atccggccgg gcgccgtcct tgagcacata gcctggaccg
     2581 tttccgtata ggaggaccgt gtaggccttc ctgtcccggg ccttgccagg ggccagcccg
     2641 aagatggagc tccctcgcag ggggtagcct ccgaaggaga agacgtggga gtggtcggca
     2701 gtgacgaggc tcagcgtgtc ctcctcgctg gtgagctggc ccgccctctc aatggcgtcg
     2761 tcgaacatga tcgtctcagt cagtgcccgg taagccctgc tttcatgatg accatggtcg
     2821 atgcgaccac cctccacgaa gaggaagaag ccgcgggggt tcctgctcag caggcgcagg
     2881 gcagcctctg tcatctccat cagggagggg tccagtgtgg agtctcggtg gatctcgtat
     2941 ttcatgtctc caggctcaaa gagacccatg agatgggtca cagacgggtc cagggaagcc
     3001 tgcatgagct cagtgcggtt ccacacatac cgggcaccct ggcgcttcgc cagccattcc
     3061 tgcaccagat tcttcccgtc cagcctggtc ccaccttggc tgtagtcatc tgggtactca
     3121 gggtctgggg ttcccatgcg aaacatgtac tttcggcctc cacctaggat cacgtcaatg
     3181 tccatgttgg agatgagctg cgtagcgatg tcctggcacc cctcctggcg ggccgaggca
     3241 ggcacgtcgg cgtccgagta ccagttgcgg ttcaccgtgt gggcgtaggt gccggctggc
     3301 gaggcgtgct gcactcgtgt ggtggttacc actcccactg acttccctgc tttcttggcc
     3361 cgattcatca cggagatgac ctcgttgccg cgtgtcgtgt tgcactggtt aaagcgggcg
     3421 gctgcactca agccaatggt ctggaagttg cccttgaccc cgcacaggta ggccgtggct
     3481 gtggctccac tgtctggcac atgtttgtct acattgtatg tcttggacag agccacatat
     3541 gggaagcggt ccatggccag gggtatctca ggccccagtt tgtccttctt ctgccctttt
     3601 aggatcctgg cagctgtcac cgtagacacc cccatcccat cgcccaggaa gatgatgagg
     3661 ttcttggcgg ctgtctgtgc aggctgcagc ttcttggcgg cacccagggc ctcggctgcc
     3721 tcgcggttcc agaagtccgg gttctcctcc tcaactggga tgatgcccag ggagagctgt
     3781 agcctcaggc ccagcagcag cagcagcagc agcctcgacc ccatattatc atcgtgtttt
     3841 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     3901 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     3961 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     4021 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     4081 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     4141 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     4201 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     4261 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     4321 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     4381 ggggggggga gggagagggg gggggggggg ggggcggaat tggccgcttt acttgtacag
     4441 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     4501 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     4561 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     4621 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     4681 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     4741 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     4801 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     4861 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     4921 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     4981 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     5041 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     5101 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg
     5161 tggatcccgg gcccgcggta ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     5221 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     5281 aaattagtca gcgatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     5341 gcggagttag gggcgggact atggttgctg actaattgag atgcagatcg cagatctcga
     5401 gatttaattc tcacaaaatc ctatggagta ggtactatta ttatatacac ccattgtata
     5461 gatgaggaaa ctgaggcaca gagaaattag taacatgccc cacccaggtc atacagcaat
     5521 aagaggtgga cctgggatgc atacgcaggc agtctgactc caaactgctc ctcttaacca
     5581 ctttgttctg tctccatcta tccaccttta cctaaaattt tgtccttctc tatattctcc
     5641 acagcaatga cttgccttat ttttaaaggc ataatcacag tgtgtttggg gagcaaagaa
     5701 aggagcgcat aacctacact agggcaatca cagaggcttt ttggaggtgg tgtctttatt
     5761 tgttcaatga tgtggtagag caaccactgt gtgcccaaca ctggtgatac agacatgaag
     5821 aagccatgga gctcacaaac gggggcctga acaccccatc ggcagtgctc agtgcactga
     5881 tggagaaaac acagggcatc gtagaaacac tggggacacc ccaaagccca ggagatcagg
     5941 gaagacttcc tggaggagag gcactagtcc ccttcactag atctagtcat taacctgagg
     6001 ttcagatggc ccagaggtcc cactgggcac actcaccctt ctttgtctca gccagtccat
     6061 cctccgtgga ccgtctggac caaccaagct tgagaccctc tctgaccacc tgtcctagac
     6121 aggtagaccc cctcagggga agtctgtgtt ccctactgct atgtgttctt agaggcgggg
     6181 cctgagcagg aacgaaactc ctgaacccca gatcctatgc ctagcagggt ctggtgtaag
     6241 tcattggctg ccttgggaaa gcccagtggt ttgattcaag gatgactaga atttagagca
     6301 aaaagggacc ccagaagcca cccaaacctg ttttgaagga agccacagga gatgcccacg
     6361 ccccacagct cagagatgct gcaaattcca gcctcccagt gccctatctt ctctctgggg
     6421 tggtctagcc ccacctggca ctcccccagt ctttgtcccc gtgtctgcct tgcctgatgg
     6481 tggccagggg gcctttccgg aataacgatg gtattggctc agattaaggg caactagctg
     6541 gctcttggct tttcattttt atataacaac taatctcctt ctaatcagcc attattaaat
     6601 tatattccat ttttaattca ttcacagatc accgttggga cagctggatg gataattgga
     6661 gagagataat ttatgtaaat gaggagcagc agaaggtggg ggagagggag gcccagggcg
     6721 ccaaacggag ccccctctgt gtccaggctg gggagggggc agtggcctgg ctcctcctgg
     6781 aaacccgcgc ctggggatgc tgggaggatt gggggctgcc atgggacctc agggctcctg
     6841 accggtggtt cctggtgtgg cccctcagca tcaccttgaa cacttttggc aaatgggaag
     6901 ctcactactt tgggggcgga gtggctctca gacatcagct ccccctttta gaagcagatc
     6961 ctgagaggca gaatgcccca gaaaattctg gagtcagagc tgtttttgat tccaactcca
     7021 ccactgtgtg ccgtttccta accttagttc ccacgtctgc aaaacgcagg taatgacagt
     7081 atctacctca tagagtgctg tgaagcttaa gtgcctggca cctaaggaag agaccaagac
     7141 aagtacatgg tggctcttat cacccttata ggacagtgtg tatctttcag aatcttcctc
     7201 tctaacatct gccttctggc catggagctt ccttctggta acacacaaaa gaaatccact
     7261 tcctttgcat atctgaagac agctctgtac cactgataat aacactccgg aggcttgtgt
     7321 ctcattgtgt cctccgtgtc tcatagtgtc tcattgtatc ctcggaacga ctatagaaat
     7381 tgggggcgat tgcgtccatt ttacagatga atatactgtg gacccaagct ctctcctgcc
     7441 aattccttca acaaaaacga taacaagccc cagaagcctg cttagaaagg aacacgaggc
     7501 tcagaaaacc tggatcccag ccggaggcta ttttgggaag gtgccttggg gactggcagg
     7561 cagggtcagg gatggcaggg aaggtgggtg agagaggttg gacctccacc cttcttggct
     7621 gtccctttgg ggccccaaga atcaggcacg atgggcattc atgggcacag gtggtctgac
     7681 agagcttgag gggcaaggtc tgcctgtgcc tggggctggc atgggctggc agggaggccc
     7741 ttggaccatt cccagaggga gacaggggat taaggggggt tagggaggtg gtcccagagg
     7801 gtccatgcag agtaatggga ttagctcttg ggcagtgagt gaaagttatt atcgtgtggg
     7861 gcctctggcc tcccacccaa ggctgcagct gtcacccatc ctgggaggca ccaacagccc
     7921 cctccacccc caccttgggg caggctggca cctcttctcc ccacaggctt ttctgctgtg
     7981 gtcatctgcc taggggctgg ccgcctagct ttagcctcct gtcggtcctt ggcagactgg
     8041 gccacccaca cagtatgctc tagacttgct ctgtgcctcg gtttcccctt gaacagaaag
     8101 atacaagaag gtgcttgaga tcctctgagg catgggggtt cggagccagg ccccctgggt
     8161 ttccggctct gccacgcatt cgctgtgtga ccctaggtac atctgtcagc ctctctgtgt
     8221 cctaattggt aaaatggggg cagtagcaga gcagcgttag ggttttcata gcggagggct
     8281 gggtagggag catagggtcg gcctgtcgac ccaggtggca cttttcgggg aaatgtgcgc
     8341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8401 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8461 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8521 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8581 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8641 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8701 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8761 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8821 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8881 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8941 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9001 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9061 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9121 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9181 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9241 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9301 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9421 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
